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Histological assessment of cyst wall tissue. The HE staining (a) and Masson staining (b) of cyst wall tissue show the presence of endothelial cells of blood vessels in popliteal cyst. Immunohistochemical staining of CD31 (c) and vWF (d) showed positive expression. Immunohistochemical staining of inside wall tissue of popliteal cyst cavity showed negative expression of AE1/3 (e). Scale bars=200 μm.
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Mesenchymal stem cells (MSCs) are multipotent progenitor cells in adult tissues. The aim of this study is to isolate and identify synovial fluid-derived mesenchymal stromal cells (SF-MSCs) from the popliteal cyst fluid of pediatric patients. SF-MSCs were collected from the popliteal cyst fluid of pediatric patients during cystectomy surgery. After...
Citations
... OA, a degenerative disease characterized by cartilage injury, the contributory factors include ag, joint injury, and femoral acetabular impact shape [32]. One study showed that the number of SF-MSCs increased with cartilage degeneration and the severity of osteoarthritic disease compared to healthy controls [19].During OA progression, CD44 expression gradually increases [33]. of synovial tissue or intra-articular injection of digestive factors [39]. Tissue resection can cause joint trauma, and similarly, intra-articular injection of digestive factors can damage SM-MSCs, whereas SF-MSCs can be obtained by arthrocentesis, which is less invasive and more widely used in outpatient settings than the previous two methods. ...
Synovial membrane mesenchymal stem cells (SM-MSCs), a type of tissue-specific stem cells, are involved in forming synovial joints in embryos and the maintenance and repair of joint tissue in adults. Mesenchymal stem cells (MSCs) produced from synovial fluid (SF-MSCs) may have therapeutic properties with a higher capacity for cartilage proliferation than other MSC types.The research domains of SM-MSCs and SF-MSCs, however, have not been subjected to bibliometric analysis.This study aimed to visualize and analyze their research areas and trends using a bibliometric approach. Most studies on SM-MSCs and SF-MSCs are focused on basic research, but as SM-MSCs play important roles in chondrogenesis, osteogenesis, myogenesis, and tendon formation, they may have therapeutic potential for cartilage and tissue repair linked to diseases. The current study is the first to thoroughly introduce the development patterns and hotspots in this field of study.
... Next, we differentiated FOXO1 +/+ and FOXO1 -/-hESCs into synoviocyte-like human mesenchymal stromal cells (hMSCs). These hMSCs displayed the typical markers of MSCs and exhibited the expression of a specific set of genes associated with fibroblast-like synoviocytes ( Fig. S5A) (Li et al., 2020a;Li et al., 2019;Li et al., 2020b), thereby serving as a cellular model to investigate the functional and molecular regulations in synoviocytes. We assessed the expression of FOXO1 in FOXO1 +/+ hMSCs at both early (EP) and late passages (LP). ...
The synovium, a thin layer of tissue that adjacent to the joints and secretes synovial fluid, undergoes changes in aging that contribute to intense shoulder pain and other joint diseases. However, the mechanism underlying human synovial aging remains poorly characterized. Here, we generated a comprehensive profile of synovial cell types present in subacromial synovium from young and aged individuals. By delineating aging-related transcriptomic changes across cell types and their associated regulatory networks, we identified two subsets of mesenchymal stromal cell (MSC) in human synovium, which are lining and sublining MSCs, and found that angiogenesis and fibrosis-associated genes were upregulated whereas genes associated with cell adhesion and cartilage development were downregulated during aging. Moreover, the specific cell-cell communications in aged synovium mirrors that of aging-related inflammation and tissue remodeling, including vascular hyperplasia and tissue fibrosis. In particular, we identified Forkhead box O1 (FOXO1) as one of the major regulons for aging DEGs of synovium MSCs, and validated its downregulation in both lining and sublining MSC populations of the aged synovium. In human FOXO1-depleted MSCs derived from human embryonic stem cells, we recapitulated the senescent phenotype observed in the subacromial synovium of aged donors. These data indicate the important role for FOXO1 in the regulation of human synovial aging. Overall, our study improves upon our understanding of synovial aging during joint degeneration, thereby informing development of new treatments aimed at rejuvenating the aged joint.
... The therapeutic potential of UDSCs for neurological diseases remains relatively unexplored, yet the limited findings obtained thus far hold promise. UDSCs engraftment or their secretomes have shown to be effective mostly in preclinical reports of renal and bladder diseases, such as acute kidney injury , renal acute tubular injury (Grange et al., 2020) or bladder fibrosis (Wu et al., 2023), evidencing a consistent tissue regeneration effect, mainly through their pro-angiogenic, anti-apoptotic and antiinflammatory effects (Grange et al., 2020;Li F. et al., 2020;Wu et al., 2023, over the living afflicted tissues. Within the field of neurological disorders, very few studies of UDSCs in vivo preclinical engraftment have been performed. ...
Stem cells have potential applications in the field of neurological diseases, as they allow for the development of new biological models. These models can improve our understanding of the underlying pathologies and facilitate the screening of new therapeutics in the context of precision medicine. Stem cells have also been applied in clinical tests to repair tissues and improve functional recovery. Nevertheless, although promising, commonly used stem cells display some limitations that curb the scope of their applications, such as the difficulty of obtention. In that regard, urine-derived cells can be reprogrammed into induced pluripotent stem cells (iPSCs). However, their obtaining can be challenging due to the low yield and complexity of the multi-phased and typically expensive differentiation protocols. As an alternative, urine-derived stem cells (UDSCs), included within the population of urine-derived cells, present a mesenchymal-like phenotype and have shown promising properties for similar purposes. Importantly, UDSCs have been differentiated into neuronal-like cells, auspicious for disease modeling, while overcoming some of the shortcomings presented by other stem cells for these purposes. Thus, this review assesses the current state and future perspectives regarding the potential of UDSCs in the ambit of neurological diseases, both for disease modeling and therapeutic applications.
The present study investigated whether bone marrow-derived mesenchymal stem cells (BMMSCs) facilitate angiogenesis and improve outcomes of pregnancy with obstetric deep venous thrombosis (DVT) and explored the underlying mechanism. A pregnant DVT rat model was established using a "stenosis" method on the lower segment of the inferior vena cava (IVC). The extent of vascularization in thrombosed IVC was examined by immunohistochemistry. In addition, the effect of BMMSCs on DVT pregnancy outcomes was evaluated. We also characterized the effect of BMMSC-derived conditioned medium (BM-CM) on the impaired human umbilical vein endothelial cells (HUVECs). Thereafter, transcriptome sequencing was employed to identify the differentially expressed genes in thrombosed IVC tissues of DVT and DVT plus BMMSCs (thrice) groups. Lastly, the candidate gene's role in the promotion of angiogenesis was demonstrated in vitro and in vivo. The DVT model was successfully established using IVC stenosis. The injection of three consecutive BMMSC doses into pregnant SD rats with DVT was demonstrated to be the most effective treatment, which significantly reduced the length and weight of the thrombus, induced the highest level of angiogenesis, and ameliorated the embryo absorption rate. In vitro, BM-CM efficiently increased the abilities of impaired endothelial cells to proliferate, migrate, invade, and form vessel-like tubes, while inhibiting their apoptosis. Transcriptome sequencing revealed that BMMSCs induced a prominent upregulation of a variety of pro-angiogenic genes, including secretogranin II (SCG2). When SCG2 expression was knocked down by lentivirus, the BMMSCs' and BM-CM-induced pro-angiogenic effects on pregnant DVT rats and HUVECs were markedly attenuated. In conclusion, the study results suggest that BMMSCs enhance angiogenesis via up-regulation of SCG2, providing an effective alternative regenerative agent and novel target for the therapy of obstetric DVT.
