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– Magnicamarosporium diospyricola (MFLU 17-0001, holotype). a Appearance of conidiomata on host surface. b Close-up of conidioma on host surface. c Vertical section of conidioma. d Ostiole part. e Pycnidial walls. f Paraphyses. g–j Developing stages of conidia from conidiogenous cells. k–o Developing stages of conidia. p Germinated conidia. q–r Culture characters on MEA. Scale bars: b = 500 µm, c = 200 µm, d = 100 µm, e = 50 µm, f–o = 20 µm.
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A new species of Magnicamarosporium, M. diospyricola was found on dead or dying twigs of a dicotyledonous plant in southern Thailand. The new species is distinct from other species in Sulcatisporaceae, as it has dematiaceous dictyosporous conidia. It differs from Magnicamarosporium iriomotense in its smaller conidiomata and conidia. Bayesian infere...
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... Phylogram generated from maximum likelihood analysis is based on combined LSU, SSU, ITS, and tef1-α sequence data. Related sequences of Sulcatisporaceae were obtained from Phukhamsakda et al. [35,36]. ML bootstrap values equal to or greater than 75% and Bayesian posterior probabilities (BYPP) equal to or greater than 0.95 are indicated above the branches. ...
This paper highlights the taxonomy of some interesting saprobic microfungi associated with dead plant materials of Hedychium coronarium, Lilium longiflorum, and Magnolia species. The taxa reported in this study belong to the orders Pleosporales and Kirschsteiniotheliales (Dothideomycetes). These taxa were identified based on multi-locus phylogeny of nuclear ribosomal DNA (rDNA) (LSU, SSU, and ITS) and protein-coding genes (tef1-α and rpb2), together with comprehensive morphological characterization. Two novel saprobic species, Leptoparies magnoliae sp. nov. and Neobambusicola magnoliae sp. nov., are introduced from Magnolia species in Thailand. Another new species, Asymmetrispora zingiberacearum sp. nov., is also described from dead stems of H. coronarium, which is the first asexual morph species of the genus Asymmetrispora. In addition, Ramusculicola thailandica and Kirschsteiniothelia thailandica are reported as new host records from dead twigs of Magnolia species. Sphaerellopsis paraphysata is reported as a new host record from L. longiflorum. Newly described taxa are compared with other similar species and detailed descriptions, micrographs, and phylogenetic trees to show the positions are provided.
... The species in this family are mostly restricted to terrestrial habitats excluding Neobambusicola strelitziae, which was found from the coastal region (Eastern Cape Province) of South Africa [58]. The conidia of anamorphs of Sulcatisporaceae can vary from hyaline, aseptate or septate (Anthosulcatispora, Loculosulcatispora, Neobambusicola, Pseudobambusicola), to pigmented phragmo-conidia (Sulcatispora) or muriform conidia (Magnicamarosporium), with or without striation [36,46,56,[58][59][60]. The anamorph of Parasulcatispora is yet to be discovered. ...
In the course of investigating the systematics of woody litter micromycete associates in Yunnan Province, China, we found one new species in Phaeoseptaceae, one new genus and three new species in Sulcatisporaceae from 16 specimens collected (ten collections of ascomycetous teleomorphs, four collections of hyphomycetous and two collections of coelomycetes anamorphs) from Ailaoshan, Chuxiong, Diqing, Honghe, Kunming, Lancang, Mengla and Yuxi in Yunnan Province. These taxonomic novelties were recognized with the aid of morphological comparisons and phylogenetic analyses of multiple gene sequences (non-translated loci and protein-coding regions). Pleopunctum menglaense sp. nov. is accommodated in Phaeoseptaceae (Pleosporales) based on its hyphomycetous anamorph, which is characterized by superficial sporodochia on the host surface, macronematous, mononematous, cylindrical, unbranched, aseptate, hyaline and smooth-walled conidiophores, monoblastic, terminal, hyaline conidiogenous cells, hyaline, muriform α conidia, and brown, muriform β conidia with tri-lobed wing like basal cells. Kazuakitanaka gen. nov. (type: K. yuxiensis) is introduced in Sulcatisporaceae (Massarineae, Pleosporales) for a saprobic ascomycete with teleomorphic and anamorphic (coelomycetous) features. The teleomorph possesses globose to subglobose ascomata with acentric ostiole, a peridial wall of textura angularis to textura prismatica, cylindric-clavate, pedicellate asci with an ocular chamber, and 1–2-septate, hyaline, fusiform, guttulate ascospores with a distinct mucilaginous sheath. The anamorph features pycnidial conidiomata, phialidic, ampulliform to cylindrical, hyaline conidiogenous cells and ampulliform to cylindrical, one-to-three-septate, hyaline, guttulate conidia. Loculosulcatispora was known only from its anamorph of L. thailandica. We observed the teleomorph of Loculosulcatispora hongheensis sp. nov. and amended the generic description of Loculosulcatispora accordingly. Loculosulcatispora hongheensis is characterized by globose to subglobose ascomata with a central ostiole, a peridial wall of textura angularis to globosa, branched, septate, pseudoparaphyses, clavate asci with a short pedicel and a minute ocular chamber and hyaline, fusiform, 1-septate ascospores with a thick irregular mucilaginous sheath. This study provides some insights into the diversity of fungi on dead woody litter in terrestrial habitats.
... Sulcatisporaceae is characterized by immersed to erumpent, subglobose to hemisphaerical ascomata, short ostiolar neck, trabecular pseudoparaphyses (Liew et al. 2000), clavate, short pedicellate asci, and broadly fusiform, hyaline, septate ascospores with mucilaginous appendages . Asexual morph is pycnidial conidiomata with various conidia characters Phukhamsakda et al. 2017a;Rupcic et al. 2018). We provide an updated tree of Sulcatisporaceae based on the combined dataset of LSU, ITS, SSU and tef1 sequence data and propose two new genera from Clematis species collected in Thailand. ...
... Thick branches represent significant support values from all analyses (BS ≥ 70%/BYPP ≥ 0.95) 2019). Asexual morphs in Sulcatisporaceae comprised Magnicamarosporium, Neobambusicola, Pseudobambusicola and Sulcatispora Phukhamsakda et al. 2017a;Rupcic et al. 2018). Anthosulcatispora is distinct within Sulcatisporaceae in having brown ascospores, while the sexual morph in Sulcatispora has hyaline, broadly fusiform ascospores with an entire sheath . ...
The cosmopolitan plant genus Clematis contains many climbing species that can be found worldwide. The genus occurs in the wild and is grown commercially for horticulture. Microfungi on Clematis were collected from Belgium, China, Italy, Thailand and the UK. They are characterized by morphology and analyses of gene sequence data using an integrated species concept to validate identifications. The study revealed two new families, 12 new genera, 50 new species, 26 new host records with one dimorphic character report, and ten species are transferred to other genera. The new families revealed by multigene phylogeny are Longiostiolaceae and Pseudomassarinaceae in Pleosporales (Dothideomycetes). New genera are Anthodidymella (Didymellaceae), Anthosulcatispora and Parasulcatispora (Sulcatisporaceae), Fusiformispora (Amniculicolaceae), Longispora (Phaeosphaeriaceae), Neobyssosphaeria (Melanommataceae), Neoleptosporella (Chaetosphaeriales, genera incertae sedis), Neostictis (Stictidaceae), Pseudohelminthosporium (Neomassarinaceae), Pseudomassarina (Pseudomassarinaceae), Sclerenchymomyces (Leptosphaeriaceae) and Xenoplectosphaerella (Plectosphaerellaceae). The newly described species are Alloleptosphaeria clematidis, Anthodidymella ranunculacearum, Anthosulcatispora subglobosa, Aquadictyospora clematidis, Brunneofusispora clematidis, Chaetosphaeronema clematidicola, C. clematidis, Chromolaenicola clematidis, Diaporthe clematidina, Dictyocheirospora clematidis, Distoseptispora clematidis, Floricola clematidis, Fusiformispora clematidis, Hermatomyces clematidis, Leptospora clematidis, Longispora clematidis, Massariosphaeria clematidis, Melomastia clematidis, M. fulvicomae, Neobyssosphaeria clematidis, Neoleptosporella clematidis, Neoroussoella clematidis, N. fulvicomae, Neostictis nigricans, Neovaginatispora clematidis, Parasulcatispora clematidis, Parathyridaria clematidis, P. serratifoliae, P. virginianae, Periconia verrucose, Phomatospora uniseriata, Pleopunctum clematidis, Pseudocapulatispora clematidis, Pseudocoleophoma clematidis, Pseudohelminthosporium clematidis, Pseudolophiostoma chiangraiense, P. clematidis, Pseudomassarina clematidis, Ramusculicola clematidis, Sarocladium clematidis, Sclerenchymomyces clematidis, Sigarispora clematidicola, S. clematidis, S. montanae, Sordaria clematidis, Stemphylium clematidis, Wojnowiciella clematidis, Xenodidymella clematidis, Xenomassariosphaeria clematidis and Xenoplectosphaerella clematidis. The following fungi are recorded on Clematis species for the first time: Angustimassarina rosarum, Dendryphion europaeum, Dermatiopleospora mariae, Diaporthe ravennica, D. rudis, Dichotomopilus ramosissimum, Dictyocheirospora xishuangbannaensis, Didymosphaeria rubi-ulmifolii, Fitzroyomyces cyperacearum, Fusarium celtidicola, Leptospora thailandica, Memnoniella oblongispora, Neodidymelliopsis longicolla, Neoeutypella baoshanensis, Neoroussoella heveae, Nigrograna chromolaenae, N. obliqua, Pestalotiopsis verruculosa, Pseudoberkleasmium chiangmaiense, Pseudoophiobolus rosae, Pseudoroussoella chromolaenae, P. elaeicola, Ramusculicola thailandica, Stemphylium vesicarium and Torula chromolaenae. The new combinations are Anthodidymella clematidis (≡ Didymella clematidis), A. vitalbina (≡ Didymella vitalbina), Anthosulcatispora brunnea (≡ Neobambusicola brunnea), Fuscohypha kunmingensis (≡ Plectosphaerella kunmingensis), Magnibotryascoma rubriostiolata (≡ Teichospora rubriostiolata), Pararoussoella mangrovei (≡ Roussoella mangrovei), Pseudoneoconiothyrium euonymi (≡ Roussoella euonymi), Sclerenchymomyces jonesii (≡ Neoleptosphaeria jonesii), Stemphylium rosae (≡ Pleospora rosae), and S. rosae-caninae (≡ Pleospora rosae-caninae). The microfungi on Clematis is distributed in several classes of Ascomycota. The analyses are based on morphological examination of specimens, coupled with phylogenetic sequence data. To the best of our knowledge, the consolidated species concept approach is recommended in validating species.
... Thailand is a highly biodiverse country in the tropics with hot and humid climate (MacKinnon et al. 1986, Marod andKutintara 2012). Although the fungal diversity in Thailand has been relatively well-studied (Rostrup 1902, Schumacher 1982, Hyde 1989, Jones 2000, Jones et al. 2006, Suetrong et al. 2009), the number of species being discovered is steadily growing due to increasing activities in studying microfungi in a large variety of terrestrial and aquatic ecosystems (Mapook et al. 2016, Dai et al. 2017, Doilom et al. 2017, Phukhamsakda et al. 2017. ...
The monotypic genus Neoaquastroma (Parabambusicolaceae, Pleosporales) was introduced for a micro-fungus isolated from a collection of dried stems of a dicotyledonous plant in Thailand. In this paper, we introduce two novel species, N. bauhiniae and N. krabiense, in this genus. Their asexual morphs comprise conidiomata with aseptate and hyaline conidia. Neoaquastroma bauhiniae has ascomata, asci and ascospores that are smaller than those of N. krabiense. Descriptions and illustrations of N. bauhiniae and N. krabiense are provided and the two species compared with the type species of the genus, N. guttulatum. Evidence for the introduction of the new taxa is also provided from phylogenetic analysis of a combined dataset of partial LSU, SSU, ITS and tef1 sequence data. The phylogenetic analysis revealed a distinct lineage for N. bauhiniae and N. krabiense within the family Parabambusicolaceae.
This is the ninth in a series of Mycosphere notes wherein we provide notes on various fungal taxa in Dothideomycetes. In this set of notes, we focus on species associated with woody oil plants, which are important in terms of food security, ecology, agriculture and for industrial manufacturing. Eighty-five fungal taxa were identified in three orders (Muyocopronales, Pleosporales and Valsariales). Thirty-four species (belonging to 16 families) were identified in Pleosporales, one species was classified in Valsariales and one species was recognized in Muyocopronales. We introduce two new genera Oleaginea (Lophiostomataceae) and Neobrevicollum (Neohendersoniaceae) and 16 new Valsaria insitiva. Three new asexual/sexual morph connections were made for Acrocalymma pterocarpi, Austropleospora ochracea and Loculosulcatispora hongheensis. A morphological description of one known species, Nigrograna locuta-pollinis, is provided. All taxa are described and illustrated, and phylogenetic trees are presented to show their phylogenetic placements.
Fungi are vital functional members of the biosphere, playing a crucial role in sustaining ecosystems by maintaining the nutrient balance. Many studies have verified the abundance of fungi across all-natural ecosystems and habitats, such as in forests, fresh-water (including both lentic or lotic), marine environments and deserts. With the focus previously on temperate regions and to a lesser extent biodiversity hotspots, the fungi in other areas remain overlooked. Therefore, it is imperative for mycologists to focus on taxa from these less-studied habitats, those dwelling on a vast number of hosts, and fungi that co-exist with other life forms. Molecular tools have been vital for species identification, in phylogeny, and linking sexual and asexual morphs. Identification of taxa based on the phylogenetic species concept, which relies on multiple loci and concordance of more than one gene genealogy, reduces subjectivity when determining the limits of a phylogenetic species. Large numbers of fungi inhabit biodiversity hotspots; however, they are underexplored owing to the vast diversity present and lack of studies. As examples of illustrating the undiscovered asexual fungi, this paper reports one new genus (Uniappendiculata Tibpromma), six new species (Caprettia lichexanthotricha Aptroot & M.F. Souza, Hermatomyces maharashtraense Rajeshkumar et al., Lichenoconium hawksworthii Flakus et al., Phaeobotryon spiraeae L.X. Zhang & X.L. Fan, Rachicladosporium aridum L. Selbmann & C. Coleine and Uniappendiculata kunmingensis Tibpromma) and one new host and country record (Apiculospora spartii Wijayaw. et al.). The paper discusses the biodiversity rich areas of South-Western China, South America and India, lessstudied habitats (rock inhabiting fungi, lichens with conidiomata and lichenicolous fungi), and geographically widespread, but lesser studied hosts to show substantial studies are needed to reveal the extent of fungal diversity. The impact of discovering cryptic species on cataloguing fungal species numbers is also discussed. Each section exemplifies the status of the current research in that genus and future work that is needed.
This article is the ninth in the series of Fungal Diversity Notes, where 107 taxa distributed in three phyla, nine classes, 31 orders and 57 families are described and illustrated. Taxa described in the present study include 12 new genera, 74 new species, three new combinations, two reference specimens, a re-circumscription of the epitype, and 15 records of sexual-asexual morph connections, new hosts and new geographical distributions. Twelve new genera comprise Brunneofusispora, Brunneomurispora, Liua, Lonicericola, Neoeutypella, Paratrimmatostroma, Parazalerion, Proliferophorum, Pseudoastrosphaeriellopsis, Septomelanconiella, Velebitea and Vicosamyces. Seventy-four new species are Agaricus memnonius, A. langensis, Aleurodiscus patagonicus, Amanita flavoalba, A. subtropicana, Amphisphaeria mangrovei, Baorangia major, Bartalinia kunmingensis, Brunneofusispora sinensis, Brunneomurispora lonicerae, Capronia camelliae-yunnanensis, Clavulina thindii, Coniochaeta simbalensis, Conlarium thailandense, Coprinus trigonosporus, Liua muriformis, Cyphellophora filicis, Cytospora ulmicola, Dacrymyces invisibilis, Dictyocheirospora metroxylonis, Distoseptispora thysanolaenae, Emericellopsis koreana, Galiicola baoshanensis, Hygrocybe lucida, Hypoxylon teeravasati, Hyweljonesia indica, Keissleriella caraganae, Lactarius olivaceopallidus, Lactifluus midnapurensis, Lembosia brigadeirensis, Leptosphaeria urticae, Lonicericola hyaloseptispora, Lophiotrema mucilaginosis, Marasmiellus bicoloripes, Marasmius indojasminodorus, Micropeltis phetchaburiensis, Mucor orantomantidis, Murilentithecium lonicerae, Neobambusicola brunnea, Neoeutypella baoshanensis, Neoroussoella heveae, Neosetophoma lonicerae, Ophiobolus malleolus, Parabambusicola thysanolaenae, Paratrimmatostroma kunmingensis, Parazalerion indica, Penicillium dokdoense, Peroneutypa mangrovei, Phaeosphaeria cycadis, Phanerochaete australosanguinea, Plectosphaerella kunmingensis, Plenodomus artemisiae, P. lijiangensis, Proliferophorum thailandicum, Pseudoastrosphaeriellopsis kaveriana, Pseudohelicomyces menglunicus, Pseudoplagiostoma mangiferae, Robillarda mangiferae, Roussoella elaeicola, Russula choptae, R. uttarakhandia, Septomelanconiella thailandica, Spencermartinsia acericola, Sphaerellopsis isthmospora, Thozetella lithocarpi, Trechispora echinospora, Tremellochaete atlantica, Trichoderma koreanum, T. pinicola, T. rugulosum, Velebitea chrysotexta, Vicosamyces venturisporus, Wojnowiciella kunmingensis and Zopfiella indica. Three new combinations are Baorangia rufomaculata, Lanmaoa pallidorosea and Wojnowiciella rosicola. The reference specimens of Canalisporium kenyense and Tamsiniella labiosa are designated. The epitype of Sarcopeziza sicula is re-circumscribed based on cyto- and histochemical analyses. The sexual-asexual morph connection of Plenodomus sinensis is reported from ferns and Cirsium for the first time. In addition, the new host records and country records are Amanita altipes, A. melleialba, Amarenomyces dactylidis, Chaetosphaeria panamensis, Coniella vitis, Coprinopsis kubickae, Dothiorella sarmentorum, Leptobacillium leptobactrum var. calidus, Muyocopron lithocarpi, Neoroussoella solani, Periconia cortaderiae, Phragmocamarosporium hederae, Sphaerellopsis paraphysata and Sphaeropsis eucalypticola.
