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The coagulase-negative staphylococci (CoNS) have long been considered to be low pathogenicity. The possibility of a horizontal transfer of resistance and virulence genes from S. aureus to CoNS could increase the pathogenicity of these bacteria. The objective of this work is to contribute to a better knowledge of the pathogenicity of (CoNS) strains...
Contexts in source publication
Context 1
... detection of the mecA gene in methicillin-resistant CoNS (MR-CoNS) strains was performed by monoplex PCR using specific primers (Table 1) tial denaturation at 94˚C for 3 min followed by 36 cycles of denaturation at 94˚C ...Context 2
... typing of CCSmec present in CoNSRM was done via multiplex PCR using specific primer pairs (Table 1) capable of detecting CCSmec type I, II and III. ...Context 3
... sequences of the different primers used are shown in Table 1. The reaction mixture consisted of 2 µL of buffer, 0.1 µL of Taq polymerase at a final concentration of 0.025 units/µL, 0.4 µL of dNTP, 1 µL at a final concentration of 10 µM of the sense and antisense primers of each of the three primer pairs, 5 µL of DNA and 6.5 µL of sterile distilled water for a final volume of 20 µL. ...Similar publications
Background: Methicillin-resistant Staphylococcus aureus (MRSA) is a group of S. aureus strains containing the SCCmec gene causing beta-lactam antibiotic resistance. MRSA is common in healthcare settings and can cause serious problems. Objective: The study aimed to investigate the prevalence of MRSA nasal colonization among privates of the Medical P...
Citations
Introduction: Beninese traditional used of several plants in the treatment of sickle cell disease. The safety of many of these plants did not be established and Daniellia Oliveri is one of them. To counter this, the present work aimed to determine the safety of the aqueous extract of Daniellia Oliveri on female rats of wistar strains. Methods: The aqueous extract of the barks of Daniellia Oliveri was obtained by maceration. The Acute Oral Toxicity (AOT) by gavage in a single dose of 2000 mg/Kg of body weight and Sub-Chronic Oral Toxicity (COT) tests by forced gavage of 200 mg of extract/D/Kg of body weight for 28 days were performed on female Wistar rats. The weight of the animals, serum creatinine, transaminases and the number of white blood cells were determined on day 0 and then on day 14 and day 28 respectively for AOT and COT. Histological analysis of liver, kidney and spleen was performed for both tests. Results: There were no deaths during toxicity testing and organ histology showed no atypia. Serum creatinine, serum levels of AST and ALT transaminases and the mean number of white blood cells did not vary significantly between the beginning and the end of the experiment for both AOT and COT. Only weight increased significantly (P < 0.05) for COT. Conclusion: The aqueous extract of the bark of Daniellia Oliveri did not show hepatic, renal and immune toxicity in acute or sub-chronic state. Its use in traditional medicine can be recommended.
