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Integrations in the HD-leptin-treated mouse liver DNA identified by RAIC PCR a Structure of the junction fragment in HD49. The open box indicates vector sequence from 49 to 1666; the bold line represents genomic sequence terminating with the mouse B1 element. b Integration HD72, with vector sequence from 72 to 1666 and the genomic with B1 element. c Integration HD220, with vector sequence from 220 to 1666 and the genomic with GT repeat. d, e Third round integration junction products identified by RAIC PCR: HD49, HD72, and HD220. Lanes M, molecular weight standards; lanes 1 and 4, 0.1 μg of control mouse DNA as control template; lanes 2 and 5, 5000 copies of HD-leptin vector spiked in 0.1 μg of mouse DNA were used as a nonintegrated assay controls; lanes 3, and 6, 0.1 μg of the HD-leptin-treated mouse liver DNA as template.
Source publication
While generally referred to as “non-integrating” vectors, adenovirus vectors have the potential to integrate into host DNA via random, illegitimate (nonhomologous) recombination. The present study provides a quantitative assessment of the potential integration frequency of adenovirus 5 (Ad5)-based vectors following intravenous injection in mice, a...
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Gene electrotransfer is an attractive method of non-viral gene delivery. However, the mechanism of DNA penetration across the plasma membrane is widely discussed. To explore this process for even larger structures, like viruses, we applied various combinations of short/long and high/low-amplitude electric pulses to L929 cells, mixed with a human ad...
Citations
... Any delivery of exogenous DNA into the nucleus is associated with heterologous recombination [10]. In rodent models, genome integration within intergenic regions of hepatocytes has been observed following administration of recombinant adenoviruses [11][12][13]. There have also been similar reports of tumorigenesis following adenoviral vaccination in rodents [14,15]. ...
... Persistent viral DNA exists in an extrachromosomal form as adenoviruses have no encoded capacity for integration and experimental estimates of integration rates of Ad5 put it at a median frequency of ~0.01% per transduced cell [54], [55]. None of these studies directly tested the impact of antigen persistence on the T cell responses induced by ChAdOx1 or Ad26. ...
Replication‐incompetent adenovirus (Ad) vector and mRNA‐lipid nanoparticle (LNP) constructs represent two modular vaccine platforms that have attracted substantial interest over the past two decades. Due to the COVID‐19 pandemic and the rapid development of multiple successful vaccines based on these technologies, there is now clear real‐world evidence of the utility and efficacy of these platforms. Considerable optimization and refinement efforts underpin the successful application of these technologies. Despite this, our understanding of the specific pathways and processes engaged by these vaccines to stimulate the immune response remains incomplete. This review will synthesize our current knowledge of the specific mechanisms by which CD8+ T cell and antibody responses are induced by each of these vaccine platforms, and how this can be impacted by specific vaccine construction techniques. Key gaps in our knowledge are also highlighted, which can hopefully focus future studies. This article is protected by copyright. All rights reserved
... Moreover, during the current pandemic, gene therapy was being discussed due to public concern regarding the effect of RNA and viral vector vaccines to alter the genome. Albeit most of the studies showed that those vaccines have no significant ability for genome integration, emerging studies showed other possibilities which need further investigation [27][28][29][30]. ...
Aim:
Gene therapy is expected to improve patients' quality of life. Medical students need to be aware about this technology as its application is becoming wider.
Materials & methods:
A web-based survey was conducted to measure the acceptance of Indonesian medical students regarding gene therapy.
Results:
Data from 621 valid responses showed that Indonesian medical students have little knowledge of this technology, with 34.4% of them ever heard of gene therapy. However, most of them support the approved gene therapy for health-related matters, but not on the non-health related matters. Their acceptance was determined by the sex, domicile and studentship status.
Conclusion:
Increasing medical students' knowledge of gene therapy is important to minimize the future conflict of gene therapy application.
V současné době existuje eminentní zájem států na proočkovanosti obyvatelstva proti COVID-19. V České republice k tomuto účelu slouží podmínečně registrované genové mRNA a vektorové DNA vakcíny, u kterých zatím absentují úplné a dlouhodobé údaje o bezpečn
