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Two new lamellar species, Camarophyllopsis olivaceogrisea and Hodophilus glaberripes , of the family Clavariaceae were discovered in the subtropical zone of China. Camarophyllopsis olivaceogrisea is morphologically characterized by its hygrophanous basidiomata, greenish gray to dull green pileus, shortly decurrent lamellae, broadly elliptic basidio...
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... generated sequences, related sequences used in previous studies (Adamčík et al. 2018) and a few sequences retrieved from GenBank by a Blast search were used to reconstruct phylogenetic trees. Detailed information on the newly sequenced samples, including the taxon names, voucher numbers, localities and GenBank accession numbers, is shown in Table 1. ITS and LSU sequences were respectively aligned using Clustal X v1.81 (Thomp- son et al. 1997) and manually modified where necessary in Bioedit v7.0.9 (Hall 1999), and a combined matrix of ITS and LSU sequences was obtained. The combined dataset was then analyzed using RAxML v7.2.6 (Stamatakis 2006) and MrBayes v3.1.2 ...Citations
Laccaria fengkaiensis and L. prava, collected from Heishiding Nature Reserve, South China, are proposed here as new taxa based on morphological and molecular evidences. These two new species along with Hodophilus glaberipes Ming Zhang et al. are described with photographs and line drawings and compared against related species. Morphologically, Laccaria fengkaiensis is characterized by a medium-sized to large pinkish white to light orange, strong striate to rugulose-striate or rugulose-sulcate pileus, a strong, solid stipe of the same color, 4-spored basidia, globose to obellipsoid basidiospores with moderate echinulae, and a pileipellis with abundant pileocystidia. Laccaria prava is characterized by a moderate-sized but very thin pinkish white to light orange, strong striate to rugulose-striate or rugulose-sulcate pileus, a distorted same colored stipe, 4-spored basidia, obellipsoid to globose basidiospores with moderate echinulae, and a stipitipellis with abundant caulocystidia. Hodophilus glaberipes is characterized by a small basidioma, with a pileus and its lamellae white at first, orange white to brownish orange with age, a white stipe, subglobose to globose basidiospores, and discoloring pastel red in wounds. Phylogenetic analysis of nucleotide sequences of the internal transcribed spacer (ITS) region provided further evidence that Laccaria fengkaiensis and L. prava are new species which are clearly separated from all other Laccaria species with rDNA sequence data available; Hodophilus glaberipes belongs to the H. micaceus superclade, with H. indicus K.N.A. Raj et al. as a closely related sister species.
