FIG 2 - uploaded by Clement K.M. Tsui
Content may be subject to copyright.
Interference contrast micrographs of taxa from Helicosporium and Helicomyces. a–c. Conidiophores, conidiogenous cells and conidia of Helicosporium vegetum. d. Conidiophores with tooth-like conidiogenous cells of Helicosporium pallidum. e. Conidiophore of Helicosporium guianensis bearing bladder-like conidiogenous cells. f. Conidia of Helicosporium pallidum. g. Conidiophores of Helicomyces roseus. h. Conidia of Helicomyces lilliputeus. Bars: a 5 30 mm, b 5 7 mm, c, d, g 5 20 mm, e 5 40 mm, f 5 10 mm, h 5 12 mm.
Source publication
Three genera of asexual, helical-spored fungi, Helicoma, Helicomyces and Helicosporium traditionally have been differentiated by the morphology of their conidia and conidiophores. In this paper we assessed their phylogenetic relationships from ribosomal sequences from ITS, 5.8S and partial LSU regions using maximum parsimony, maximum likelihood and...
Citations
... Based on the two-gene tree, we speculated that Isthmolongispora is polyphyletic. So far, at least 14 genera of aquatic hyphomycetes have shown to be polyphyletic using sequence information from a single or two genes (Nikolcheva 2002;Tsui et al. 2006;Baschien 2006;Campbell et al. 2006;Duarte et al. 2015). ...
The family Microthyriaceae is represented by relatively few mycelial cultures and DNA sequences; as a result, the taxonomy and classification of this group of organisms remain poorly understood. During the investigation of the diversity of aquatic hyphomycetes from southern China, several isolates were collected. These isolates were cultured and sequenced and a BLAST search of its LSU sequences against data in GenBank revealed that the closest related taxa are in the genus Microthyrium . Phylogenetic analyses, based on the combined sequence data from the internal transcribed spacers (ITS) and the large subunit (LSU), revealed that these isolates represent eight new taxa in Microthyriaceae, including two new genera, Antidactylaria gen. nov. and Isthmomyces gen. nov. and six new species, Antidactylaria minifimbriata sp. nov. , Isthmomyces oxysporus sp. nov. , I. dissimilis sp. nov. , I. macrosporus sp. nov. , Triscelophorus anisopterioideus sp. nov. and T. sinensis sp. nov . These new taxa are described, illustrated for their morphologies and compared with similar taxa. In addition, two new combinations are proposed in this family.
... Dictyosporium-like species have distinctive sporodochial conidiomata and septate, brown to dark brown, cheirosporous, finger-or palm-like conidia (like a hand with the fingers binding together). Initial phylogenetic analyses of ITS and LSU rDNA sequence data (Tsui et al. 2006) suggest that Dictyosporium sensu lato was paraphyletic. Tanaka et al. (2015) revised several families in the suborder Massarineae including Dictyosporiaceae based on a combination of LSU, SSU, and TEF1α sequence data and showed that Dictyosporiaceae forms a robust clade amongst other families in a suborder Massarineae. ...
This study introduces, Paradictyocheirospora, a new genus, belonging to the family Dictyosporiaceae with evidence from morphology and phylogenetic analysis of a combined ITS, LSU and TEF1α sequence data. Paradictyocheirospora can be distinguished from allied taxa based on dictyosporous, cheiroid conidia with suprabasal bubble-like hyaline appendages and cylindrical, reduced conidiophores. The new genus is similar to Dictyocheirospora; however, the conidia are smaller with fewer rows (3–6) and have globose, supra-basal appendages. Paradictyocheirospora tectonae forms a distinct lineage basal to Digitodesmium and Dictyocheirospora. Additional new collections from Thane, Maharashtra were morphologically identical and phylogenetically related to Dictyocheirospora nabanheensis, and the latter is a new record from India.
... The most obvious result achieved on the phylogeny of aquatic hyphomycetes is that the multiple origins of aquatic hyphomycetes was found [22]. So far, at least 14 genera have shown to be polyphyletic using sequence information from a single or two genes [23][24][25][26]78]. Likewise, polyphyly of Isthmolongispora was found for the first time here. ...
The fungal family Microthyriaceae is represented by relatively few mycelial cultures and DNA sequences. As a result, the taxonomy and classification of this group of organisms remain poorly understood. Here, based on DNA sequences at four gene fragments (nuLSU rDNA, nuSSU rDNA, TEF1 and RPB2) in our analyses of aquatic hyphomycetes from southern China, we identify and report four new genera (Antidactylaria, Isthmomyces, Keqinzhangia, Pseudocoronospora) and thirteen new species (Antidactylaria minifimbriata, Pseudocoronospora hainanensis, Isthmomyces oxysporus, I. dissimilis, I. macrosporus, I. relanceatus, Keqinzhangia aquatica, Triscelophorus anakonajensis, T. anisopterioides, T. guizhouensis, T. mugecuoensis, T. multibrachiatus, T. neoseptatus; new combinations Isthmomyces asymmetrica, I. basitruncata, I. geniculata, I. lanceata, I. minima, I. rotundata) belonging to Microthyriaceae. Our results provided the first molecular evidence of asexual morph of this family and strengthened the phylogenetic placement of the family in class Dothideomycetes. The addition of these new taxa made Microthyriaceae the largest family comprising freshwater asexual genera in Pleosporomycetidae. In addition, we confirmed the monophyly of the genus Triscelophorus, the paraphyly of the genus Isthmolongispora, and revised 6 new combinations in Isthmolongispora. ITS barcoding of 13 species were also provided to help identify aquatic hyphomycetes in the future. Our results suggest that the asexual genera and sexual genera identified so far within this family have completely different ecological niches.
... On MEA, PDA and OA surface brown vinaceous, reverse honey. Notes -Neohelicomyces differs from Tubeufia and Helicomyces in having elongate, erect, conspicuous conidiophores (Tsui et al. 2006, Crous et al. 2019b. Neohelicomyces melaleucae is closely related to 'Tubeufia' helicomyces (CBS 272.52) and N. pandanicola but is distinct based on its DNA phylogeny. ...
Novel species of fungi described in this study include those from various countries as follows: Antarctica , Apenidiella antarctica from permafrost, Cladosporium fildesense from an unidentified marine sponge. Argentina , Geastrum wrightii on humus in mixed forest. Australia , Golovinomyces glandulariae on Glandularia aristigera , Neoanungitea eucalyptorum on leaves of Eucalyptus grandis , Teratosphaeria corymbiicola on leaves of Corymbia ficifolia , Xylaria eucalypti on leaves of Eucalyptus radiata . Brazil , Bovista psammophila on soil, Fusarium awaxy on rotten stalks of Zea mays , Geastrum lanuginosum on leaf litter covered soil, Hermetothecium mikaniae-micranthae (incl. Hermetothecium gen. nov.) on Mikania micrantha , Penicillium reconvexovelosoi in soil, Stagonosporopsis vannaccii from pod of Glycine max . British Virgin Isles , Lactifluus guanensis on soil. Canada , Sorocybe oblongispora on resin of Picea rubens . Chile , Colletotrichum roseum on leaves of Lapageria rosea . China , Setophoma caverna from carbonatite in Karst cave. Colombia , Lareunionomyces eucalypticola on leaves of Eucalyptus grandis . Costa Rica , Psathyrella pivae on wood. Cyprus , Clavulina iris on calcareous substrate. France , Chromosera ambigua and Clavulina iris var. occidentalis on soil. French West Indies , Helminthosphaeria hispidissima on dead wood. Guatemala , Talaromyces guatemalensis in soil. Malaysia , Neotracylla pini (incl. Tracyllales ord. nov. and Neotracylla gen. nov.) and Vermiculariopsiella pini on needles of Pinus tecunumanii . New Zealand , Neoconiothyrium viticola on stems of Vitis vinifera , Parafenestella pittospori on Pittosporum tenuifolium , Pilidium novae-zelandiae on Phoenix sp. Pakistan , Russula quercus-floribundae on forest floor. Portugal , Trichoderma aestuarinum from saline water. Russia , Pluteus liliputianus on fallen branch of deciduous tree, Pluteus spurius on decaying deciduous wood or soil. South Africa , Alloconiothyrium encephalarti , Phyllosticta encephalarticola and Neothyrostroma encephalarti (incl. Neothyrostroma gen. nov.) on leaves of Encephalartos sp., Chalara eucalypticola on leaf spots of Eucalyptus grandis × urophylla , Clypeosphaeria oleae on leaves of Olea capensis , Cylindrocladiella postalofficium on leaf litter of Sideroxylon inerme , Cylindromonium eugeniicola (incl. Cylindromonium gen. nov.) on leaf litter of Eugenia capensis , Cyphellophora goniomatis on leaves of Gonioma kamassi , Nothodactylaria nephrolepidis (incl. Nothodactylaria gen. nov. and Nothodactylariaceae fam. nov.) on leaves of Nephrolepis exaltata , Falcocladium eucalypti and Gyrothrix eucalypti on leaves of Eucalyptus sp., Gyrothrix oleae on leaves of Olea capensis subsp. macrocarpa , Harzia metrosideri on leaf litter of Metrosideros sp., Hippopotamyces phragmitis (incl. Hippopotamyces gen. nov.) on leaves of Phragmites australis , Lectera philenopterae on Philenoptera violacea , Leptosillia mayteni on leaves of Maytenus heterophylla , Lithohypha aloicola and Neoplatysporoides aloes on leaves of Aloe sp., Millesimomyces rhoicissi (incl. Millesimomyces gen. nov.) on leaves of Rhoicissus digitata , Neodevriesia strelitziicola on leaf litter of Strelitzia nicolai , Neokirramyces syzygii (incl. Neokirramyces gen. nov.) on leaf spots of Syzygium sp., Nothoramichloridium perseae (incl. Nothoramichloridium gen. nov. and Anungitiomycetaceae fam. nov.) on leaves of Persea americana , Paramycosphaerella watsoniae on leaf spots of Watsonia sp., Penicillium cuddlyae from dog food, Podocarpomyces knysnanus (incl. Podocarpomyces gen. nov.) on leaves of Podocarpus falcatus , Pseudocercospora heteropyxidicola on leaf spots of Heteropyxis natalensis , Pseudopenidiella podocarpi , Scolecobasidium podocarpi and Ceramothyrium podocarpicola on leaves of Podocarpus latifolius , Scolecobasidium blechni on leaves of Blechnum capense , Stomiopeltis syzygii on leaves of Syzygium chordatum , Strelitziomyces knysnanus (incl. Strelitziomyces gen. nov.) on leaves of Strelitzia alba , Talaromyces clemensii from rotting wood in goldmine, Verrucocladosporium visseri on Carpobrotus edulis . Spain , Boletopsis mediterraneensis on soil, Calycina cortegadensisi on a living twig of Castanea sativa , Emmonsiellopsis tuberculata in fluvial sediments, Mollisia cortegadensis on dead attached twig of Quercus robur , Psathyrella ovispora on soil, Pseudobeltrania lauri on leaf litter of Laurus azorica , Terfezia dunensis in soil, Tuber lucentum in soil, Venturia submersa on submerged plant debris. Thailand , Cordyceps jakajanicola on cicada nymph, Cordyceps kuiburiensis on spider, Distoseptispora caricis on leaves of Carex sp., Ophiocordyceps khonkaenensis on cicada nymph. USA , Cytosporella juncicola and Davidiellomyces juncicola on culms of Juncus effusus , Monochaetia massachusettsianum from air sample, Neohelicomyces melaleucae and Periconia neobrittanica on leaves of Melaleuca styphelioides × lanceolata , Pseudocamarosporium eucalypti on leaves of Eucalyptus sp., Pseudogymnoascus lindneri from sediment in a mine, Pseudogymnoascus turneri from sediment in a railroad tunnel, Pulchroboletus sclerotiorum on soil, Zygosporium pseudomasonii on leaf of Serenoa repens . Vietnam , Boletus candidissimus and Veloporphyrellus vulpinus on soil. Morphological and culture characteristics are supported by DNA barcodes.
... The finding of only two genera of entomopathogenic fungi in the samples coincides with what was reported by other authors, who report that although entomopathogenic fungi are common inhabitants of the soil, their diversity is low, with only one or two species coexisting (Goble et al. 2010;Quesada-Moraga et al. 2007;Keller et al. 2003). Nonetheless, as in many genera of the Hypocreales order, the Metarhizium species could not be easily distinguished based on morphological features (Rehner and Buckley 2005;Tsui et al. 2006). Based on the data for EF-1a, all the 12 Metarhizium strains isolated from the wine-growing area in Argentina were identified as Metarhizium robertsii. ...
Biological control by entomopathogenic fungi is a possible alternative to chemical insecticides. As the grapevine moth Lobesia botrana (Lepidoptera: Tortricidae), recently introduced in Argentina, is now the major pest in most of the vineyards in the country despite quarantine regulations, native entomopathogenic fungi could be a preferable alternative to current synthetic insecticides. Thus, the aim of this study was to characterize native fungus strains isolated from 45 soil samples, using larvae of L. botrana as bait insect, and infected arthropods from the wine-growing region in the west of Argentina. Sixteen strains were identified, belonging to two species: four strains to Beauveria bassiana (Hypocreales: Cordycipitaceae) and 12 strains to Metarhizium robertsii (Hypocreales: Clavicipitaceae). Based on their physiological features, M. robertsii strains collected from the west region of Argentina seem to be the most tolerant to the high temperatures specific to this region. Therefore, being well adapted to Argentinean climate conditions, some of the native M. robertsii could be proposed as biological control agents against L. botrana.
... Further parameters are conditions of seaweed such as age [19,34]. Moreover, the chemical forms of minerals and their background levels in seawater may influence the absorption of minerals by seaweed [35]. Therefore, the accumulation of certain minerals by seaweed can be greater than the concentration of the same elements in the surroundings by many orders of magnitude [36]. ...
The Red Sea is largely undiscovered, strange wellspring of bioactive materials that its waters have not received sufficient and broad inspection till date. It expands for approximately 2000 kms and its semi segregation jointly with a rising saltiness at high water temperatures have offered new research trends for biological communities and developmental adjustments. Just a couple of marine green growths have been accounted for from the Red Sea up until now (27 passages in Algae Base rather than 512 for the Caribbean and 307 for the Arabian Gulf) and researches exploring the bioactivity of Red Sea algae are not many. Marine living beings have ended up being a rich wellspring of unprecedented and hopeful bioactive atoms for an extensive variety of uses, including novel therapeutics, cosmetics, and biotechnological applications. Marine algae, beside whichever benthic organisms, are predominately influenced by marine biofouling (epibiosis). It was confirmed that the aforementioned creatures yield secondary metabolites with antialgal, antibacterial, antifungal, anti-macrofouling and antiprotozoal characteristics in order to retain their surfaces without epibionts. Accordingly, natural yields from marine algae prove to be a favourable alternate source of unprecedented ecologically friendly compounds. Interestingly, exposing algae to light and high oxygen concentrations will stimulate the formation of inflammatory mediators like ROS and NOS. Consequently, algae are capable to produce the substantial compounds in order to protect themselves from external factors like UV radiation, stress and pollution. Generally, natural products are the main origin of compounds utilized in cancer therapy with over 75% of antineoplastic drugs in clinical research trials being either acquired or at least created by nature. Hence, marine algae possess a particular function since they are to an increasing extent significant dietary constituent in considerable portions of the world and are discussed as prospective, pharmaceutical foods in cancer management. In addition to the anti-cancer activity, marine algae display a multitude of anti-viral activities with an essential number of investigations concentrating on the human immunodeficiency virus type 1 (HIV-1), thereby exploring the significance of the aforementioned viral pathogen. HIV-1 keeps being a considerable human being health concern since there are more than 35.3 million people infected globally and 2.3 million new infections annually. Algal compounds were found to attack different steps of HIV-1 replication, covering viral entry and the main viral enzymes such as Reverse Transcriptase (RT), Integrase, and Protease. Furthermore, epidemiological discoveries indicate a consumption connection of marine algae with a low prevalence of HIV/AIDS in several Eastern Asia locations.
... Identities = 321/402 (80 %), 30 gaps (7 %)). Notes: Neohelicomyces differs from Tubeufia and allied genera, especially from Helicomyces, in having elongate, erect, conspicuous conidiophores, and differs from Helicosporium based on conidial morphology (Tsui et al. 2006). Based on the species known from their DNA, N. deschampsiae appears to represent a new species, being phylogenetically distinct from T. helicomyces and T. paludosa, which have also been reported from this host (Ellis & Ellis 1997). ...
One order, seven families, 28 new genera, 72 new species, 13 new combinations, four epitypes, and interesting new host and / or geographical records are introduced in this study. Pseudorobillardaceae is introduced for Pseudorobillarda (based on P. phragmitis). New genera include: Jeremyomyces (based on J. labinae) on twigs of Salix alba (Germany); Neodothidotthia (based on N. negundinicola) on Acer negundo (Ukraine); Neomedicopsis (based on N. prunicola) on fallen twigs of Prunus padus (Ukraine); Neophaeoappendicospora (based on N. leucaenae) on Leucaena leucocephala (France) (incl. Phaeoappendicosporaceae); Paradevriesia (incl. Paradevriesiaceae) (based on P. americana) from air (USA); Phaeoseptoriella (based on P. zeae) on leaves of Zea mays (South Africa); Piniphoma (based on P. wesendahlina) on wood debris of Pinus sylvestris (Germany); Pseudoconiothyrium (based on P. broussonetiae) on branch of Broussonetia papyrifera (Italy); Sodiomyces (based on S. alkalinus) from soil (Mongolia), and Turquoiseomyces (incl. Turquoiseomycetales and Turquoiseomycetaceae) (based on T. eucalypti) on leaves of Eucalyptus leptophylla (Australia); Typhicola (based on T. typharum) on leaves of Typha sp. (Germany); Xenodevriesia (incl. Xenodevriesiaceae) (based on X. strelitziicola) on leaves of Strelitzia sp. (South Africa). New species include: Bacillicladium clematidis on branch of Clematis vitalbae (Austria); Cercospora gomphrenigena on leaves of Gomphrena globosa (South Africa); Cyphellophora clematidis on Clematis vitalba (Austria); Exophiala abietophila on bark of Abies alba (Norway); Exophiala lignicola on fallen decorticated trunk of Quercus sp. (Ukraine); Fuscostagonospora banksiae on Banksia sp. (Australia); Gaeumannomycella caricicola on dead leaf of Carex remota (Germany); Hansfordia pruni on Prunus persica twig (Italy) (incl. Hansfordiaceae); Microdochium rhopalostylidis on Rhopalostylis sapida (New Zealand); Neocordana malayensis on leaves of Musa sp. (Malaysia); Neocucurbitaria prunicola on fallen twigs of Prunus padus (Ukraine); Neocucurbitaria salicis-albae on Salix alba twig (Ukraine); Neohelicomyces deschampsiae on culm base of dead leaf sheath of Deschampsia cespitosa (Germany); Pararoussoella juglandicola on twig of Juglans regia (Germany); Pezicula eucalyptigena on leaves of Eucalyptus sp. (South Africa); Phlogicylindrium dunnii on leaves of Eucalyptus dunnii (Australia); Phyllosticta hagahagaensis on leaf litter of Carissa bispinosa (South Africa); Phyllosticta austroafricana on leaf spots of unidentified deciduous tree host (South Africa); Pseudosigmoidea alnicola on Alnus glutinosa leaf litter (Germany); Pseudoteratosphaeria africana on leaf spot on unidentified host (Angola); Porodiplodia vitis on canes of Vitis vinifera (USA); Sodiomyces alkalinus from soil (Mongolia), Sodiomyces magadiensis and Sodiomyces tronii from soil (Kenya), Sympodiella quercina on fallen leaf of Quercus robur (Germany) and Zasmidium hakeicola on leaves of Hakea corymbosa (Australia). Epitypes are designated for: Cryptostictis falcata on leaves of E. alligatrix (Australia), Hendersonia phormii on leaves of Phormium tenax (New Zealand), Sympodiella acicola on needles of Pinus sylvestris (Netherlands), and Sphaeria scirpicola var. typharum on leaf of Typha sp. (Germany). Several taxa originally described from rocks are validated in this study. New taxa include: Extremaceae fam. nov., and new genera, Arthrocatena, Catenulomyces, Constantinomyces, Extremus, Hyphoconis, Incertomyces, Lapidomyces, Lithophila,Monticola, Meristemomyces, Oleoguttula, Perusta, Petrophila, Ramimonilia, Saxophila and Vermiconidia. New species include: Arthrocatena tenebrosa, Catenulomyces convolutus, Constantinomyces virgultus, C. macerans, C. minimus,C. nebulosus, C. virgultus, Exophiala bonariae, Extremus adstrictus, E. antarcticus, Hyphoconis sterilis, Incertomyces perditus, Knufia karalitana, K. marmoricola, K. mediterranea, Lapidomyces hispanicus, Lithophila guttulata, Monticola elongata, Meristemomyces frigidus, M. arctostaphyli, Neodevriesia bulbillosa, N. modesta, N. sardiniae, N. simplex, Oleoguttula mirabilis, Paradevriesia compacta, Perusta inaequalis, Petrophila incerta, Rachicladosporium alpinum, R. inconspicuum, R. mcmurdoi, R. monterosanum, R. paucitum, Ramimonilia apicalis, Saxophila tyrrhenica, Vermiconidia antarctica, V. calcicola, V. foris, and V. flagrans.
... Subsequently, molecular taxonomic studies of the fungal genus Metarhizium Sorokin (Hypocreales; Clavicipitaceae) have increased considerably in recent years. However, species delineation within this genus, as in many other entomopathogenic fungal genera, has remained a difficult task on the basis of morphological characteristics and ITS sequence data alone (Crous et al., 2005;Rehner and Buckley, 2005;Tsui et al., 2006). A more sensitive and robust basis for molecular taxonomic studies of Metarhizium fungi was achieved by the introduction (Bischoff et al., 2006(Bischoff et al., , 2009 and successful application (Kepler et al., 2014;Montalva et al., 2016;Rehner and Kepler, 2017) of an additional Multilocus Sequence Analysis (MLSA) scheme. ...
The aim of this study was to search for entomopathogenic fungi that infect wild cockroaches in forest ecosystems in two protected natural areas of Argentina. Two isolates of Metarhizium argentinense were obtained and identified from wild cockroaches (Blaberidae: Epilamprinae) through the use of morphological characteristics and molecular phylogenetic analyses. This novel species was found in Argentina and is a member of the Metarhizium flavoviride species complex. Phylogenetic analyses, based on sequence similarity analysis using internal transcribed spacer (ITS) and a set of four protein-coding marker sequences (EF1A, RPB1, RPB2 and BTUB), supported the status of this fungus as a new species. In addition, we tested the biological activity of the new species through assays against Blattella germanica nymphs and found that the two evaluated isolates were pathogenic. However, isolate CEP424 was more virulent and caused a confirmed mortality of 76 % with a median lethal time of 7.2 d. This study reports the southernmost worldwide location of a Metarhizium species that infects cockroaches and will help expand the knowledge of the biodiversity of pathogenic fungi of Argentine cockroaches.
... j Germinating conidium. Scale bars: a = 200 lm, b = 50 lm, c-f = 10 lm, g-j = 5 lm Fungal Diversity with hyaline ascospores (sexual morph) and producing helicosporous conidia (asexual morph) (Tsui et al. 2006;Boonmee et al. 2011Boonmee et al. , 2014. There are 25 genera in the family (Wijayawardene et al. 2018). ...
This paper provides illustrated descriptions of micro-fungi newly found on Pandanaceae in China and Thailand. The fungi are accommodated in 31 families. New taxa described include a new family, seven new genera, 65 new species, 16 previously known species. A new family: Malaysiascaceae (Glomerellales). New genera are Acremoniisimulans (Plectosphaerellaceae), Pandanaceomyces, Pseudoachroiostachy (Nectriaceae), Pseudohyaloseta (Niessliaceae), Pseudoornatispora (Stachybotriaceae) and Yunnanomyces (Sympoventuriaceae). New species are Acremoniisimulans thailandensis, Beltrania krabiensis, Beltraniella pandanicola, B. thailandicus, Canalisporium krabiense, C. thailandensis, Clonostachys krabiensis, Curvularia chonburiensis, C. pandanicola, C. thailandicum, C. xishuangbannaensis, Cylindrocladiella xishuangbannaensis, Dictyochaeta pandanicola, Dictyocheirospora nabanheensis, D. pandanicola, D. xishuangbannaensis, Dictyosporium appendiculatum, Di. guttulatum, Di. hongkongensis, Di. krabiense, Di. pandanicola, Distoseptispora thailandica, D. xishuangbannaensis, Helicoma freycinetiae, Hermatomyces biconisporus, Lasiodiplodia chonburiensis, L. pandanicola, Lasionectria krabiense, Menisporopsis pandanicola, Montagnula krabiensis, Musicillium pandanicola, Neofusicoccum pandanicola, Neohelicomyces pandanicola, Neooccultibambusa thailandensis, Neopestalotiopsis chiangmaiensis, N. pandanicola, N. phangngaensis, Pandanaceomyces krabiensis, Paracylindrocarpon nabanheensis, P. pandanicola, P. xishuangbannaensis, Parasarcopodium hongkongensis, Pestalotiopsis krabiensis, P. pandanicola, Polyplosphaeria nabanheensis, P. pandanicola, P. xishuangbannaensis, Pseudoachroiostachys krabiense, Pseudoberkleasmium pandanicola, Pseudochaetosphaeronema pandanicola, Pseudohyaloseta pandanicola, Pseudoornatispora krabiense, Pseudopithomyces pandanicola, Rostriconidium pandanicola, Sirastachys phangngaensis, Stictis pandanicola, Terriera pandanicola, Thozetella pandanicola, Tubeufia freycinetiae, T. parvispora, T. pandanicola, Vermiculariopsiella hongkongensis, Volutella krabiense, V. thailandensis and Yunnanomyces pandanicola. Previous studies of micro-fungi on Pandanaceae have not included phylogenetic support. Inspiration for this study came from the book Fungi Associated with Pandanaceae by Whitton, McKenzie and Hyde in 2012. Both studies reveal that the micro-fungi on Pandanaceae is particularly rich in hyphomycetes. All data presented herein are based on morphological examination of specimens, coupled with phylogenetic sequence data to better integrate taxa into appropriate taxonomic ranks and infer their evolutionary relationships.
... Several studies have demonstrated that these mitosporic hyphomycetes are of terrestrial origin; yet, understanding of the relationships between the ecological roles of mitosporic fungi and their sexual states remains a major challenge in fungal ecology. Molecular phylogenetic techniques are currently being used to integrate mitotic morphs into the phylogeny of the meiotic ascomycetes (Belliveau and Bärlocher, 2005;Baschien et al., 2006;Campbell et al., 2006;Dhanasekaran et al., 2006;Tsui et al., 2006;Shearer et al., 2009), and the overall results of these studies suggest that the mitosporic ascomycetes are polyphyletic among ascomycetes, and this may be due to the conidia morphology, which reflects convergent or parallel evolution that has occurred as ascomycete species adapted (or preadapted) to freshwater habitats multiple times (Bärlocher, 2007). ...
The shift from terrestrial to freshwater ecosystems is accompanied by a dramatic shift in the decomposer fungal assemblages. The life cycle of freshwater fungi complicates our understanding of the phylogenetic relationships in this group. The freshwater ascomycetes (teleomorphs) often have asexual states (referred to as anamorphs or mitosporic states) in their life cycles. The asexual states of freshwater ascomycetes are often adapted to aquatic life by producing spores (conidia) that are long and filamentous or branched; this facilitates their attachment to substrates in moving water. Some species have conidia that are tightly coiled to trap air or are hydrophobic and hollow; these adaptations allow them to float on the surface of water until they attach to a new substrate. The main ecological functions
of freshwater fungi involve the breakdown of leaves, conifer needles, wood and other sources of coarse-particulate organic matter. Training students in this field is imperative given the increase in global transportation of microbes, the rapid loss of healthy freshwater habitats due to human perturbation and global warming, the serious problem of antibiotic resistance and the rarity of trained taxonomists and molecular systematists in the field of mycology.
