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Bladder cancer (BC) is considered to be the tenth in the world. There is much evidence to suggest that the highly metastatic behaviour of BC may be due to the ability of its cells to undergo Epithelial Mesenchymal Transition (EMT).The S100 family of small Ca-binding proteins are among many genes that have been found to be implicated in BC cancer pr...
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... first-strand cDNA synthesis, primed with random primers, was performed using the protocol provided by the manufacturer ReverAid H minus First strand c DNA synthesis kit (Thermo Scientific, UK). Quantitative PCR was performed using set of primers provided by Sigma, UK and listed in Table 1. Real-time PCR reactions were performed in triplicate in a 20µl reaction volume containing 1· SYBRgreen mix (Applied Biosystems, USA), 600 nmol/l primers, and 1 ng cDNA. ...Similar publications
The alterations in migration, proliferation, and mechanics of cells observed during cancer progression can potentially be linked to enhanced tumor invasiveness. These properties are frequently attributed to the ability to form distant metastasis; however, the direct mutual connection between these properties is not always proven. Here, we studied t...
