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Lactophenol cotton blue mount conidial head radiate, vesicle sub-spherical to ellipsoidal, conidiogenous cells biseriate with phialides longer than or as long as metula.
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... Also, Aspergillus sydowii occurs in diverse soil and marine ecosystems [3]. Moreover, Aspergillus sydowii is a mesophilic soil saprobe that is a food contaminant as well as a human pathogen in immune-compromised patients [4]. Furthermore, the pathogenicity of Aspergillus sydowii depends on the genetic composition and immune status of the host [5]. ...
Aspergillus sydowii is a mesophilic soil saprobe that is a food contaminant as well as a human pathogen in immune-compromised patients. The biological fabrication of silica and silver nanoparticles provides advancements over the chemical approach, as it is eco-friendly and cost-effective. In the present study, Aspergillus sydowii isolates were collected from the soil fields of six different sites in the western area of Saudi Arabia and then identified using the PCR technique following sequencing analysis by BLAST and phylogenetic analysis. Then, applied silica and silver nanoparticles were synthesized by biological methods, using Aspergillus niger as a reducer. Silver and silica nanoparticles were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), and transmission electron microscopy (TEM). The antifungal activity of silver and silica nanoparticles against Aspergillus sydowii isolates was evaluated using the disc diffusion method and the minimum inhibitory concentration (MIC). The physiochemical results emphasized the fabrication of silver and silica nanoparticles in spherical shapes with a diameter in the range of 15 and 40 nm, respectively, without any aggregation. MIC of Ag-NPs and Si-NPs against Aspergillus sydowii isolates were 31.25 and 62.5 µg/mL, respectively. Finally, the aim of the study is the use of silver as well as silica nanoparticles as antifungal agents against Aspergillus sydowii.
... Soon after A. lentulus, one more cryptic species, Aspergillus thermomutans, was identified within the Fumigati section. Coral reefs are affected by an emerging fungal pathogen named Aspergillus sydowii, another cryptic species, also known as Emericella sydowii, which causes sinusitis, onychomycosis and keratomycosis [7]. The prevalence of cryptic Aspergillus species has been poorly investigated. ...
Introduction. Cryptic aspergillosis, caused by cryptic species of Aspergillus , is increasingly reported in humans and causes significant morbidity and mortality in immunocompromised individuals. The main aim of this study was to describe the occurrence of this entity at a large tertiary care centre and analyse the challenges in identifying them in a routine diagnostic laboratory.
Methods. This was a retrospective case review of all patients diagnosed with cryptic Aspergillus species from April 2019 to February 2020. The isolates were identified using conventional microbiological techniques, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI- TOF MS), 28S rRNA and internal transcribed spacer (ITS) sequencing.
Results. The species identified were Aspergillus tamarii, Aspergillus lentulus and Aspergillus sydowii . Identification by MALDI- TOF MS and sequencing was concordant for all except A. sydowii , with MALDI- TOF MS misidentifying it as Aspergillus thermomutans . All isolates showed low minimum inhibitory concentrations (MICs) for the panel of antifungal drugs.
Conclusion. Aspergillosis caused by cryptic Aspergillus species presents a diagnostic challenge. This study confirms the importance of molecular methods for accurate identification.
... 2 Heatmap of the nutrient assimilation by the isolated fungi Lee et al. 2016;Soler-Hurtado et al. 2016), so it is considered cosmopolitan. Both A. versicolor and A. sydowii have been isolated from humans and animals with respiratory conditions, although they are regarded as an unconventional pathogen (Zhang et al. 2012;Nagarajan et al. 2014). In the case of A. creber, according to Jurjević et al. (2012), it is a species very close phylogenetically to A. sydowii, and it is halotolerant. ...
In the aquaculture industry, the selection and quality of feed are highly relevant because their integrity and management have an impact on the health and development of organisms. In general, feeds contamination depends on storage conditions and formulation. Furthermore, it has been recognized that filamentous fungi are among the most important contaminating agent in formulated feeds. Therefore, the purpose of this research was to identify saprophytic fungi capable of proliferating in commercial feeds, as well as determining their prevalence, extracellular enzymes profile, ability to assimilate carbon sources, and finally their ability to produce aflatoxins. In order to do that, twenty-two fungi were isolated from commercial fish feeds. After, the species Aspergillus chevalieri, A. cristatus, A. sydowii, A. versicolor, A. flavus, A. creber, and Lichtheimia ramosa were identified. These fungi were able to produce extracellular enzymes, such as phosphatases, esterases, proteases, β-glucosidase, and N-acetyl-β-glucosaminidase. The isolated fungi showed no selective behavior in the assimilation of the different carbon sources, showing a strong metabolic diversity. Prevalence percentages above 85% were recorded. Among all fungi studied, A. flavus M3-C1 had the highest production of aflatoxins when this strain was inoculated directly in the feeds (295 ppb). The aflatoxin production by this strain under the experimental setting is above the permitted levels, and it has been established that high levels of aflatoxins in feeds can cause alterations in fish growth as well as the development of cancerous tumors in the liver, in addition to enhancing mortality.
... Several studies implicated that like other fungal infections, A. sydowii infection is difficult to manage. Itraconazole and voriconazole are two oral azole antifungal agents reported to have good activity against A. sydowii infection [36,47,49,50,54]. Our isolate showed low MIC for all the antifungal drugs tested. ...
Opportunistic fungal infections of the skin and nail are frequently encountered in human. Recent years have shown increased incidence of fungal infections especially in immunocompromised patients. Onychomycosis in HIV-infected patients is reported to occur in 15–40%, four times more than in the general population. Here, we report a case of fingernail proximal subungual onychomycosis with associated skin infection caused by an opportunistic mold, Aspergillus sydowii, in a HIV positive individual. Isolation of A. sydowii from nail and skin of an immunocompromised person needs accurate identification for successful treatment.
... It is apparently a terrestrial fungus, but it is salt tolerant and capable of growing in the sea [20]. Moreover, A. sydowii has been reported as a food contaminant [21], and a human pathogen in immune-compromised patients [22,23]. In marine ecosystems, A. sydowii has been isolated from some gorgonian communities of the Caribbean [11,24], Colombian Pacific coasts [25], and environmental samples of the Australian coastal waters [20]. ...
Fungal emerging pathogens are one of the main threats for global biodiversity. Sea turtles do not seem to be an exemption, and recent studies on important nesting areas worldwide have shown that two fungal pathogens, i.e. Fusarium falciforme and Fusarium keratoplasticum , are involved in low hatching success in nests of sea turtle species. Although the presence of these pathogens has been detected in Ascension Island, there are no investigations on the distribution of these two pathogens in main nesting beaches in the island. In this study, we analysed 109 eggshells of the species Chelonia mydas from four nesting areas in Ascension Island. We have isolated and identified a total of 46 fungal isolates. A phylogenetic analysis, of the ITS nrDNA region, with a number of reference sequences of the Fusarium solani species complex, showed that 23 of these isolates corresponded to the pathogen F. keratoplasticum . The analyses on isolation frequency, that included other previously obtained isolates, i.e. 11 F. keratoplasticum and one F. falciforme , showed that F. keratoplasticum was the species most frequently isolated in Ascension Island and it was found in all nesting beaches, while F. falciforme was only isolated from Pan Am beach. When compared with other nesting areas worldwide, the abundance of F. keratoplasticum over F. falciforme was higher than any other nesting region tested. These findings are important in order to evaluate the potential threat of this pathogen to nests of the sea turtle population of Ascension Island, and to develop future control strategies.
... It is apparently a terrestrial fungus, but it is salt tolerant and capable of growing in the sea [20]. Moreover, A. sydowii has been reported as a food contaminant [21], and a human pathogen in immune-compromised patients [22,23]. In marine ecosystems, A. sydowii has been isolated from some gorgonian communities of the Caribbean [11,24], Colombian Pacific coasts [25], and environmental samples of the Australian coastal waters [20]. ...
Emerging fungal diseases are threatening ecosystems and have increased in recent decades. In corals, the prevalence and consequences of these infections have also increased in frequency and severity. Coral reefs are affected by an emerging fungal disease named aspergillosis, caused by Aspergillus sydowii. This disease and its pathogen have been reported along the Caribbean and Pacific coasts of Colombia. Despite this, an impor- tant number of coral reefs worldwide have not been investigated for the presence of this pathogen. In this work, we carried out the surveillance of the main coral reef of the Ecuador- ian Pacific with a focus on the two most abundant and cosmopolitan species of this ecosys- tem, Leptogorgia sp. and Leptogorgia obscura. We collected 59 isolates and obtained the corresponding sequences of the Internal Transcribed Spacers (ITS) of the ribosomal DNA. These were phylogenetically analyzed using MrBayes, which indicated the presence of two isolates of the coral reef pathogen A. sydowii, as well as 16 additional species that are potentially pathogenic to corals. Although the analyzed gorgonian specimens appeared healthy, the presence of these pathogens, especially of A. sydowii, alert us to the potential risk to the health and future survival of the Pacific Ecuadorian coral ecosystem under the current scenario of increasing threats and stressors to coral reefs, such as habitat alter- ations by humans and global climate change.
... It is apparently a terrestrial fungus, but it is salt tolerant and capable of growing in the sea [20]. Moreover, A. sydowii has been reported as a food contaminant [21], and a human pathogen in immune-compromised patients [22,23]. In marine ecosystems, A. sydowii has been isolated from some gorgonian communities of the Caribbean [11,24], Colombian Pacific coasts [25], and environmental samples of the Australian coastal waters [20]. ...
Emerging fungal diseases are threatening ecosystems and have increased in recent decades. In corals, the prevalence and consequences of these infections have also increased in frequency and severity. Coral reefs are affected by an emerging fungal disease named aspergillosis, caused by Aspergillus sydowii. This disease and its pathogen have been reported along the Caribbean and Pacific coasts of Colombia. Despite this, an important number of coral reefs worldwide have not been investigated for the presence of this pathogen. In this work, we carried out the surveillance of the main coral reef of the Ecuador-ian Pacific with a focus on the two most abundant and cosmopolitan species of this ecosystem , Leptogorgia sp. and Leptogorgia obscura. We collected 59 isolates and obtained the corresponding sequences of the Internal Transcribed Spacers (ITS) of the ribosomal DNA. These were phylogenetically analyzed using MrBayes, which indicated the presence of two isolates of the coral reef pathogen A. sydowii, as well as 16 additional species that are potentially pathogenic to corals. Although the analyzed gorgonian specimens appeared healthy, the presence of these pathogens, especially of A. sydowii, alert us to the potential risk to the health and future survival of the Pacific Ecuadorian coral ecosystem under the current scenario of increasing threats and stressors to coral reefs, such as habitat alterations by humans and global climate change.
Aspergillus sydowii is a cosmopolitan fungus that has been responsible for the mass destruction of coral in the Caribbean Sea over the last 15 years. To our knowledge, this study has found the first case of A. sydowii in the Mediterranean Sea, in marine-bottom sediments, water and calcareous shells of bivalve molluscs sampled during a campaign to characterise the mycobiota in the Port of Genoa (Italy). The area is characterised by adverse environmental conditions (high turbidity, organic pollution and high concentrations of phosphorus and nitrogen compounds). These parameters, in combination with a rising temperature, could contribute to A. sydowii bloom and dispersal. This fungal strain may have been imported into the Port of Genoa in the bilge water of vessels or by torrent input. This work represents the first step in the implementation of a monitoring programme to safeguard calcareous sponges and sea fan corals endemic in the Mediterranean Sea.
