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Source publication
The aim of this study is to determine seasonal changes in the blood levels of some heavy metals in three Turkey-specific cat-tle breed: Native Land (NL), Grizzle Race (GR), and South Anatolian Red (SR). Blood samples were collected in summer and winter seasons from a total of 180 cattle aged 12–24months. The levels of the essential (Zn, Cu, Fe, Co...
Context in source publication
Context 1
... study protocol was approved by the Kırıkkale University Ethics Committee for Animal Experiments (Decision No: 2014/79). The blood samples of the SR, NL, and GR races were collected from the Adana, Çorum, and Edirne regions, respectively. A map (Fig. 1) was included in the manuscript to visualize the locations of the each city cited. Blood samples taken from a total of 180 cattle were collected during the summer and winter season of 2015. Blood samples were transferred to the Kırıkkale University Core Laboratory Facility in the cold chain. In the laboratory, blood samples were ...
Citations
... The vast majority of these studies have focused on the dose-dependent effects of stimulating metabolic processes and animal productivity [3]. Moreover, parameters such as breed, age, gender, physiological state [4], production technology [5], antagonism between elements [6], sanitary and hygienic conditions of keeping, and environmental quality [7,8] can significantly influence the need and accumulation of these elements in animal tissues. ...
Background and Aim
At present, detailed studies are being conducted to confirm the safety of the use of metal-containing ultrafine particles (UFP) in animal feeding, preventing the possibility of negative effects on productive qualities and physiological state, as well as on the environment and final consumer, that is, humans. Thus, the purpose of this research was to study the safety of cobalt- and manganese-containing UFP (UFP Co3O4, Mn2O3 UFP) together with Origanum vulgare (PB) herb extract in a bioluminescence inhibition test, as well as the effect of this composition on ruminal digestion in vitro.
Materials and Methods
The safety of the studied samples was determined using a multifunctional microplate analyzer TECAN Infinite F200 (Tecan Austria GmbH, Austria), recording the luminescence value of the bacterial strain Escherichia coli K12 TG11 (Ecolum, JSC NVO Immunotech, Russia). Dry matter (DM) digestibility studies were performed using the in vitro method on an “artificial rumen” model using an ANKOM Daisy II incubator unit (AD II; USA). The number of protozoa in ruminal fluid was counted in a Goryaev chamber. The bacterial mass was assessed by differential centrifugation followed by drying. This method is based on differences in the sedimentation rate of particles that differ in size and density.
Results
UFP Co3O4 and Mn2O3 at concentrations above 1.5 × 10⁻⁵ and 1.9 × 10⁻³ mol/L, respectively, have a pronounced bactericidal effect, suppressing more than 50% of the luminescence of E. coli K12 TG1. The combined use of UFP metals and plant extract increases the luminescence of the test object, indicating its safety. The combined use of UFP and PB increases the digestibility of feed DM in vitro and the number of protozoa in 1 mL of ruminal fluid; however, the combination of UFP Mn2O3 + PB (13.8%) yielded the best result, which is recommended for further in vivo research.
Conclusion
Origanum vulgare extract reduces the toxicity of UFP Co3O4 and Mn2O3 in vitro, indicating that their combined use is safer.
... The metal concentrations measurement: Algae samples were digested in a microwave oven (CemMars6) and were determined by optical emission Spectrometry (ICP-OES, SpectroBlue, Germany) for Cu and Pb metals (Aluc & Ekici, 2019). Each experiment was repeated three times, and the average was taken. ...
The aim of this work was to determine the content of lead (Pb), chromium (Cr), cadmium (Cd), arsenic (As), copper (Cu), strontium (Sr) and thallio (Tl) in blood, milk and urine from cows fed forage irrigated with sewage water and cultivated in the vicinity of the Popocatépetl volcano and determine the rate of transfer of these elements from blood to milk and urine. Digestion was performed in a microwave oven (CEM-MarsX). The determination was made using an ICP -OES. The Cd in the blood was significantly higher than in milk and urine. The Cr in the blood was higher than in the urine. In milk the content of Pb and Cu was significantly higher than in blood. Sr and Tl concentration in the urine was higher than that detected in milk and blood and Pb in blood. Milk transfer Index revealed a significant difference in relation to the TI values found in the urine and only in the case of Cu in milk was TI > 1. In urine, the values found of Sr and Tl were higher than milk TI. It is concluded that when metals are ingested by cows in fodder, they are easily transferred to milk and urine through the blood. Presence of heavy metals in the different matrices determines the degree of environmental and trofic chain contamination with which we can consider the cow a biomarker.
