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Images of the EDS analyses: spreading from the ventral body surface to lateral plates. (a) Light microscope (LM). (b–e) Images from the EDS “mapping analysis” near application site no. 4 in (b), and EDS “line scanning analysis” (along yellow lines) of the same observation field (shown as insets) in (c–e). I–III indicate the positions analysed using EDS in (b). (f) LM. (g) Electron microscope (EM). (h) EDS “mapping analysis” images of characteristic fine structures with short protrusions in the lateral plate. Light blue signal indicates the localisations of elemental silica. A3–A6: abdominal spiracles (cf. Fig. 6a).

Images of the EDS analyses: spreading from the ventral body surface to lateral plates. (a) Light microscope (LM). (b–e) Images from the EDS “mapping analysis” near application site no. 4 in (b), and EDS “line scanning analysis” (along yellow lines) of the same observation field (shown as insets) in (c–e). I–III indicate the positions analysed using EDS in (b). (f) LM. (g) Electron microscope (EM). (h) EDS “mapping analysis” images of characteristic fine structures with short protrusions in the lateral plate. Light blue signal indicates the localisations of elemental silica. A3–A6: abdominal spiracles (cf. Fig. 6a).

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Numerous studies have focussed on the mechanisms of entry of pesticides into insect body parts such as oral intake, penetration through the integument of the body wall, and inhalation through spiracles. However, little is known about how insecticides spread to the points of entry or the paths on the body surface that are used to reach the target si...

Citations

... Various studies have been reported the entry of contact pesticides into insect body through oral consumption of treated foliage and fumigant pesticides through spiracles [9,10]. However, studies are limited with regard to the phytochemical pesticide penetration into insect body through chitinous insect cuticle [11]. Remarkably, Tak and Isman [12] reported the cuticular penetration as a mechanism of entry of plant volatiles as fumigant insecticide in insect pest. ...