Growth and morphological features. a) Fungal colonies of 3 days' culture for the rst time, from the patient's BALF; b) growing in the LB broth; c) Colony morphology of M. cirrosus SZ 2021 cultured on chocolate medium for three days; d) Colony morphology of M. cirrosus SZ 2021 cultured on chocolate medium for a week; e) The conidia and septate hyphae under the microscope (1000x) by dyeing with Lactophenol cotton blue; f, g) Morphology of ascoma after lactophenol cotton blue staining under light microscopy (400x); h) Ascospores: pale yellow parts, crescent-shaped, not bluestained(1000x).

Contexts in source publication

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... in this BALF. As symptoms worsen, BALF from left upper bronchus on March 10, 2021 was secondly collected for mNGS. Nocardia nova (248 reads), Enterococcus (257reads), Aspergillus (60 reads), Parvovirus (71 reads) and Cytomegalovirus (20reads) were detected by mNGS in the BALF. Some white velvety colonies grew in the BALF after 3 days incubation (Fig. 3), with no evidence of mycobacterial, or additional fungal pathogens isolated. Because the accurate morphology of this fungus had not been established and no M. cirrosus were listed in MALDI-TOF-MS library, this fungus had never been correctly identied as a Microascus species until a month later. In a word, many results presented some ...

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... fungus was detected from the BALF of the patient by growing in both Columbia blood plate and chocolate agar plate for two days. The primary colonies were white and approximately 3-4 mm in diameter (Fig. 3a). Then it was incubated on many media to (Fig. 3c). Over 12 mm colonies with folds around the periphery and dark pigment in the center could be observed on culture medium for about a week (Fig. 3d). It could also grow at the bottom of a liquid medium. After about one week of cultivation on chocolate agar, the ball-shaped, smooth conidia ...

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... fungus was detected from the BALF of the patient by growing in both Columbia blood plate and chocolate agar plate for two days. The primary colonies were white and approximately 3-4 mm in diameter (Fig. 3a). Then it was incubated on many media to (Fig. 3c). Over 12 mm colonies with folds around the periphery and dark pigment in the center could be observed on culture medium for about a week (Fig. 3d). It could also grow at the bottom of a liquid medium. After about one week of cultivation on chocolate agar, the ball-shaped, smooth conidia and septate hyphae could be observed under the ...

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... by growing in both Columbia blood plate and chocolate agar plate for two days. The primary colonies were white and approximately 3-4 mm in diameter (Fig. 3a). Then it was incubated on many media to (Fig. 3c). Over 12 mm colonies with folds around the periphery and dark pigment in the center could be observed on culture medium for about a week (Fig. 3d). It could also grow at the bottom of a liquid medium. After about one week of cultivation on chocolate agar, the ball-shaped, smooth conidia and septate hyphae could be observed under the microscope by dyeing with Lactophenol cotton blue (Fig. 3e). For about four weeks, in the dark and brown part of the fungal colony, ascoma and ...

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... the periphery and dark pigment in the center could be observed on culture medium for about a week (Fig. 3d). It could also grow at the bottom of a liquid medium. After about one week of cultivation on chocolate agar, the ball-shaped, smooth conidia and septate hyphae could be observed under the microscope by dyeing with Lactophenol cotton blue (Fig. 3e). For about four weeks, in the dark and brown part of the fungal colony, ascoma and ascospores could be formed as the forms of sexual reproduction of the fungus. Microscopically, ascoma were large and spherical-shaped, containing ascospores which were half-moon shaped (Fig. 3f-3h). Based on the presence of branched conidiophores bearing ...

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... observed under the microscope by dyeing with Lactophenol cotton blue (Fig. 3e). For about four weeks, in the dark and brown part of the fungal colony, ascoma and ascospores could be formed as the forms of sexual reproduction of the fungus. Microscopically, ascoma were large and spherical-shaped, containing ascospores which were half-moon shaped (Fig. 3f-3h). Based on the presence of branched conidiophores bearing cylindrical annellides in brush-like groups and on the development of small black ascomata (cleistothecia) after four weeks, it was morphologically identied as Microascus sp. Compared with the morphological description of M. cirrosus from seven clinical cases (Table 1), the M. ...