Fig 1 - available via license: CC BY
Content may be subject to copyright.
H&E staining and Ki-67 expression by immunohistochemistry in primary cutaneous malignant melanoma (PCMM). The marker (brown) is expressed in the nuclei of PCMM cells. a: less than 1% of positivity and (b): corresponding to H&E staining; c greater than 60% of positivity and (d) corresponding to H&E staining
Source publication
The capacity for prognostic prediction of cutaneous melanoma, one of the most aggressive cancers, is still difficult due to the tumor heterogeneity and lack of reliable tumor markers. The objective of this study is to correlate, through immunohistochemistry, a Ki-67 and Kindlin-1 staining in malignant melanomas with the prognosis of the disease. A...
Context in source publication
Context 1
... scores of the two pathologists were compared, and any discrepancy was re-examined to achieve a consensus score. Figures 1 and 2 show some immunohistochemistry photomicrographs and their scores with each respective hematoxylin and eosinstained section (images captured by Invitrogen EVOS FL Auto 2.0 Imaging System). ...
Similar publications
Background: High levels of uric acid concentration over normal pregnant values have been reported previously in established preeclampsia, hence it was suggested to be a useful biochemical prognostic marker of the disease.
Methods: This is a hospital based prospective case control study where 100 patients with preeclampsia were recruited consecutiv...
Citations
... Ki67 is a cell cycle control protein whose specific antibody is used to ascertain the existence of a nuclear antigen only present in tissues with a high cellular proliferation rate, while it is otherwise absent in normal tissue. Ki67 is also involved in the transcription of RNA (23). It is absent during the G0 resting phase but present during the active cellular division phases G1, S, G2 and mitosis (24). ...
... Since the protein's discovery in 1983, Ki67 proved to be a reliable index in both the diagnosis and the prognosis of various types of cancer (23). Specifically, in melanoma, Ki67 is useful for the prevention of false-negative diagnoses of melanoma during the differential diagnosis from benign nevi (24). ...
Malignant melanoma is a melanocytic neoplasm with a steadily increasing incidence worldwide. In order to define a proper diagnostic protocol and to establish an accurate prognostic method for the disease, specific biomarkers are of notable importance. Their contribution is also significant in the treatment of melanoma for the improvement of newer and more targeted therapeutic approaches. To emphasize the importance of specific immune markers in the diagnosis of melanoma, immunohistochemical analysis was performed on 56 formalin-fixed paraffin-embedded cutaneous melanomas. Besides the traditional prognostic factors, depth of invasion and mitotic rate, the markers tested in the present study were S100 protein family, Melan A, Ki67 and HMB-45. The present results indicated that immunocytochemistry represents a valuable test in the diagnosis and treatment of malignant melanoma and each biomarker had different associations with the progression and prognosis of the disease. Patients with S100 expression were 4.83 times (95% CI=1.2-20.8) more likely to suffer a relapse, whereas patients with a Ki67 expression of >30% had a 5.41-fold higher risk (95% CI=1.3-22.0). The correlation between S100 and the Breslow depth was statistically significant (r-value: 0.43; P=0.027). In addition, the importance of a multidisciplinary team including a plastic surgeon, anatomopathologist and oncologist was highlighted.
