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Marasmius midnapurensis (holotype): A. Basidiomes. B. Siccus-type cells of pileipellis. C. Basidiospores. D. Basidium. E. Cheilocystidia. F. Caulocystidia. Scale bars: A = 10 mm; B, F = 10 µm; C-E = 5 µm.
Source publication
The marasmioid fungus Marasmius midnapurensis (Marasmiaceae, Basidiomycota) is described as a new species from India. Analysis of the internal transcribed spacer-1 region (ITS1) of the nuclear ribosomal RNA gene suggests that M. midnapurensis is phylogenetically
distinct from closely related species and confirms its position within Marasmius sect....
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Citations
... Genomic DNA was extracted as described in Dutta et al. (2014). ITS1 and ITS4 primers were used to amplify the entire nrITS region (White et al. 1990). ...
Two new species of Fomitopsidaceae, Pseudofomitopsis fusca R.Saha, A.K.Dutta & K.Acharya sp. nov. and Fomitopsis benghalensis R.Saha, A.K.Dutta & K.Acharya sp. nov., are described from West Bengal, India, based on morphological and molecular phylogenetic analyses (nuclear ITS sequence). Pseudofomitopsis fusca sp. nov. possesses perennial, triquetrous to ungulate, sessile basidiocarps with a shiny, glabrous, azonate, dark brown upper surface, a yellowish grey pore surface with angular pores (3–5 per mm), a dimitic type of hyphal system with clamped generative hyphae, fusoid cystidioles; ellipsoid, cotton blue positive, and basidiospores 3–5 × 1.5–3.5 µm. Fomitopsis benghalensis sp. nov. is characterized by its annual, resupinate basidiocarp with pilose, bluish white to orange-grey, warty, woody upper surface, bluish-white pore surface, circular to angular pores (5–7 per mm), a trimitic type of hyphal system with clamped generative hyphae, fusoid cystidioles, and cylindrical to elongate basidiospores (5.5–8 × 2.5–3.5 µm). The new taxa are compared to closely related taxa. Photomicrographs of the basidiocarps, along with detailed morphological descriptions and a molecular sequence-based phylogenetic tree, are provided.
... Phylogenetic protocols:-DNA extraction, PCR, and sequencing:-Genomic DNA was extracted following Dutta et al. (2014). ITS1 and ITS4 primers were used to amplify the entire nrITS region (White et al. 1990). ...
The present study deals with the descriptions of two Hirschioporaceae taxa from West Bengal, India, using morphological and molecular phylogenetic analyses of nrITS sequence data. Of these two taxa, Nigrohirschioporus violacaeruleum is newly described, while Pallidohirschiorus brastagii is a new record to West Bengal, India.
... During the past few years, repeated field investigations were conducted in various places in eastern India to unveil the species diversity of the genus Russula, and as a result, several new taxa have been discovered (Dutta et al., 2014;Khatua et al., 2015;Paloi et al., 2015Paloi et al., , 2016. The present manuscript deals with the description of a novel species of the Russula subg. ...
... The PCR cycle conditions used to amplify the target areas were those described by Dutta et al. (2014). The PCR product sequencing was outsourced to Agri Genome Labs Pvt. Ltd. in Kerala, India. ...
This paper describes a new Russula species, R . brunneoaurantiaca , from India with morphological and molecular sequence (nrITS) data, field pictures of basidiocarps, and comparisons with close relatives. Russula brunneoaurantiaca has a brownish orange pileus with a mucilaginous surface, sub‐decurrent lamellae that are white to pale orange, a white stipe that turns yellowish brown to brown when bruised, a strong, unpleasant smell, globose to subglobose basidiospores (5.0–9.0 5.0–7.8 m) with an inamyloid suprahilar spot and ornamentation of small isolated conical warts, fusiform hymenial cystidia on gill sides (62.5–82 × 7.5–12.5 μm) and lageniform to sub‐lageniform cystidia with filiform apex near the gill edge (80–113 × 7.5–10 μm), fusiform to spindle‐shaped pileocystidia, and habitat in association with Castanopsis sp. A complete morphological description, photographs, and molecular sequence‐based phylogenetic trees demarcating the position of the novel taxon are provided.
Research Highlights
Scanning electron microscopy (SEM) and subsequent DNA analysis revealed a new species of the genus Russula .
SEM analysis is an additional technique to describe the size and shape of its basidiospores as well as their ornamentation.
The diagnostic characteristics, habit, habitat, and similarities to related species are given.
... D N A e x t r a c t i o n , P C R a n d s e q u e n c i n g. Genomic DNA was extracted as described in Dutta et al. (2014). ITS1 and ITS4 primers were applied to amplify the entire nrITS region (White et al. 1990). ...
Murinicarpus subadustus described from China is the only species of the genus Murinicarpus (Polyporales, Basidiomycota). There is no other report of this genus from any other country except China. Here, we report M. subadustus as a novelty to the macrofungal biota of India. This unique species is identified based on morphological features and nrDNA ITS-based phylogenetic analysis. A thorough macro- and microscopic characterisation along with field photographs, line drawings of microscopic structures and comparisons with morphologically and phylogenetically related taxa are provided.
... Microchemical tests were carried out with MR, KOH 5%, lactophenol cotton blue (CB) 1%, and ammonium hydroxide (NH4OH) 10% for mycelium; and MR, CB 1% and NH4OH 10% for spores (Dwivedi et al. 2012;Mohanan 2014). Macrofungi were identified from the standard literature and the description of legitimate current name status registered in Mycobank database (Gäumann 1928;Thomas 1928;Corner 1950;Cooke 1961;Denison 1963;Dennis 1978;Dutta et al. 2014;Dwidjoseputro 1978;Dring 1980;Arora 1986;Gilbertson and Ryvarden 1986;Largent 1986;Singer 1986;Hjortstam et al. 1987;Miller and Miller 1988;Largent et al. 1997;Pegler et al. 1997;Spooner 2000;Lodge 2004;Deacon 2005;Halling and Mueller 2005;Cannon and Kirk 2007;Parfitt et al. 2007;Kirk et al. 2008;Laessøe 2013;Petersen 2013;Beug et al. 2014;Shay et al. 2017;Wu et al. 2017;Hussain et al. 2018;Laessøe and Petersen 2019a, b). ...
Arko PF, Sudirman LI, Qayim I. 2021. Changes in landscape ecology between nature reserve and palm oil plantation in West Java, Indonesia based on the observations of macrofungal population. Biodiversitas 22: 4526-4537. As the biggest tropical country in Southeast Asia with the third-largest forest, Indonesia has limited data on macrofungal diversity. The limitation of the data is due to the lack of study on macrofungi in high biodiversity locations such as Dungus Iwul Nature Reserve (CADI). The purpose of this study was to determine and analyze the population of macrofungal species caused by the conversion in landscape structure (fragmentation and land-use change) that occurred in CADI patch and oil palm plantation of PT Perkebunan Nusantara VIII (PTPN) matrix, Cigelung, West Java. The sampling method used was opportunistic sampling assisted by line intercept. The macrofungal identification was based on morphological characters. The analysis of community ecology was performed in R programme. The results showed that macrofungal population and diversity in CADI were the highest, followed by PTPN planted in 2004 and planted in 2003, with a significant difference among the three study locations. The community dominance index shows the opposite, with the highest in PTPN 2003 and the lowest in CADI. These results showed changes in macrofungal population and diversity from the conversion in landscape structure. There were eleven indicator species in CADI, two species in PTPN 2003, and five species in PTPN 2004.
... So far, about 85 species (Bilgrami et al. 1979;Manjula 1983;Farook et al. 2013;Dutta et al. 2014Dutta et al. , 2015Farook & Manimohan 2015;Dutta & Acharya 2018) of Marasmius sensu lato have been reported from India. From Kerala State, 25 species have been recorded so far (Farook et al. 2013;Farook & Manimohan 2015). ...
... Marasmius midnapurensis, a species recently described from India (Dutta et al. 2014), has light yellowish brown patches at the center of the pileus, larger basidiospores (10.7-15 × 3.5-4.7 μm), interwoven lamellar trama, lamellae devoid of pleurocystidia and Siccus-type broom cells in the stipitipellis. ...
Marasmius cystidiatus sp. nov. is described from Kerala State, India based on both morphology and nrITS sequence-based molecular phylogeny. The phylogenetic tree inferred from maximum likelihood analysis showed M. cystidiatus nesting within the clade representing Marasmius section Globulares. Within this clade, M. cystidiatus formed a distinct lineage, sister to a cluster containing mostly members of the series Atrorubentes. A comprehensive description and photographs of both basidiocarps and microscopic structures of the new species are provided along with a comparison with morphologically close and phylogenetically related species.
... 2 In the previous years, our research team has conducted extensive field works and recorded an immense number of wild edible mushrooms from different corners of the state with the help of tribal and ethnic forest dwellers of the regions who prefer those as their daily square meal. 3 Taxonomic and molecular exploration revealed that many of them are new to science, 4,5 new to the record for India 6,7 and an addition to the macrofungal flora of West Bengal. 8,9 Throughout the globe, mushrooms are well known to the human civilizations because of having nutritional, culinary values, and medicinal prospects. ...
Objectives:
Oudemansiella canarii is an edible mushroom highly appreciated throughout the world due to its being a gastronomic delicacy. To date, no extensive work has been reported on the pharmacological or antioxidative aspects of this macrofungus. The present study focuses on the micromorphological features, confirmation of its identity based on molecular sequence (nrITS rDNA) data, and determination of its physicochemical parameters such as organoleptic features and fluorescent behavior.
Materials and methods:
Collected basidiocarps were powdered and used for microscopic and organoleptic evaluation. 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method, total antioxidant activity methods, and 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay were used for evaluating the antioxidant capacities of the methanolic extract. High-performance liquid chromatography (HPLC) analysis profile was also recorded to analyze the phenolic fingerprint.
Results:
The DPPH radical scavenging activity was determined with an EC50 value of 0.912 μg, total antioxidant activity was found to be 15.33 μg ascorbic acid equivalent/mg of extract, and the ABTS assay revealed 12.91 μm TE/mg of extract antioxidant activity. The HPLC chromatogram revealed the presence of 12 peaks. Several parameters were tested for the determination of chemical composition, revealing the existence of major bioactive components in the extract in the following order: phenol>flavonoid>ascorbic acid>β-carotene~lycopene.
Conclusion:
The present work suggests that O. canarii may be considered a novel prospect as a functional food and antioxidant supplement.
... During last sixteen years, our laboratory has surveyed different areas of West Bengal and collected interesting basidiomes of Agaricales and many of which have already been reported [8][9][10][11][12][13][14][15][16][17][18][19][20] . The present paper deals with the new records of five macromycetes belonging to various families under Agaricales from West Bengal. ...
Ongoing studies have suggested that there is enormous macrofungal diversity in West Bengal due to its unique geo-climatic setting. The present paper reports five macrofungi viz. Cortinarius iodes, Xerula radicata, Phylloporus bellus, Crepidotus mollis and Macrocybe gigantea collected from different geographical regions of West Bengal, India and are reported herein with detailed macro-morphological characters.
... During last sixteen years, our laboratory has surveyed different areas of West Bengal and collected interesting basidiomes of Agaricales and many of which have already been reported [8][9][10][11][12][13][14][15][16][17][18][19][20] . The present paper deals with the new records of five macromycetes belonging to various families under Agaricales from West Bengal. ...
Five members of Agaricales (Agrocybe pediades, Calocybella swetha, Cantharocybe virosa, Lepiota echinacea, and Oudemansiella canarii) were collected from different parts of West Bengal, India and are reported herein with detailed morphological characters. Literature survey revealed that all of the collected taxa represent their first distributional record from the state.
http://rjptonline.org/AbstractView.aspx?PID=2017-10-3-33
... The state West Bengal is geoclimatically exclusive and diverse, being home of the Great Himalayan mountain range, extensive coastal and Gangetic plains, barren and extreme plateaus, which provides a niche for an unfathomable number of macrofungi to flourish (Dutta et al. 2015a, b). Thus, several new taxa belonging to a number of agaric genera have been described in recent times from the region by a number of researchers (Dutta et al. 2014, 2015a, b, Das et al. 2015, Paloi et al. 2015, 2016. ...
... DNa extractioN, pcr aND cycle sequeNciNg: Genomic DNA was extracted following Dutta et al. (2014). The entire nrDNA ITS region (ITS1, 5.8S, and ITS2) was amplified on Applied Biosystems® 2720 automated thermal cycler using primer pair ITS1 and ITS4 (White et al. 1990). ...
Clitocybula albida is described from West Bengal, India. A comprehensive description, photographs, and comparisons with morphologically similar and phylogenetically related species are provided. Molecular phylogenetic analyses based on ITS sequences coupled with morphology confirmed the placement of this species in Clitocybula. © 2017 J. Cramer in Gebr. Borntraeger Verlagsbuchhandlung, Stuttgart, Germany.
