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Identification of the major proteins of smooth muscle myofibrils by a gradient SDS PAGE. Left lane: turkey gizzard. Right lane: bovine trachea. The arrows on the right-hand side point to the individually labeled proteins. The empty arrowheads on the left-hand side point to the same proteins labeled with abbreviated names. In the right lane, calmodulin and caltropin overlap with the dye-front band.

Identification of the major proteins of smooth muscle myofibrils by a gradient SDS PAGE. Left lane: turkey gizzard. Right lane: bovine trachea. The arrows on the right-hand side point to the individually labeled proteins. The empty arrowheads on the left-hand side point to the same proteins labeled with abbreviated names. In the right lane, calmodulin and caltropin overlap with the dye-front band.

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Figure 1. Schematic diagram of the purification steps developed for...
Figure 2. Identification of the major proteins of smooth muscle...
Figure 3. Purification steps of bovine tracheal smooth muscle myosin...
Figure 4. Purification steps of bovine tracheal smooth muscle myosin...
+4 Figure 5. Purification steps of bovine tracheal smooth muscle myosin...
Purification of Myosin from Bovine Tracheal Smooth Muscle, Filament Formation and Endogenous Association of Its Regulatory Complex
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  • Feb 2023
Dynamic regulation of myosin filaments is a crucial factor in the ability of airway smooth muscle (ASM) to adapt to a wide length range. Increased stability or robustness of myosin filaments may play a role in the pathophysiology of asthmatic airways. Biochemical techniques for the purification of myosin and associated regulatory proteins could hel...
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Context 1
... predominant or readily identified species in the extracted smooth muscle crude actomyosin are, besides myosin with its regulatory (Re) and essential (Es) light chains (LCs), actin and the actin-binding proteins tropomyosin (TM), α-actinin, and filamin. These and the other identifiable proteins are shown in Figure 2. Other proteins in the crude actomyosin extract need further purification steps to be identified as bands on the gel. ...