Intrinsic fluorescence spectra (A); Fluorescent intensity and maximum position of fluorescence peak (λ max ) (B); UV second derivative spectra (C); Zetapotential (ζ) and effective particle diameter (d eff ) of myofibrillar protein from red sea bream (D) treated by different thawing methods. (Different lowercase and uppercase letters indicate significant differences between the means of fluorescent intensity and λ max , respectively in Fig. 5B (p < 0.05); Different lowercase and uppercase letters indicate significant differences between the means of d eff and ζ, respectively in Fig. 5D (p < 0.05)).

Contexts in source publication

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... stretching vibration intensity of phenylalanine at 1003 cm 1 was normalized as the internal standard, and the Raman spectrum of MFP was obtained from Fig. 4A, of which the amide I band (1600 ~ 1700 cm 1 ) underwent deconvolution and curve fitting, as shown in Fig. S5. Then, the secondary structure of MFP, The Raman spectrum at 500 ~ 550 cm 1 is the characteristic stretching vibration band of disulfide bridges (S-S). The conformations of S -S at 515-525 cm 1 and 535-545 cm 1 correspond to gauchegauche-trans (g-g-t) and trans-gauche-trans (t-g-t), respectively, among which the t-g-t conformation is ...

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... the intrinsic fluorescence spectrum can be used to reflect changes in the micro-environment of Trp residues, which is an important technical method to detect protein tertiary conformations. From Fig. 5A and 5B, the MFP of CON showed the highest fluorescence intensity (FI) (795.3) at the maximum position of the fluorescence peak (λ max ) (332.3 nm). Compared with CON, the λ max of RT, HVEF, US, and US&HVEF showed a red shift (to longer wavelengths) of 2.1, 1.3, 1.0, and 0.5 nm, respectively. The FI decreased to 339.84, 433.67, 477.23, ...

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... distance between the peak and trough of the first and second absorption peaks in the UV second derivative absorption spectrum was recorded as "a" and "b", respectively (Fig. 5C). The r value (r = a/b) represents the change in polarity of the Tyr residue microenvironment and the protein structural unfolding (Cai et al., 2018). From Fig. 5C, the r values after HVEF, US, and US&HVEF treatment were significantly increased by 0.92 %, 9.17 %, and 3.67 %, respectively, compared with the RT sample ...

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... r value (r = a/b) represents the change in polarity of the Tyr residue microenvironment and the protein structural unfolding (Cai et al., 2018). From Fig. 5C, the r values after HVEF, US, and US&HVEF treatment were significantly increased by 0.92 %, 9.17 %, and 3.67 %, respectively, compared with the RT sample (1.09). ...

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... the aggregation degree of MFP was characterized by zetapotential and particle size, as displayed in Fig. 5D and Fig. S6 (Fig. 5D). The average effective particle diameter (d eff ) was 2641.84 nm, 1920.89 nm, 1621.94 nm, and 1340.06 nm, respectively, as compared with CON (1037.18 nm) ( Fig. 5D and Fig. ...

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... the aggregation degree of MFP was characterized by zetapotential and particle size, as displayed in Fig. 5D and Fig. S6 (Fig. 5D). The average effective particle diameter (d eff ) was 2641.84 nm, 1920.89 nm, 1621.94 nm, and 1340.06 nm, respectively, as compared with CON (1037.18 nm) ( Fig. 5D and Fig. ...

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... the aggregation degree of MFP was characterized by zetapotential and particle size, as displayed in Fig. 5D and Fig. S6 (Fig. 5D). The average effective particle diameter (d eff ) was 2641.84 nm, 1920.89 nm, 1621.94 nm, and 1340.06 nm, respectively, as compared with CON (1037.18 nm) ( Fig. 5D and Fig. ...

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... addition, the conformational changes of MFP can be effectively monitored by measuring the intrinsic fluorescence spectrum of Trp residues (Fig. 5B). In addition, UV second derivative absorption spectra were used to analyze the changes in the microenvironment of aromatic amino acid residues, such as tyrosine (Tyr) and tryptophan (Trp) (Fig. 5C). The results indicated that US&HVEF technology has a synergistic bacteriostatic effect, the degree of protein oxidation during thawing was ...

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... addition, the conformational changes of MFP can be effectively monitored by measuring the intrinsic fluorescence spectrum of Trp residues (Fig. 5B). In addition, UV second derivative absorption spectra were used to analyze the changes in the microenvironment of aromatic amino acid residues, such as tyrosine (Tyr) and tryptophan (Trp) (Fig. 5C). The results indicated that US&HVEF technology has a synergistic bacteriostatic effect, the degree of protein oxidation during thawing was reduced, which effectively maintained the stability of the protein spatial ...

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... the ζ value ranged from 16 to 30 mV, which meant the threshold of delicate dispersion of protein solution, and from 30 to 40 mV indicated the moderate stability of solution ( Beliciu & Moraru, 2011). From Fig. 5D, after fillets were thawed, the |ζ| value of US&HVEF samples was the highest, and the d eff was the lowest, indicating that the electrostatic repulsion between protein molecules was enhanced, and the degree of aggregation in protein molecules was weakened, resulting in a smaller protein particle size and the enhanced solution stability. The linear relationship between antibacterial efficiency and the degree of protein aggregation was investigated. ...