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Isolation and purification of algae. a) Algae stock cultures, b) Streaking purification of the microalgae on semi-solid BG-11 agar plates, c) Axenic culture preserved in cell culture flasks.

Isolation and purification of algae. a) Algae stock cultures, b) Streaking purification of the microalgae on semi-solid BG-11 agar plates, c) Axenic culture preserved in cell culture flasks.

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Selection of appropriate strains of microalgae that work well in local conditions is important for a establish algae-based production system. The general aim of this study was to isolate and identify native microalgae species for exploring its further potential applications. To achieve this aim, 25 samples were collected from different locations of...

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... each diluted tube was cultured in both liquid and semi-solid agar plates media, incubated in controlled conditions at 22 ± 1°C, exposed to continues light using LeD fluorescent tube of intensity 2000 LuX 28 μmol.m -2 . s -1 ( figure 1a). further purification was achieved by consecutive streaking on semi-solid bG-11 agar (figure 1b). ...