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Measurements of the duodenum and ileum; villus height (a), crypt depth (b), total mucosa (a+b), villus width (c). Crossman's triple stain, bar = 100 µm.

Measurements of the duodenum and ileum; villus height (a), crypt depth (b), total mucosa (a+b), villus width (c). Crossman's triple stain, bar = 100 µm.

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Different challenges are being applied in the poultry industry in order to protect animal health and to increase immunity and production. The supplementation of microbial phytase is essential in terms of both reducing the inorganic phytase rate and contributing to the absorption of other minerals. In this study, a newly isolated microbial phytase w...

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... mucosa thickness was measured from the top of the villus to the lower limit of the crypt. Villus width was measured at the midsection of the villi in the longitudinal section ( Figure 1). Villus absorption area (2 π [average villus width/2] x villus height) was calculated as described previously [32]. ...

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... The highest cost in the poultry industry was related to the nutrition sector. Therefore, using feed additives that can improve digestion and absorption of food improves poultry performance (5). Wheat has become an important energy source in poultry diets due to supply shortages and rising corn prices, even in markets that do not traditionally rely on wheat (6). ...
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In this study, effects of direct-fed microbial on qualitative traits of eggs and productive performance, ileal and cecal microflora and pH of hens fed wheat-based diets were investigated from 60 to 68 wk of age. The total number of 240 Hy-Line W-36 laying hens were randomly distributed between 40 cages, and the five experimental diets including (A) corn-soybean meal-based control diet, (B) wheat-based diet with no microbial additive, (C) wheat-based diet supplemented with Bacillus velezensis, (D) wheat-based diet supplemented with S. cerevisiae and (E) wheat-based diet supplemented with B. velezensis + S. cerevisiae were assigned to hens with 8 replicate cages per diet and 6 hens per each replicate. Increased egg weight and production (EP), feed intake (FI), improved feed conversion ratio (FCR) and decreased pH of ileum and caecum were observed in layers fed the diet included B. velezensis + S. cerevisiae (P<0.05). Improved intestinal morphology characteristics were observed in hens fed the microbe-added diets (P<0.05). Increased plasma protein, albumen and HDL in hens fed microbe-added diets (P<0.05). Adding B. velezensis + S. cerevisiae to diet, modulated the ileal and caecal microflora composition by decreasing the numbers of Salmonella and increasing the numbers of Lactobacilli. Based on the results of the current study, it can be concluded that adding B. velezensis + S. cerevisiae to the wheat-based diet improve performance and intestinal morphology characteristics of laying hens, decrease plasma levels of cholesterol, triglyceride, and LDL and the intestinal pH and number of Salmonella.