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Hierarchical cluster analysis of 32 H. cordata leaves samples and four powder products. All samples were classified into nine clusters labeled A-G.
Source publication
Chemical profiling for quality monitoring and evaluation of medicinal plants is gaining attention. This study aims to develop an HPLC method followed by multivariate analysis to obtain HPLC profiles of five specific flavonoids, including rutin (1), hyperin (2), isoquercitrin (3), quercitrin (4), and quercetin (5) from Houttuynia cordata leaves and...
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An ethnobotanical study was carried out among the peoples of the Kaibarta Community of Upper Brahmaputra Valley Zone of Assam to assess their rich indigenous knowledge system, regarding the application of the ethnomedicinal plants as phyto remedies against certain common ailments. The information on the application of medicinal plants was obtained...
Houttuynia Cordata (HC) is a widely distributed plant in Asia and is used extensively for both food and medicinal purposes. A preliminary investigation found that HC is often bleached with sodium metabisulfite solution during its field processing, leading to health risks. In this study, the effects of sodium metabisulfite on the quality of HC were...
The biological synthesis of functionalised nanoparticles using plant materials is considered more cost‐effective and eco‐friendly than chemical and physical methods. Hence, this study demonstrated the synthesis of silver nanoparticles (AgNPs) using Houttuynia cordata Thunb rhizome (HCR). The extract, prepared with distilled water, provided the requ...
Citations
... Two main flavonoids in H. cordata extract, including hyperin and quercitrin, were evaluated using calibration curves that have been previously published [14]. ...
Background
Houttuynia cordata Thunb. has long been widely used as a daily vegetable and traditional medicine. The flavonoid component of H. cordata has plenty of pharmacological effects, such as antibacterial, anti-inflammatory, and antioxidant. In this study, we applied the aqueous two-phase system (ATPS) combined with ultrasonic extraction for extracting H. cordata leaves.
Methods
We optimized the extraction process to improve the extraction efficiency of the two flavonoids, hyperin and quercitrin, by Surface Method Response - Central Composite Design (RSM-CCD). Next, we investigated the antibacterial ability of H. cordata ATPS extract from optimal conditions against two bacterial strains, Cutibacterium acnes and Staphylococcus epidermidis.
Results
The results showed that using 10% (NH4)2SO4 and 35% ethanol for ATPS extraction resulted in the highest hyperin and quercitrin contents. From the RSM-CCD results, the optimal extraction conditions were determined to be ultrasonic extraction at 50 °C for 30 min, giving results consistent with the predicted model and obtaining hyperin and quercitrin contents at 1.5681 ± 0.0114 and 4.6225 ± 0.0327 mg/g, respectively.
Furthermore, ATPS extract has excellent antibacterial activity with a minimum inhibitory concentration (MIC) value of 250 μg/mL on both C. acnes and S. epidermidis. This MIC is significantly lower than the H. cordata ultrasound-assisted (UA) extract, with MICs of 1500.00 and 156.25 μg/mL on C. acnes and S. epidermidis, respectively. In addition, the results from the disk diffusion assay also showed that ATPS extraction has superior internal antibacterial activity with a zone of inhibition diameter at 250 μg/mL of 8.67 ± 1.15 and 5.00 ± 2.00 mm. Meanwhile, those of UA extract on C. acnes is 5.67 ± 1.53 mm (at 1500 μg/mL), and on S. epidermidis is 1.34 ± 0.58 mm (at 156.25 μg/mL).
Conclusion
To sum up, our research highlights the potential of H. cordata ATPS extracts as the starting material for topical preparations for effectively treating acne.
... The name of the method means that it combines two approaches -chromatographic purification of the total or individual flavonoids and subsequent spectrophotometric determination of the target substances. The chromatospectrophotometric method can be carried out in various modifications, but in general they can be divided into 2 groups: 1. Chromatographic separation of flavonoids from accompanying substances by TLC or HD (for example, determination of rutin in medicinal plants) [33,34]. Methods for the quantitative determination of rutin in buckwheat grass and Sophora japonica buds are based on chromatospectrophotometry, and in the first case, paper chromatography is used, and in the second, TLC. ...
Flavonoids are active metabolites of plant cells. The significant biological role of these compounds is evidenced by the nature of their distribution in the plant. Flavonoids play an important role in plant reproduction, development and accumulation of pollen, and their content also determines the color of flowers, fruits, and plant seeds. Most flavonoids are found in actively functioning organs: leaves, flowers, fruits (color, aroma), seedlings, as well as in integumentary tissues that perform protective functions. Different organs and tissues differ in the quantity and qualitative composition of flavonoids. Relatively recently, it was found that flavonoids also influence signaling processes occurring in living systems due to specific interactions with proteins that perform regulatory functions. Numerous data have been accumulated on the influence of these compounds on other protein and non-protein structures, which can lead to changes in the functional state of cells and the entire organism as a whole. Despite the fact that currently the redox properties of phenolic compounds are not given so much importance, their comprehensive study still remains an important task. In addition, numerous studies confirm that flavonoids act as effective phytoalexins, exhibiting a wide range of antibacterial and antifungal activities. After all, a significant role of flavonoids is their function of protecting against various unfavorable factors for plants, such as temperature fluctuations, the influence of ultraviolet rays, attacks by viruses, bacteria, and parasites. A correlation has been established between their content in plants and plant resistance to fungal diseases and some pests. The appearance of these compounds in plants in response to the action of parasitic fungi of cultivated plants and inhibition of the growth of fungal mycelium is considered a protective reaction. Flavonoids with antimicrobial activity are found in large quantities in the seed coat. This review paper highlights the basic technologies for extraction and identification of flavonoids from different type of plants.
