Fig 2 - uploaded by Mohammad Zarei
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Left: Urinary tubes with calcium oxalate crystals in the ethylene glycol groups, magnified 10×40, H/E stained Right: Urinary tubes with calcium oxalate crystals in the ethylene glycol groups, magnified 10×40, H/E stained
Source publication
Aims: The objective of this research is to study the preventive effectiveness of Nigella sativa L. against rat kidney stones, generated by Ethylene Glycol. Methodology: In this study, 40 Wistar rats were categorized randomly into four groups of ten. During the research, drinking water labeled group A is mixed with mineral water through utilization...
Contexts in source publication
Context 1
... Control). However, in group B, Calcium Oxalate sediments were present in the renal tubes to the extent that in the microscopic analyses of the kidneys, considerable accumulation of Calcium Oxalate crystals was detected in the urinary tubes and other sections of the system such as Proximal, Distal, Loop of Henle, Collecting duct and Nephrons (Fig. 2, Negative Control). The number of Oxalate crystals was 28 in 10 microscopic fields, which acquires significant increase in comparison with group A ...
Citations
Urinary stones are a growing disease that results from pathological biomineralization. Cassia fistula Lin. is traditionally used to treat urinary stones. However, no scientific evidence is available to prove its antilithiatic effect. This study evaluates the antilithiatic potential of aqueous and ethanolic extract of Cassia fistula Lin. fruit (Cff) against calcium oxalate kidney stones. Forty‐two male Wistar rats were divided into seven groups ( n = 6/group): Group I (control), Group II (rats treated with ethylene glycol and ammonium chloride developed nephrolithiasis after 28 days), Group III (lithiatic rats receiving distilled water for 30 days), Group IV and V (lithiatic rats receiving aqueous extract of Cff at doses of 1 and 100 mg/kg body weight for 30 days, respectively) and Group VI and VII (lithiatic rats receiving ethanolic extract of Cff at doses of 1 and 100 mg/kg body weight for 30 days, respectively). Some parameters of urine and serum, and also renal oxidative stress and histopathology were used to determine the antilithiatic effect of aqueous and ethanolic extract of Cff. Therefore, the types of extracts of Cff improved abnormal levels of urine, serum, and renal oxidative stress and histopathology parameters. This antilithiatic effect of aqueous and ethanolic extracts of Cff, can be attributed to the anti‐crystallization and antioxidant properties of the extracts and the ability to improve urine and serum biochemistry.
Research Highlights
Ethylene glycol and ammonium chloride‐induced urolithiasis, aggregation of calcium oxalate deposits, increase of some urinary and serum parameters, relative kidney weight, kidney size and MDA activity, decrease of some urinary parameters, relative body weight and SOD activity.
Aqueous and ethanolic extracts of Cassia fistula Lin. lead to the treatment of urolithic rats by decreasing levels of urinary oxalate, phosphate, urea, serum urea, uric acid, creatinine, calcium, phosphate, MDA, kidney weight and kidney size, increasing levels of urinary calcium, creatinine, magnesium, citrate, body weight and SOD activity in the kidney, eliminating CaOx deposition (esp. ethanolic extract).
