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Objective: Pharmacognostical study along with the development of a quantitative HPTLC method for Crinum latifolium and evaluation of its traditional claims.Methods: Quantification of three marker compounds oleanolic acid, linoleic acid, and lupeol was done through HPTLC. In vitro antioxidant activity was determined by six different models, namely t...
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... The antidiabetic assay was performed as per the method used by Shukla et al. [22], using the 3,5-Dinitrosalicylic acid method. The absorbance of reaction mixtures was then measured at 540 nm and percentage inhibition was calculated and A c and A s absorbance of control and sample, respectively. ...
In this study, the effect of dip blanching-DB, steam blanching-SB (2 and 4 min), and microwave blanching-MB (180, 450, and 750 W) on the physicochemical, functional, bioactive, and structural characteristics of okra were studied. Compared with the unblanched sample, blanching led to a significant change in the color and water activity of okra. The results regarding functional properties indicated that water and oil absorption capacity and rehydration ratio improved during MB and samples showed the shear thinning behaviour as the shear rate increased. The retention of bioactive compounds following the trend of MB > SB > DB. Fourier-transform infrared spectroscopy also confirms the maximum retention of phytocompounds in MB. The scanning electron microscopy and X-ray diffraction revealed that the blanched sample had a porous, and less crystalline surface, which was responsible for changes in functional properties. The results are suggestive that MB okra powder has potential to serve as a techno-functional food ingredient for industrial applications.
... High-performance thin layer chromatography analysis of aerial parts and root showed the presence of oleanolic acid, linoleic acid, and lupeol (Shukla et al., 2018). Highest oleanolic acid, linoleic acid, and lupeol were found in aerial part of C. latifolium (0.015, 0.048, and 0.028%, respectively). ...
... Among the isolated compounds, 9-methoxycripowellin B and 4-methoxy-8-hydroxy-cripowellin B revealed promising antioxidant activities (IC 50 < 0.50 mM). Shukla et al. (2018) studied free radical scavenging activity by DPPH assay and showed the promising antioxidant activity from the roots and aerial parts (IC 50 values of 410±1.105 and 441.95±1.788 ...
... and 193.33±2.45 µg/mL, respectively) as reported by Shukla et al. (2018). Similarly, crude leaf extract (300 μg) showed the highest (66.10%) percent inhibition of α-glucosidase enzyme as compared to α-amylase (Ghane et al., 2018). ...
... High-performance thin-layer chromatography (HPTLC) analysis results of the E MeOH obtained from the aerial parts and roots of the plant led to the identification of two pentacyclic triterpenes, oleanolic acid (66, 0.006 % and 0.015 % dry weight basis, respectively) and lupeol (64, 0.026 % and 0.029 % dry weight basis, respectively), and one fatty acid, linoleic acid (65, 0.027 % and 0.048 % dry weight basis, respectively). These compounds were characterized as markers for the E MeOH of C. latifolium (Shukla et al., 2018). Furthermore, reverse phase high performance liquid chromatography (RP-HPLC)based quantification of phenolic compounds in the E MeOH from leaves yielded 3519 mg of hydroxybenzoic acid (56)/g of extract. ...
... Parameters such as total phenol content (TPC), total flavonoid content (TFC), total tannin content (TTC), and total alkaloid content (TAC) have been estimated in different studies. TPC in the E MeOH from aerial parts and roots was calculated as 2.32 and 3.59 mg gallic acid equivalents/g of extract, respectively, and the TFC in the E MeOH from aerial parts and roots was reported to be 0.75 and 0.92 mg quercetin equivalents/g of extract, respectively (Shukla et al., 2018). TPC in the E MeOH from leaves was calculated as 17.5 mg gallic acid equivalents/g of extract (Aziz et al., 2014a). ...
... Ghane et al. (2018) estimated the AA in extracts from the leaves of C. latifolium and found values of »40 and 55 mg TE/g of extract in the DPPH assay; »40 and 20 mg TE/g of extract in the ABTS assay; »225 and 275 mg Fe(II)/g of extract in the FRAP assay; and »45 and 90 mg ascorbic acid equivalent (AAE)/g of extract in the ABTS assay for E aq and E MeOH , respectively. IC 50 values of 442 and 410 mg/mL were reported for the E MeOH obtained from the aerial parts and roots of C. latifolium, respectively (Shukla et al., 2018). Nguyen et al. (2013) determined the AA of E aq , E EtOH , and alkaloid extract (E alk ) prepared from the leaves of C. latifolium. ...
Crinum latifolium L. (Amaryllidaceae) is an herbaceous ornamental species that grows throughout the tropics and warm temperate regions, mainly in the Americas, Australia, and Southern Asia. Several phytochemical and biological studies on this species have been conducted, and pharmacological studies have revealed its promising anti-inflammatory, antioxidant, and anticancer properties using different in vitro and in vivo models. The medicinal properties of this species have been attributed to the presence of several secondary metabolites (e.g., alkaloids). In C. latifolium bulbs, lycorine, hemanthamine, tazettine, and crinine-type alkaloids have been identified with diverse chemical structures. Furthermore, crinine, hemanthamine, and tazettine-type alkaloids are the most abundant in leaves. The present review aims to summarize research on the phytochemical and pharmacological properties of C. latifolium. As the scientific data indicate promising medicinal applications, this review will help researchers working in the fields of pharmacognosy, natural products, and health and related sciences to focus on further studies that yield concrete evidence regarding the potential of C. latifolium extracts for the treatment of different human disorders.
... The present study showed that limitation in the intestinal lipid absorption, prevention of angiogenesis, decrease in inflammatory mediators, and alteration of oxidative parameters is the potential pathway by which the Crinum latifolia leaf extract prevented gain in body weight in the HFD-fed rats. It may be possible that the presence of hypolipidemic compounds in the extract may influence the lipid dynamics and moreover prevents the obesity development in treated rats (Itoh et al. 2009;Shukla et al. 2018;Horrobin and Huang 1987). The HFD causes increased lipogenesis which leads to the upregulation of plasma lipids such as TG and LDL levels and reduced serum HDL in obese rats (Kutoh and Yamashita 2012). ...
Obesity is a multifaceted disease encompassing deposition of an unnecessary amount of fat which upsurges the possibility of other complications, viz., hypertension and certain type of cancers. Although obesity results from combination of genetic factors, improper diet and inadequate physical exercise also play a major role in its onset. The present study aims at exploring the anti-obesity activity of Crinum latifolia leaf extract in obese rats. The leaves were extracted using hydroalcoholic extraction which was later diluted with water and given to obese rats. The dosing was started from the 4th week (by oral administration of extract of Crinum latifolia (100 mg/kg and 200 mg/kg) and combination of Crinum latifolia leaf extract 200 mg/kg and orlistat 30 mg/kg) till the 10th week. Various angiogenic, antioxidant, biochemical, and inflammatory biomarkers were assessed at the end of the study. The obese symptoms were progressively reduced in treatment groups when compared to disease control groups. The angiogenic parameters and inflammatory parameters were consequently reduced in treatment groups. The oxidative parameters superoxide dismutase (SOD) and catalase were gradually increased, while levels of TBARS were reduced in treatment groups showing antioxidant nature of leaf hydroalcoholic extract. The Crinum latifolia leaf extract possesses anti-obesity properties and therefore can be used as a therapeutic option in the management of obesity.
Crinum latifolium , Eclipta prostrata , Ocimum gratissimum , Phyllanthus urinar ia and Streptocaulon juventas have been used in Vietnam traditional medicine practices for anti-inflammatory, anti-cancer effects and antioxidant activities since prehistoric times. Ethanol and aqueous extracts from five medicinal herbs were evaluated for their antioxidative, free radical scavenging activities and phytochemical screening. Research results showed that these medicinal herbs all had rich chemical composition, such as alkaloids, coumarins, flavonoids, phenols, etc. Among ten extracts, C.l atifol ium ethanol extract showed the significantly highest total phenolic content (66.8 ± 4.14 mgGAE/g) and total flavonoids content (99.69 ± 9.88 mgQE/g). Both ethanol extracts from O.gratissimum and P.urinaria were showed the most elevated antioxidant potentials in DPPH and ABTS assays. These findings suggest that the rich phytochemical content and the good antioxidant activity of five herbal plants may be responsible for its popular and wide traditional use.
The commercialization of medicinal plants as recognized in their uses by nutraceutical, cosmeceutical and pharmaceutical industries is gaining more popularity and interest around the globe. The plant family Amaryllidaceae has a long history in the traditional medicinal system and used to treat diverse diseases. It is well known for its structurally-diverse alkaloids which exhibit a wide range of pharmacological activities. Crinum, an important bulbous geophyte is well known for its ornamental and therapeutic properties. In India, Crinum is represented by 15 different species which contain several alkaloids with biological properties. However, some of the medicinal uses of Indian Crinum species still require pharmacological analysis. In addition, more extensive research including different biotechnological approaches is needed in order to guarantee its sustainable utilization and long-term conservation. Further research is essential to improve our current knowledge about the bioactive components, toxicity, clinical relevance as well as its conservation status. The present review summarizes the research that has been done on the phytochemistry, pharmacology and in vitro studies of the Indian Crinum. Potential toxicity aspects as well as knowledge gaps and future perspectives are also discussed.
