Figure 2 - uploaded by Nasrin Sultana
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Histological images of the subcutaneous adipose tissue from non-DEX (DEX at 0 mg/kg feed), DEX-1 (DEX at 3 mg/kg feed), DEX-2 (DEX at 5 mg/kg feed), and DEX-3 (DEX at 7 mg/kg feed) groups of broilers. Hematoxylin-eosin staining. A -Adipocytes. Actual magnification 400×; scale bar = 100 μm.
Source publication
Objective: The objective of this investigation was to determine the effects of dexamethasone (DEX) on the weight and cellularity of abdominal and subcutaneous fat depots.
Materials and Methods: The study was conducted on four broiler chicks (20 chicks per group) fed commercial feed and water ad libitum. The DEX was supplied with feed at 0 mg/kg (n...
Citations
... To study the microscopic characteristics of the cryodehydrated specimens, approximately a 0.5-square-cm sample was taken from each specimen (both fresh and cryodehydrated) and processed for routine staining (Harris hematoxylin and eosin stain) following the standard protocol [21]. The histomorphological and histomorphometric characteristics (i.e., the thickness of the heart wall, the circumference of the bronchi and bronchioles, the surface area of lung alveoli, the circumference of the hepatic central veins (CVs) of the liver and renal tubules (RT), the glomerular surface area, and the cross-sectional area of the MF) were investigated using a photomicroscope (Model: B-293, Optika, Italy). ...
Objective
This study sought to determine the effectiveness of the cryodehydration technique in preserving the morphologic and morphometric attributes of the anatomical specimens of goats.
Materials and methods
Different anatomical parts of a goat, i.e., heart, lungs, spleen, liver, kidney, and musculoskeletal specimens, were collected and fixed in 15% formalin. Later on, the fixed specimens were cryodehydrated by fast freezing (burning process) and repeated freezing-thawing sessions, followed by wood glue coating. Finally, the macroscopic (i.e., weight, color, texture, odor, and durability) and microscopic characteristics (by routine hematoxylin and eosin staining) of the cryodehydrated specimens were studied.
Results
The resultant specimens produced excellent color and texture and were lightweight (60%–80% weight loss), soft, dry, odorless, durable, and easy to handle. The histoarchitectural details of the heart and skeletal muscle were well preserved, while some distinctive alterations were observed in the parenchymatous organs, i.e., breach in cellular integrity, loss of cell cytoplasm, loss of cytoplasmic and nuclear clarity, increased sinusoidal space, dilatation of the renal tubules, and reduction in glomerular size. Nevertheless, the basic histoarchitecture of each specimen was yet to be distinctly identifiable.
Conclusion
The current study findings suggest that the cryodehydration technique can preserve gross anatomical features as well as histoarchitectural details and can be an effective tool for facilitating veterinary education and research.
