Figure 4 - uploaded by Per Sandgren
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Hemostatic properties of cryopreserved platelets are affected by the platelet concentration. Thromboelastometry was performed to estimate the hemostatic function of cryopreserved platelets. Panels display (A) clotting time, (B) clot formation time, and (C) maximal clot firmness. DMSO-free platelets showed no significant difference compered to DMSO regarding CT and CFT, but slightly reduced maximal clot firmness. However, when the DMSO-free products were reconstituted in less plasma to a higher platelet concentration, all three parameters were affected. p-values are defined as * p < 0.05 and *** p < 0.001. Error bars: 95% CI.
Source publication
Dimethyl sulfoxide (DMSO) is regularly used as a cryoprotectant agent for the cryopreservation of platelets. However, DMSO is considered toxic. We therefore hypothesized that saline could be used as a non-toxic medium for the cryopreservation of platelets. Double-dose buffy coat platelets (n = 10) were divided and cryopreserved at −80 °C using 5–6%...
Contexts in source publication
Context 1
... 102.6 (p = 0.175), respectively. However, the maximal clot firmness was significantly (p = 0.034) higher in the DMSO group, with a mean of 31.5 ± 3.5 mm, compared to the DMSO-free group, with a mean of 28.0 ± 3.6 mm ( Figure 4). Mitochondrial membrane potential (JC-1+) was measured to estimate platelet viability in fresh, DMSO-containing, and DMSO-free cryopreserved platelets. ...
Context 2
... 102.6 (p = 0.175), respectively. However, the maximal clot firmness was significantly (p = 0.034) higher in the DMSO group, with a mean of 31.5 ± 3.5 mm, compared to the DMSO-free group, with a mean of 28.0 ± 3.6 mm ( Figure 4). Thromboelastometry was performed to estimate the hemostatic function of cryopreserved platelets. ...
Context 3
... showed that all three parameters were significantly affected by the added volume of plasma, indicating that the coagulation properties were improved in more concentrated platelet products. The clotting time was increased by 7.0 s (p < 0.001), the CFT decreased by 20.5 s (p = 0.031), and the MCF was increased by 8.5 mm (p < 0.001) when less plasma was added (Figure 4). ...
Context 4
... showed that all three parameters were significantly affected by the added volume of plasma, indicating that the coagulation properties were improved in more concentrated platelet products. The clotting time was increased by 7.0 s (p < 0.001), the CFT decreased by 20.5 s (p = 0.031), and the MCF was increased by 8.5 mm (p < 0.001) when less plasma was added (Figure 4). ...
