Figure 3 - available via license: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
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Immunofluorescence micrograph of isolated 11.5 days embryonic yolk sac endothelial cells (3 days after passage) stained with vWF. (A and B: control), (C, D, E) different shape of endothelial cells (X400).
Source publication
Yolk sac blood islands are the first morphologic evidence of hematopoietic development during mammalian embryogenesis, and visseral yolk sac mesoderm gives rise to the first embryonic blood cells within a rich endothelial network. Present study reports the isolation and culture of endothelial cells from 11.5 days old embryonic rat yolk sac. The emb...
Contexts in source publication
Context 1
... primary cultures of 11.5 days old yolk sac endothelial cells were treated with anti vWF, a granular pattern of staining was observed in the perinuclear region (Fig. 3A, B-control) typical of that seen in endothelial cells from different species and organs (9,11). A variability of intensity of the reaction from cell to cell was noted in the primary culture. All cells exhibited some degree of specific staining when compared to controls. It was also noted that a single cell which has different shape showed a ...
Context 2
... cells from different species and organs (9,11). A variability of intensity of the reaction from cell to cell was noted in the primary culture. All cells exhibited some degree of specific staining when compared to controls. It was also noted that a single cell which has different shape showed a greater staining density than did groups of cells (Fig. 3C, D, ...
Citations
Labelling with endothelium specific monoclonal antibodies, von Willebrand Factor (vWF), rat endothelial cell antigen-1 (RECA-1), platelet-endothelial cell adhesion molecule-1 (PECAM-1), intercellular adhesion molecule-1 (ICAM-1), OX-43 and zonula occludentes-1 (ZO-1), was investigated in cryostat sections of vessels from rats of different ages using a confocal microscope. The results showed that labelling of the vWF was positive in endothelial cells from adult, fetal and different ages of embryonic rat. Labelling with RECA-1 was weakly positive in adult rat aorta and lung endothelial cells but not in embryonic yolk sac endothelial cells. Labelling using PECAM-1, ICAM-1 and OX-43 was negative in both adult and embryonic endothelial cells. ZO-1 showed positive but very weak reactivity in embryonic yolk sac endothelial cells. The expression of vWF on vessels from adult and 19.5-day fetal tissues was strongly positive. However, the expression of vWF in embryonic endothelial cells was dependent on the gestational age. While the 11.5-day yolk sac vessels stained weakly, staining gradually increased in 13.5-, 15.5- and 17.5-day-old yolk sac vessels. The results suggest that vWF is a reliable endothelial cell marker in rat vascular endothelial cells, including both fetal and embryonic stages.
