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Homologous VQs across Arachis species. A Orthologous and paralogous VQs gene pairs across Arachis duranensis, A. ipaensis, and A. monticola. B Orthologous and paralogous VQs gene pairs across A. duranensis, A. ipaensis, and A. hypogaea cv. Fuhuasheng. C Orthologous and paralogous VQs gene pairs across A. duranensis, A. ipaensis, and A. hypogaea cv. Shitouqi. D Orthologous and paralogous VQs gene pairs across A. duranensis, A. ipaensis, and A. hypogaea cv. Tifrunner. Blue and red lines indicate paralogous and orthologous VQs. Aradu, Araip, A/B, Ahy, Chr, and Arahy indicate chromosomes in A. duranensis, A. ipaensis, A. monticola, A. hypogaea cv. Fuhuasheng, A. hypogaea cv. Shitouqi, and A. hypogaea cv. Tifrunner, respectively
Source publication
Valine-glutamine sequences (VQs) interact with WRKY transcription factors (TFs), forming VQ-WRKY protein complexes crucial for plant development and response to environmental changes. Cultivated peanut (Arachis hypogaea) is a tetraploid from A. duranensis and A. ipaensis cross. The Arachis spp. WRKY TFs have been identified, but Arachis VQs are lar...
Citations
... For instance, the overexpression of the Arabidopsis CBF1/DREB1B or CBF3/DREB1A genes enhanced tolerance to salt, drought, and other abiotic stresses (Maruyama et al. 2004;Jaglo-Ottosen et al. 1998;Liu et al. 1998;Lee et al. 2003). It was reported that MdCER1-1, MT1, and valine-glutamine genes play a vital role in drought stress (Gao et al. 2023;Zhang et al. 2022;Kumar et al. 2022). It has been reported that the HKT1;5 gene is vital for improving salt stress in rice by maintaining the Na + /K + ratio (Shohan et al. 2019). ...
MATH-BTB proteins are involved in a variety of cellular processes that regulate cell homeostasis and developmental processes. Previous studies reported the involvement of BTB proteins in the development of various organs in plants; however, the function of BTB proteins in salt stress is less studied. Here, we found a novel MATH-BTB domain-containing OsMBTB32 protein that was highly expressed in leaf, root, and shoot. The up-regulation of the OsMBTB32 transcript in 2-week-old seedlings under salt stress suggests the significant role of the OsMBTB32 gene in salinity. The OsMBTB32 transgenic seedlings (OE and RNAi) exhibited significant differences in various phenotypes, including plumule, radical, primary root, and shoot length, compared to WT seedlings. We further found that OsCUL1 proteins, particularly OsCUL1-1 and OsCUL1-3, interact with OsMBTB32 and may suppress the function of OsMBTB32 during salt stress. Moreover, OsWRKY42, a homolog of ZmWRKY114 which negatively regulates salt stress in rice, directly binds to the W-box of OsCUL1-1 and OsCUL1-3 promoters to promote the interaction of OsCUL1-1 and OsCUL1-3 with OsMBTB32 protein in rice. The overexpression of OsMBTB32 and OsCUL1-3 further confirmed the function of OsMBTB32 and OsCUL1s in salt tolerance in Arabidopsis. Overall, the findings of the present study provide promising knowledge regarding the MATH-BTB domain-containing proteins and their role in enhancing the growth and development of rice under salt stress.
MATH-BTB proteins are involved in a variety of cellular processes that regulate cell homeostasis and developmental processes. Previous studies reported the involvement of BTB proteins in the development of various organs in plants; however, the function of BTB proteins in salt stress is less studied. Here, we found a novel MATH-BTB domain-containing OsMBTB32 protein that was highly expressed in leaf, root, and shoot. The up-regulation of the OsMBTB32 transcript in 2-week-old seedlings under salt stress suggests the significant role of the OsMBTB32 gene in salinity. The OsMBTB32 transgenic seedlings (OE and RNAi) exhibited significant differences in various phenotypes, including plumule, radical, primary root, and shoot length, compared to WT seedlings. We further found that OsCUL1 proteins, particularly OsCUL1-1 and OsCUL1-3, interact with OsMBTB32 and may suppress the function of OsMBTB32 during salt stress. Moreover, OsWRKY42, a homolog of ZmWRKY114 which negatively regulates salt stress in rice, directly binds to the W-box of OsCUL1-1 and OsCUL1-3 promoters to promote the interaction of OsCUL1-1 and OsCUL1-3 with OsMBTB32 protein in rice. The overexpression of OsMBTB32 and OsCUL1-3 further confirmed the function of OsMBTB32 and OsCUL1s in salt tolerance in Arabidopsis. Overall, the findings of the present study provide promising knowledge regarding the MATH-BTB domain-containing proteins and their role in enhancing the growth and development of rice under salt stress.
... Tifrunner genomes have been used to identify gene families such as nucleotide-binding site-leucinerich repeat (NBS-LRR), LRR-containing genes, and heat shock transcription factor (HSF) [24][25][26]. To our knowledge, only the valine-glutamine (VQ) gene family has been compared among the above-mentioned six Arachis genomes [27]. The study found that the VQs increased in A. monticola, A. hypogaea cv. ...
... Fuhuasheng, and A. hypogaea cv. Shitouqi compared to A. duranensis and A. ipaensis [27]. ...
... WRKY TFs are auto-and cross-regulated by the W-box cis-acting elements [27,39,40]. In this study, the 2-kb upstream sequences of WRKY genes were extracted using the genetic feature format (GFF) by the TBtools program [41]. ...
Background
Cultivated peanut ( Arachis hypogaea ), a progeny of the cross between A. duranensis and A. ipaensis , is an important oil and protein crop from South America. To date, at least six Arachis genomes have been sequenced. WRKY transcription factors (TFs) play crucial roles in plant growth, development, and response to abiotic and biotic stresses. WRKY TFs have been identified in A. duranensis , A. ipaensis , and A. hypogaea cv. Tifrunner; however, variations in their number and evolutionary patterns across various Arachis spp. remain unclear.
Results
WRKY TFs were identified and compared across different Arachis species, including A. duranensis , A. ipaensis , A. monticola , A. hypogaea cultivars (cv.) Fuhuasheng, A. hypogaea cv. Shitouqi, and A. hypogaea cv. Tifrunner. The results showed that the WRKY TFs underwent dynamic equilibrium between diploid and tetraploid peanut species, characterized by the loss of old WRKY TFs and retention of the new ones. Notably, cultivated peanuts inherited more conserved WRKY orthologs from wild tetraploid peanuts than their wild diploid donors. Analysis of the W-box elements and protein–protein interactions revealed that different domestication processes affected WRKY evolution across cultivated peanut varieties. WRKY TFs of A. hypogaea cv. Fuhuasheng and Shitouqi exhibited a similar domestication process, while those of cv. Tifrunner of the same species underwent a different domestication process based on protein–protein interaction analysis.
Conclusions
This study provides new insights into the evolution of WRKY TFs in Arachis spp.
