Karyotype and phenotype in hexaploid and nonaploid wheat. (A) and (B), FISH and GISH images of a metaphase cell of hexaploid wheat. (C) and (D), FISH and GISH images of a metaphase cell of nonaploid wheat. (E) Seedlings of hexaploid and nonaploid wheat. The FISH probes used are pSc119.2 (green), and pAS1 (red), the GISH probes used are genomic DNA from Triticum urartu (genome AA, green), and Aegilops tauchii (genome DD, red); and genome BB (blue) was counterstained with DAPI.