Fig 4 - uploaded by Stephen Murphy
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Hyaluronan synthase transfection as demonstrated by red blood cell (RBC) exclusion. (a) Control. Note the RBC cover tendon fibroblasts. (b) RBC exclusion in a halo around transfected cells. (c) Halo effect abolished by treatment with hyaluronidase. (All ×400.)
Source publication
The objective of this paper was to study in vitro transfection of tendon cells and adherence of transfected cells to different tendon surfaces. Achilles tendon fibroblasts from 2-month-old New Zealand white rabbits were cultured to confluence, after which the cells were transfected by an adenovirus carrying either the β-galactosidase reporter gene...
Context in source publication
Context 1
... by these results, we attempted the transfection of a functional gene, mouse hyaluronan synthase. Preliminary data using a red blood cell exclusion assay suggests that we have been able to transfect tendon cells successfully with this gene, and that hyaluronate is produced after transfection (Fig. ...
