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Maximum parsimony tree based on combined ITS1-5.8S-ITS2 region and D1/D2 domains of the LSU rDNA sequences for Glaciozyma litorale sp. nov. The numbers given on branches are frequencies ([50 %) with which a given branch
Source publication
The intertidal yeast communities inhabiting various environments in the territories of the White Sea Biological Station "Kartesh" (WSBS ZIN RAS) and the N.A. Pertsov White Sea Biological Station (WSBS MSU) were studied. A total of 31 yeast species were isolated using a conventional plating technique and identified using molecular methods. The yeast...
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Ascophyllum nodosum, and to a lesser extent, Laminaria digitata, L. hyperborea and Fucus serratus, are marine algal species utilized in the commercial production of seaweed extracts used in agriculture. Betaines have been shown to be important constituents of these extracts, but there appears to have been no study made on whether there are variatio...
Citations
... Seven strains (VTT C-04510, VTT C-04552, VTT C-04553, VTT C-04554, VTT C-04555, VTT C-04556, and VTT C-04557) were isolated from an industrial malting ecosystem in Finland [26]. Strain 93b was isolated from the White Sea intertidal zone in Russia [27]. Strain 2EJM003 was isolated from an unknown source in China and strain CBS 11772 from plant Carex limosa in Russia. ...
... are from various sources (Table S1), viz. marine environment samples in China and Russia [27], swamp water in Korea [25], samples associated with industrial malting ecosystem in Finland [26], samples related to crop in USA and Germany [7], flower in Brazil and Korea [25], fruit in Hungray [29], and unknown source in Japan [28], suggesting the wide distribution of the new species in the world. The species in the genus Vishniacozyma have been found in various substrates, especially plant materials, including leaves and flowers. ...
Three strains belonging to the basidiomycetous yeast genus Vishniacozyma were isolated from marine water samples collected from intertidal zones in Liaoning province, northeast China. Phylogenetic analyses based on the sequences of the small subunit (SSU) ribosomal DNA (rDNA), the D1/D2 domain of the large subunit (LSU) ribosomal DNA (rDNA), the internal transcribed spacer region (ITS), the two subunits of DNA polymerase II ( RPB1 and RPB2 ), the translation elongation factor 1-α ( TEF1 ), and the mitochondrial gene cytochrome b ( CYTB ) showed that these strains together with 20 strains from various geographic and ecological origins from other regions of the world represent a novel species in the genus Vishniacozyma . We propose the name Vishniacozyma pseudocarnescens sp. nov. (holotype CGMCC 2.6457) for the new species, which differs phenotypically from its close relatives V. carnescens , V. tephrensis , and V. victoriae by its ability to grow at 30 °C and on 50 % (w/v) glucose-yeast extract agar.
... The blast results indicated that strain CGMCC 2.6672 T (original strain number 17-237 A-2) had a D1/D2 sequence that was similar to those of three members of the genus Cystofilobasidium, unidentified at the species level. These were KBP:Y-6672 from the gut of an insect (Thinoscatella quadrisetosa), K146b from an alga (Odonthalia dentate) collected in Russia and EX-F 1547 from basal ice collected in Norway [19,20]. Only one substitution and two indel differences were observed among these four strains in the D1/D2 domain. ...
A yeast strain belonging to the basidiomycetous yeast genus Cystofilobasidium was isolated from a marine sediment sample collected in an intertidal zone in Shandong province, PR China. The results of phylogenetic analyses based on sequences of the D1/D2 domain of the 26S ribosomal RNA gene and the internal transcribed spacer (ITS) region indicate that this strain, together with three other strains isolated from basal ice collected in Norway, the gut of an insect and an alga collected in Russia, represent a novel species of the genus, for which the name Cystofilobasidium josepaulonis sp. nov. (holotype strain CGMCC 2.6672T) is proposed. The novel species differs from the known species of the genus Cystofilobasidium by 1.7 %-4.1 and 11.3 %-17.1 % mismatches in the D1/D2 domain and the ITS region, respectively. This species forms teliospores on potato dextrose agar (PDA) and 10 % V8 juice agar, but teliospore germination with basidia was not observed.
... The species Sakaguchia dacryoidea alone can be, however, considered the primarily "marine" since its description was given from isolates from waters of the Indian, Pacific, and Southern oceans as well as strains from the Atlantic (data of the CBS and NCBI collections). Yeasts detected in our study on P. palmata were not reported before from the other White Sea macrophytes (Ascophyllum nodosum, Fucus vesiculosus, Odonthalia dentata, and Saccharina latissima), which may point to specificity of the yeast population forming on various algal species [29]. ...
This is a rst report about the diversity of micromycetes associated with living thalli of the edible red algae Palmaria palmata (dulse). We collected samples in the Kandalaksha Bay of the White Sea at upper (10 thalli in the littoral) and lower (10 thalli in the sublittoral) lines of this species distribution. The work was carried out by cultural methods, fungi were isolated from the surface of thalli. From one thallus from 0 to 55 colonies belongs to 0-24 morphotypes were isolated. The total diversity was 52 morphotypes, of which 48 were mycelia and 4 were yeast. All identi ed yeasts belong to Basidiomycota; among lamentous fungi, 2 species belongs to Zygomycota, and the rest belongs to Ascomycota, the vast majority of which are represented by anamorphic stages. The most diverse genera were Acremonium (10 morphotypes), Penicillium (9) and Cladosporium (6); the most numerous are Acremonium (158 colonies) and Cladosporium (103 colonies). Acremonium fuci is the most common species on the studied thalli (122 colonies on 15 thalli). The most important factor for the formation of the surface mycobiota of dulse is the habitat: littoral and sublittoral algal populations di er signicantly in the fungal communities number and structure. The thalli of the littoral population are much richer in fungi. The mycobiota of littoral samples in dominated by dark-colored species, as well as Acremonium fuci and Sarocladium strictum. While the mycobiota of sublittoral samples is dominated by lightcolored species, and most of the isolated yeasts were also founded here.
... The yeast genus Glaciozyma was proposed for the first time by Turchetti et al. [50], who described two new species (i.e., G. martinii and G. watsonii) and reclassified Leucosporidium antarcticum as G. antarctica. Due to its psychrophilic habit, Glaciozyma was found, but as minor fungal taxon, in both Arctic [51][52][53][54][55] and Antarctic sites [49,54,56,57]. The genome of G. antarctica has been recently sequenced and studied [46], reporting systems of psychrophilic responses apparently allowing yeast growth at very low (or even sub-zero) temperatures. ...
Rock glaciers are relatively common in Antarctic permafrost areas and could be considered postglacial cryogenic landforms. Although the extensive presence of rock glaciers, their chemical–physical and biotic composition remain scarce. Chemical–physical parameters and fungal community (by sequencing the ITS2 rDNA, Illumina MiSeq) parameters of a permafrost core were studied. The permafrost core, reaching a depth of 6.10 m, was divided into five units based on ice content. The five units (U1–U5) of the permafrost core exhibited several significant (p < 0.05) differences in terms of chemical and physical characteristics, and significant (p < 0.05) higher values of Ca, K, Li, Mg, Mn, S, and Sr were found in U5. Yeasts dominated on filamentous fungi in all the units of the permafrost core; additionally, Ascomycota was the prevalent phylum among filamentous forms, while Basidiomycota was the dominant phylum among yeasts. Surprisingly, in U5 the amplicon sequence variants (ASVs) assigned to the yeast genus Glaciozyma represented about two-thirds of the total reads. This result may be considered extremely rare in Antarctic yeast diversity, especially in permafrost habitats. Based on of the chemical–physical composition of the units, the dominance of Glaciozyma in the deepest unit was correlated with the elemental composition of the core.
... To date, 21 Cystobasidium species have been described, i.e., C. fimetarium, C. minutum, C. slooffiae, C. calyptogenae, C. pinicola, C. laryngis, C. benthicum, C. pallidum, C. lysinophilum, C. portillonensis, C. oligophagum, C. alpinum, C. psychroaquaticum, C. rietchieii, C. tubakii, C. ongulense, C. keelungensis [6][7][8][9][10], C. halotolerans [11], C. iriomotense C. raffinophilum and C. terricola [13]. Various habitats and diverse ecologies, i.e., supraglacial sediments [9], aquatic environments [10,[14][15][16][17][18], soil [8,[19][20][21] and phylloplanes [7,[22][23][24][25][26] have been associated with Cystobasidium yeasts. Previously classified in the Rhodotorula minuta clade [7], members of this genus have not been described as commensal organisms or human pathogens. ...
Background
Angular cheilitis, an infection mainly caused by Candida yeasts, is featured by the appearance of inflammatory lesions at the bilateral corners of the mouth, particularly in patients with poor oral hygiene, ill-fitting dentures and old age. The first isolation of an atypical yeast, Cystobasidium calyptogenae, from oral samples of a patient presenting with angular cheilitis is discussed in this study.
Case presentation
Angular cheilitis was diagnosed in a 60-year-old denture-wearing woman who presented with an irritation fibroma on her right lower buccal sulcus over the premolar region. Primary cultures of her oral swab and oral rinse samples grew a pure culture of an uncommon yeast strain resembling Rhodotorula sp. Sequence analysis of the yeast internal transcribed spacer (ITS) gene region and D1D2 domain showed highest similarity (99.6% and 100%, respectively) to C. calyptogenae CBS 9125 type strain. Following 2 weeks of treatment with miconazole/fusidic acid and mouthwash, the oral lesion showed improvement with less erythema. C. calyptogenae was not isolated from the patient’s oral samples upon repeat sampling.
Conclusion
This is the first report on the isolation of C. calyptogenae from human oral samples. The ability of C. calyptogenae to grow at 37 °C and the fact that it was the only yeast species isolated from the patient’s oral samples suggests its pathogenic potential and possible involvement in angular cheilitis. The ubiquitous nature of the Cystobasidium yeast is believed to increase the likelihood of opportunistic infections among immunocompromised individuals. As Cystobasidium is phenotypically indistinguishable from Rhodotorula, an emerging opportunistic pathogen, surveillance using molecular identification in clinical settings is essential in providing accurate diagnosis and treatment of uncommon yeast infections.
... We named these frozen colonies "frost-columnar colonies", and all these colonies were included in white yeasts. Five isolates of these yeasts among frost-columnar colonies were identified as G. antarctica since they were highly homologous to the ITS region and D1/D2 domains of G. antarctica CBS 5942 T (Figure S1), and the phylogenetic tree was a similar morphology as previously recorded [38]. G. antarctica was exclusively isolated from them and formed the same colonies in aseptic cultures of frozen PDA ( Figure 2C). ...
The basidiomycetous yeast, Glaciozyma antarctica, was isolated from various terrestrial materials collected from the Sôya coast, East Antarctica, and formed frost-columnar colonies on agar plates frozen at −1 °C. Thawed colonies were highly viscous, indicating that the yeast produced a large number of extracellular polysaccharides (EPS). G. antarctica was then cultured on frozen media containing red food coloring to observe the dynamics of solutes in unfrozen water; pigments accumulated in frozen yeast colonies, indicating that solutes were concentrated in unfrozen water of yeast colonies. Moreover, the yeast produced a small quantity of ice-binding proteins (IBPs) which inhibited ice crystal growth. Solutes in unfrozen water were considered to accumulate in the pore of frozen colonies. The extracellular IBPs may have held an unfrozen state of medium water after accumulation in the frost-columnar colony.
... Glaciozyma litorale, proposed by Kachalkin (2014), is not a validly published name (Shenzhen Code, Art. 35.1), as it was assigned to an invalidly published generic na me, Glaciozyma, based on G. antarctica (Fell, Statzell, I.L. Hunter & Phaff ) Turchetti et al. (Turchetti et al. 2011: 579). ...
Two generic names, Meira and Acaromyces, and nineteen species names of basidiomycetous yeasts, earlier proposed and invalidly published, are validated.
... psychrophenolica, P. psychrophila, P. glacialis [23]; Glaciozyma-G. watsonii, G.martinii, G. antarctica, G. litorale [24,25]; Camptobasidium -C. gelus [18]; and Cryolevonia-C. ...
Sampling campaigns in Greenland and Svalbard were executed to explore fungal diversity in cold habitats. Three very abundant groups of strains were discovered, consisting either of recently described or of yet-undescribed psychrophilic and oligotrophic yeasts and dimorphic fungi, accounting for around 50 % of the total cultivable diversity of basidiomycetes in our studies. The occurrence of these taxa has also been demonstrated by culture-independent methods. Based on phylogenetic analyses of ribosomal gene cluster sequences (D1/D2 domains of 28S (LSU), 18S (SSU), ITS with 5.8S rDNA) and sequences of protein-coding genes for elongation factor one alpha ( TEF ), cytochrome b ( CYTB ) and two subunits of the RNA polymerase II ( RPB1 and RPB2 ) obtained from pure cultures, the isolated taxa presented in this study belong to Basidiomycota, subphylum Pucciniomycotina, class Microbotryomycetes, family Camptobasidiaceae. The dataset of the sequences supported the recognition of three species: Camptobasidium gelus , Camptobasidium arcticum sp. nov. (ex-type strain EXF-12713) and Psychromyces glacialis gen. and sp. nov. (ex-type strain EXF-13111). Camptobasidium gelus was found in the Svalbard and Greenland samples, while representatives of the here proposed new species, C. arcticum , were found only in the Greenland Ice Sheet. Psychromyces gen. nov. was erected for the dimorphic/filamentous isolates found in Svalbard and Greenland glacial environments. The taxon, for which the invalid name ‘ Rhodotorula svalbardensis ’ has been used, belongs to this genus. Based on ribosomal genes, Camptobasidium arcticum and Psychromyces glacialis are related, phylogenetically most closely related to the genera Glaciozyma and Cryolevonia . Seven genes phylogeny restricted to taxa with available sequences, supported the placement of Psychromyces to Camptobasidiaceae.
... This new genus contains only one species, Cryolevonia schafbergensis, isolated in an ancient permafrost layer in the Alps and in melted sea ice in Bafn Island in Canada. The genus Camptobasidium comprises a single species, Camptobasidium hydrophilum, a slow-growing psychrophilic fungus without a yeast stage [2], whereas Glaciozyma contains four psychrophilic yeast species: G. antarctica, G. martinii, G. watsonii and G. litoralis [4,8]. In recent years, these four species, together with other psychrophilic yeasts are being extensively studied as a source of cold active proteins and enzymes of high biotechnological value [9][10][11]. ...
... In recent years, these four species, together with other psychrophilic yeasts are being extensively studied as a source of cold active proteins and enzymes of high biotechnological value [9][10][11]. Also, the strategies evolved by these taxa to cope with cold conditions are a matter of substantial investigation nowadays [8]. Hence, the description of novel psychrophilic Microbotryomycetes is important for improving the group taxonomy, and also for ecological and biotechnological fundamental and applied research. ...
Twenty-one psychrophilic yeast isolates related to the Camptobasidiaceae family in the Microbotryomycetes class were obtained from ice collected from cold environments worldwide. A new psychrophilic species from the recently described genus Cryolevonia, Cryolevania giraudoae is proposed to accommodate 18 isolates from Patagonia (Argentina) and Antarctica (holotype CRUB 2086T). In addition, a new psychrophilic species in the genus Camptobasidium is described as Camptobasidium gelus sp. nov. (holotype CBS 8941T), based on three isolates from glacial ice in the Russel glacier (Greenland ice sheet) and Antarctica. The strict psychrophilic profile is the salient feature of both novel species.
... These yeasts occurred also in associations with Drosophila and termites and were one of the dominant components of the yeast microbiome of the ants Formica aquilonia Yarrow, 1955 (family Formicidae) (Hoang et al., 2015;Maksimova et al., 2016;Stefanini, 2018). While this species occurs in the White Sea littoral biotopes, it is not predominant there (Kachalkin, 2014), although it is extremely common in other natural substrates at the Biological Station territory (Babjeva and Reshetova, 1998;Kachalkin et al., 2017). ...
... The species D. prunorum was isolated from the surface of garden plume fruits. While members of this genus were not previously found in the White Sea coastal biotopes (Kachalkin, 2014), they were found in the lichens at the Biological Station territory (Kachalkin et al., 2017). Symbiosis with insects is probably not common within this yeast genus (Stefanini, 2018). ...
... Symbiosis with insects is probably not common within this yeast genus (Stefanini, 2018). (Kachalkin, 2014). All these algal species were found in algae driven ashore where F. fucorum developed, including the sampling site at Cape Kuzokotskii. ...
