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Measurement printout demonstrating the height of spikes used for the calculation of the ratio: mean of internal spikes/L1.
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To determine repeatability and reproducibility of A-scan biometry quantitative findings in order to uniformly evaluate lenticular cataract changes.
Seventeen eyes from seventeen cataract patients were examined by three (trainee ophthalmologists). Results (ratio of mean internal lens spikes height/lens anterior surface spike height) were compared us...
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... parameters employed were mean height of the internal lens spikes and the height of the L1 spike. The ratio of these parameters was the main index of interest (mean of spikes/L1 ratio) and was calcu- lated using the program Image J 1.40 (Wayne Rasband, National Institutes of Health, Bethesda, MD, USA; Fig. ...
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Citations
... Currently, the ocular biometric methods that have been tested and reported in the literature include A-scan ultrasonography 13,22 , calipers and micrometers 14 , image analysis of histological sections 23 , MRI and CT scans 24 , PCI (also called optical low coherence interferometry (OLCI) 15 , and more recently, SD-OCT 11 . Techniques performed in vitro or ex-vivo (calipers, micrometers, histological sections) are associated with changes in eye length secondary to desiccation and loss of turgidity which compromise their reliability in detecting and measuring small changes in axial length (see 14 ). ...
To compare measurements of murine ocular axial lengths (ALs) made with 780 nm partial coherence interferometry (PCI) and 1310 nm spectral domain-optical coherence tomography (SD-OCT).
AL was measured at postnatal day (P) 58 in C57BL/6J mice. Repeated AL measurements were taken using a custom-made 780 nm PCI and a commercial 1310 nm SD-OCT. Intra- and interuser variability was assessed along the central optical axis and 2-degree off-axes angles with the SD-OCT. Data were collected and analyzed using Cronbach alpha (α), Bland-Altman coefficient of repeatability, agreement plots, and intraclass correlation coefficients (ICC).
AL measurements agreed well between the two instruments (3.262 ± 0.042 mm for PCI; 3.264 ± 0.047 mm for SD-OCT; n = 20 eyes). The ICC for PCI compared with SD-OCT was 0.92, confirming high agreement between the two instruments. Intrauser ICC for the PCI and SD-OCT were 0.814 and 0.995, respectively. Similarly, interuser ICC for PCI and SD-OCT were 0.970 and 0.943, respectively. Using SD-OCT, a 2-degree misalignment of the eye along the horizontal meridian produced mean differences in AL of -0.002 ± 0.017 mm relative to the centrally aligned images, whereas similar misalignment along the vertical meridian created 0.005 ± 0.018 mm differences in AL measurements.
AL measurements from the 780 nm PCI and 1310 nm SD-OCT correlate well. Multiple statistical indices indicate that both instruments have good precision and agreement for measuring murine ocular AL in vivo. Although the vertical meridian had the greater variability in AL in the small mouse eye; 2-degree off-axes differences were within the SD of centrally aligned AL.
