Table 1 - uploaded by Franco Biasioli
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List of considered clones.
Source publication
Proton Transfer Reaction-Mass Spectrometry
(PTR-MS) in its recently developed implementation based
on a time-of-flight mass spectrometer (PTR-ToF-MS) has
been evaluated as a possible tool for rapid non-destructive
investigation of the volatile compounds present in the
metabolome of apple cultivars and clones. Clone characterization
is a cutting-edg...
Context in source publication
Similar publications
Flavour profile characterization of apple cultivars is important to support fundamental research in apple physiology and genetics. This study applied Proton Transfer Reaction-Mass Spectrometry to monitor in-vitro and in-vivo flavour release of different apple cultivars. Both in-vivo and in-vitro results differentiated apple cultivars correlating fl...
Proton Transfer Reaction-Mass Spectrometry (PTR-MS) in its recently developed implementation based
on a time-of-flight mass spectrometer (PTR-ToF-MS) has been evaluated as a possible tool for rapid non-destructive investigation of the volatile compounds present in the metabolome of apple cultivars and clones. Clone characterization is a cutting-edg...
Citations
... [Place Figure 2 here] In the following cases, we report results obtained using the suggested tailored data analysis, for the three applications described in the protocol. We emphasize that the whole data analysis can be performed in one or a few days by tailored software developed in our laboratory 10,40 . In Figure 3, regarding VOC detection during lactic acid fermentation of yogurt (on-line bioprocess monitoring), we show different fermentation kinetics of nine selected mass peaks corresponding to four different commercial starter cultures 32 . ...
... Some rapid analysis, such as the nose-space, cannot be performed with GC based methods. Other studies screen of many samples using PTR-MS and GC as complementary approaches: PTR_MS allows the measurements of very large sample sets while GC analysis of reduced subsets provides additional information for a better interpretation of PTR-MS data 40 . On the other side, other rapid approaches have been proposed for VOC analysis, e.g., those based on e-noses or MS-e-noses or specific sensors. ...
Proton Transfer Reaction (PTR), combined with a Time-of-Flight (ToF) Mass Spectrometer (MS) is an analytical approach based on chemical ionization that belongs to the Direct-Injection Mass Spectrometric (DIMS) technologies. These techniques allow the rapid determination of volatile organic compounds (VOCs), assuring high sensitivity and accuracy. In general, PTR-MS requires neither sample preparation nor sample destruction, allowing real time and non-invasive analysis of samples. PTR-MS are exploited in many fields, from environmental and atmospheric chemistry to medical and biological sciences. More recently, we developed a methodology based on coupling PTR-ToF-MS with an automated sampler and tailored data analysis tools, to increase the degree of automation and, consequently, to enhance the potential of the technique. This approach allowed us to monitor bioprocesses (e.g. enzymatic oxidation, alcoholic fermentation), to screen large sample sets (e.g. different origins, entire germoplasms) and to analyze several experimental modes (e.g. different concentrations of a given ingredient, different intensities of a specific technological parameter) in terms of VOC content. Here, we report the experimental protocols exemplifying different possible applications of our methodology: i.e. the detection of VOCs released during lactic acid fermentation of yogurt (on-line bioprocess monitoring), the monitoring of VOCs associated with different apple cultivars (large-scale screening), and the in vivo study of retronasal VOC release during coffee drinking (nosespace analysis).
