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Goose parvovirus (GPV) leads to a huge loss in the poultry industry, and early diagnosis is required to prevent the disease from spreading. At present, there are a variety of detection methods for GPV infection, and the ELISA method has the advantages of simple and rapid operation. However, most ELISA methods for detecting GPV can only detect the a...
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... Among these methods, ELISA for GPV antibody detection is widely used by laboratory personnel due to its ability to simultaneously test large batches of samples, its ease of operation, and its convenience. Reported ELISA methods for detecting antibody against GPV infection mainly include ELISA that utilize VP3 full-length protein as the coating antigen , ELISA that use VP3 antigen site VP3ep4-6 recombinant protein as the coating antigen (Tarasiuk et al., 2019), and ELISA that employ NS1 and VP3 fusion proteins for coating and can differentiate between natural infection and vaccine immunity (Zhang et al., 2020). These ELISA methods play a crucial role in the detection of antibody against GPV infection. ...
... When administering booster vaccinations to goslings in the later stages of their development, it's essential to conduct antibody testing in advance. This is because the presence of maternal antibodies in their system can impact the effectiveness of the vaccine-induced immunity (Fan et al., 2013;Zhang et al., 2020). Currently, in clinical practice, GPV antibody detection methods primarily involve agar gel diffusion tests and neutralization assays. ...
The detection of antibody against goose plague virus (GPV) infection has never had a commercialized test kit, which has posed challenges to the prevention and control of this disease. In this study, bioinformatics software was used to analyze and predict the dominant antigenic regions of the main protective antigen VP3 of GPV. Three segments of bovine serum albumin (BSA) vector-coupled peptides were synthesized as ELISA coating antigens. Experimental results showed that the VP3-1 (358-392aa) peptide had the best reactivity and specificity. By using the BSA-VP3-1 peptide, a detection method for antibody against GPV infection was established, demonstrating excellent specificity with no cross-reactivity with common infectious goose pathogen antibodies. The intra-batch coefficient of variation and inter-batch coefficient of variation were both less than 7%, indicating good stability and repeatability. The dynamic antibody detection results of gosling vaccines and the testing of 120 clinical immune goose serum samples collectively demonstrate that BSA-VP3-1 peptide ELISA can be used to detect antibody against GPV in the immunized goose population and has higher sensitivity than traditional agar gel precipitation methods. Taken together, the developed peptide-ELISA based on VP3 358-392aa could be useful in laboratory viral diagnosis, routine surveillance in goose farms. The main application of the peptide-ELISA is to monitor the antibody level and vaccine efficacy for GPV, which will help the prevention and control of gosling plague.
... 10 At present, many scholars at home and abroad have developed tests based on latex agglutination, colloidal gold immunochromatography, immunofluorescence technology, indirect enzyme-linked immunosorbent assay (ELISA), polymerase chain reaction (PCR) and other methods. [11][12][13] However, the widespread use of these methods was limited because it was necessary that precision instruments and a more elaborate method were detected the amplification products, although the target nucleic acids could be amplified within one hour. 14,15 The loop-mediated isothermal amplification (LAMP) is a novel nucleic acid amplification technique established by the Japanese scholar Notomi et al. 16 This new method has the advantages of simple operation, rapid response, low cost and visualization. ...
Gosling plague caused by goose parvovirus (GPV), a highly infectious septic disease with high mortality, has caused substantial loss in the waterfowl industry. A method for the rapid detection of GPV is needed. In this study, we isolated the virus strain of GPV in May 2020 and applied it to the loop-mediated isothermal amplification (LAMP) assay. We designed five sets of primers for the goose parvovirus VP3 gene by LAMP. The GV-1 primer set was selected to detect GPV sensitively and rapidly. LAMP was more sensitive compared to PCR. In addition, the LAMP method could complete detection within 60 min which was faster than the PCR assay. The LAMP provided a convenient and effective experimental method for detection of GPV for inspection and quarantine departments and health care units in China, and it is expected to become a simple and routine detection method, especially suitable for goose farms.
... The cutoff value was set to an OD of 0.459 at 450 nm. Each sample was tested thrice to ensure the accuracy of the test results (Zhang et al., 2020). ...
Since 2017, duck spleen necrosis caused by new variant duck orthoreovirus (N-DRV) infection had been observed in several provinces in China. This disease retards the growth and development of ducks, thereby reducing feed return rate. N-DRV infection causes damage to duck spleen and other immune organs, leading to immunosuppression and susceptibility to other pathogens. In this study, we successfully constructed a breeding duck artificial infection model and found that N-DRV infection can cause pathologic changes, such as ovarian hemorrhage, follicle atrophy, and fallopian tube bleeding in breeding ducks, resulting in significantly reduced fertilization rate and egg hatching rate. Viral RNA was present in egg vitelline membrane, duck embryo, and duckling’s spleen samples, as determined through quantitative polymerase chain reaction (qPCR). Autopsy revealed obvious pathologic changes in the spleen and other organs, although there were no obvious early clinical symptoms observed in ducklings. Sequence distance and phylogenetic analysis confirmed that N-DRV-SD19 re-isolated from the spleen samples of ducklings was consistent with the strain N-DRV-XT18 used for infecting breeding ducks. The findings in this study confirmed that N-DRV can be vertically transmitted through eggs, which provide an important reference for the disease prevention and control.
