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Glycated hemoglobin content (%A1c) in erythrocytes left untreated or incubated for 24 h with 100 mM D-Gal with or without pre-exposure to 10 μM Q, or 10 μM Q alone. ns, not statistically
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Aging is a multi-factorial process developing through a complex net of interactions between biological and cellular mechanisms and it involves oxidative stress (OS) as well as protein glycation. The aim of the present work was to verify the protective role of Quercetin (Q), a polyphe-nolic flavonoid compound, in a D-Galactose (D-Gal)-induced model...
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... not statistically significant versus control; **, p < 0.01 and ***, p < 0.001 versus control; °°° , p < 0.001 versus 100 mM D-Gal, one-way ANOVA followed by Bonferroni's multiple comparison post hoc test (n = 6). Figure 6 shows the glycated hemoglobin levels (%A1c) measured in the erythrocytes left untreated or treated with 10 μM Q for 1 h or 100 mM D-Gal for 24 h with or without pre-treatment with 10 μM Q. The %A1c levels measured following the exposure to D-Gal were significantly increased with respect to those of the control (untreated erythrocytes). ...
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... the glycated hemoglobin content (%A1c) was measured. The results confirmed that the D-Gal-treated erythrocytes (100 mM) showed an increase in glycated hemoglobin (Figure 6), which was fully prevented by pre-treatment with 10 μM Q. Thus, these results confirm that post-translational changes of intracellular proteins, including hemoglobin, might affect B3p function during aging and further support a significant anti-glycation effect of Q on hemoglobin. ...
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... not statistically significant versus control; **, p < 0.01 and ***, p < 0.001 versus control; °°° , p < 0.001 versus 100 mM D-Gal, one-way ANOVA followed by Bonferroni's multiple comparison post hoc test (n = 6). Figure 6 shows the glycated hemoglobin levels (%A1c) measured in the erythrocytes left untreated or treated with 10 μM Q for 1 h or 100 mM D-Gal for 24 h with or without pre-treatment with 10 μM Q. The %A1c levels measured following the exposure to D-Gal were significantly increased with respect to those of the control (untreated erythrocytes). ...
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... the glycated hemoglobin content (%A1c) was measured. The results confirmed that the D-Gal-treated erythrocytes (100 mM) showed an increase in glycated hemoglobin (Figure 6), which was fully prevented by pre-treatment with 10 μM Q. Thus, these results confirm that post-translational changes of intracellular proteins, including hemoglobin, might affect B3p function during aging and further support a significant anti-glycation effect of Q on hemoglobin. ...
Citations
... However, it is interesting to note that the improved myocardial CAT and SOD activity as well as GSH levels reported in GBS-treated rats suggest lower production of harmful intermediates and, as a result, cardioprotection. Remigante et al. (2022) and Asiwe et al. (2022a) point out that decreased GSH is an essential component of erythrocytes and plays a critical role in protecting against oxidative damage to the myocardium. Due to the formation of a disulfide (GSSG) when the thiol group of its cysteine residue is oxidized, its tripeptide can act as an antioxidant ( Pisoschi et al., 2021 ;Remigante et al., 2022 ;Isibor et al., 2022 ). ...
... Remigante et al. (2022) and Asiwe et al. (2022a) point out that decreased GSH is an essential component of erythrocytes and plays a critical role in protecting against oxidative damage to the myocardium. Due to the formation of a disulfide (GSSG) when the thiol group of its cysteine residue is oxidized, its tripeptide can act as an antioxidant ( Pisoschi et al., 2021 ;Remigante et al., 2022 ;Isibor et al., 2022 ). Also, in this study, animals exposed to Cs-A had reduced levels of GSH with increased MDA concentrations relative to normal controls. ...
... The presence of the ortho-phenolic hydroxyl group in the phenolic hydroxyl structure allows for easy oxidation, leading to the creation of a quinone structure. This structure demonstrates a notable ability to remove free radicals, particularly ROS [16,17]. Moreover, these polyphenols possess the capacity to activate cellular signaling pathways and trigger the production of antioxidant enzymes. ...
The biochemical characteristics of polyphenols contribute to their numerous advantageous impacts on human health. The existing research suggests that plant phenolics, whether consumed orally or applied directly to the skin, can be beneficial in alleviating symptoms and avoiding the development of many skin disorders. Phenolic compounds, which are both harmless and naturally present, exhibit significant potential in terms of counteracting the effects of skin damage, aging, diseases, wounds, and burns. Moreover, polyphenols play a preventive role and possess the ability to delay the progression of several skin disorders, ranging from small and discomforting to severe and potentially life-threatening ones. This article provides a concise overview of recent research on the potential therapeutic application of polyphenols for skin conditions. It specifically highlights studies that have investigated clinical trials and the use of polyphenol-based nanoformulations for the treatment of different skin ailments.
... However, the dose is selected based on available evidence, and we should have extensively checked the doses as this expanding field of research is now demanding[38]. Aging of gastrointestinal mucosa as well as the age-related cellular susceptibility to diabetesassociated oxidative stress[39][40] are also variables worth future investigation. All studied populations were receiving metformin. ...
diabetic effect of a combination of black seed (Nigella sativa) and Cumin (Cuminum cyminum), a two-step study from bench to bed. ABSTRACT Background: Type 2 Diabetes mellitus (T2DM) is a prevalent chronic condition causing one-fifth million deaths globally.
... [17][18][19] Recently, we have set up an oxidative stressrelated model of aging in human erythrocytes, which is characterized by elevated oxidative stress markers and functional alterations of the anion exchanger SLC4A1/AE1/ band3, the main ion transport system in erythrocytes. [20][21][22][23][24][25] Based on these findings, we hypothesized that other ion channels and transporters can be targets of oxidative stress during aging. As alterations in brain function are a major hallmark of aging, and neuroglia, particularly astrocytes, are fundamental in preserving brain physiology, we established a new model of aging neuroglia in U-87 MG glioma cells and studied the ion transport in this setting. ...
Melatonin is a pleiotropic biofactor and an effective antioxidant and free radical scavenger and, as such, can be protective in oxidative stress‐related brain conditions including epilepsy and aging. To test the potential protective effect of melatonin on brain homeostasis and identify the corresponding molecular targets, we established a new model of oxidative stress‐related aging neuroglia represented by U‐87 MG cells exposed to D‐galactose (D‐Gal). This model was characterized by a substantial elevation of markers of oxidative stress, lipid peroxidation, and protein oxidation. The function of the inward rectifying K ⁺ channel Kir2.1, which was identified as the main Kir channel endogenously expressed in these cells, was dramatically impaired. Kir2.1 was unlikely a direct target of oxidative stress, but the loss of function resulted from a reduction of protein abundance, with no alterations in transcript levels and trafficking to the cell surface. Importantly, melatonin reverted these changes. All findings, including the melatonin antioxidant effect, were reproduced in heterologous expression systems. We conclude that the glial Kir2.1 can be a target of oxidative stress and further suggest that inhibition of its function might alter the extracellular K ⁺ buffering in the brain, therefore contributing to neuronal hyperexcitability and epileptogenesis during aging. Melatonin can play a protective role in this context.
... It is even known that quercetin plays a role as a healing agent in neurodegenerative pathologies since it easily crosses the blood-brain barrier due to its lipophilic properties [47,48]. On the other hand, it is known that quercetin has a protective effect on human erythrocytes in a model of aging induced by D-Galactose (D-Gal), effectively preventing oxidative damage to membrane lipids through the B3 protein [49]. ...
... The erythroprotective potential of biomolecules such as proteins, phycobiliproteins and carotenoids is associated with the presence of blood groups. A study by Remigante et al. [49] verified the protective effect of quercetin on human erythrocytes in an aging model induced by D-Galactose (D-Gal). They observed that quercetin at a concentration of 10 µM has a protective effect on the function of the B3 protein (B3P). ...
... The evaluation of B3P function could be a useful tool for monitoring erythrocyte homeostasis; at the same time, B3P is identified as a potential target of antioxidant treatments to counteract uncontrolled oxidative discharges related to aging. Despite this, it is necessary to delve deeper into the erythroprotective action of QUE to determine the effectiveness of using this flavonoid in the pharmacological industry against oxidative stress [47,49]. ...
Previous studies detail that different blood groups are associated with incidence of oxidative stress-related diseases such as certain carcinomas. Bioactive compounds represent an alternative for preventing this oxidative stress. The aim of this study was to elucidate the impact of blood groups on the erythroprotective potential of fucoxanthin, β-Carotene, gallic acid, quercetin and ascorbic acid as therapeutic agents against oxidative stress. The impact of ABO blood groups on the erythroprotective potential was evaluated via the antioxidant capacity, blood biocompatibility, blood susceptibility and erythroprotective potential (membrane stabilization, in vitro photostability and antihemolytic activity). All tested antioxidants exhibited a high antioxidant capacity and presented the ability to inhibit ROO•-induced oxidative stress without compromising the cell membrane, providing erythroprotective effects dependent on the blood group, effects that increased in the presence of antigen A. These results are very important, since it has been documented that antigen A is associated with breast and skin cancer. These results revealed a probable relationship between different erythrocyte antigens with erythroprotective potential, highlighting the importance of bio-targeted drugs for groups most susceptible to certain chronic-degenerative pathologies. These compounds could be applied as additive, nutraceutical or encapsulated to improve their bioaccessibility.
... Oxidative products are highly reactive, and can directly or indirectly modulate the functions of many enzymes and transcription factors through complex signaling cascades. In particular, some of the pathways are preferentially linked to enhanced survival, while others are more frequently associated with cell death, and constitute important avenues for therapeutic interventions aimed at limiting oxidative damage or, alternatively, attenuating its consequences [5][6][7][8][9][10][11]. Furthermore, the magnitude and exposure of the insult, as well as the cell type involved, are key elements in defining which pathways are activated, as well as the final cell outcome. ...
Oxidative stress is frequently described as the balance between the production of reactive species (including oxygen and nitrogen) in biological systems and the ability of the latter to defend itself through the sophisticated antioxidant machinery [...]
... Such evidences contribute to evaluating the mechanisms underlying aging process in human erythrocytes and suggest that foods containing flavonoids may be useful in treatment and prevention of oxidative stress-related alterations. In this context, it has been already reported that a dietary intake of vegetables and fruits may be effective in increasing antioxidant capacity at plasma level [45,47,49,50] and, specifically, the fruit of Açaí palm is reported to display an antioxidant capability. ...
... Using Dixon calculations, the outliers' results were removed (a total of eight measurements) [43]. The statistical hypothesis that the impact of carbon monoxide poisoning severity on antioxidative parameters is significant was verified using an analysis of variance (ANOVA) [44][45][46][47][48][49][50][51]. Preliminary analysis (two-factor ANOVA) showed no statistically significant differences in dependent variables between men and women. ...
In conventional clinical toxicology practice, the blood level of carboxyhemoglobin is a biomarker of carbon monoxide (CO) poisoning but does not correspond to the complete clinical picture and the severity of the poisoning. Taking into account articles suggesting the relationship between oxidative stress parameters and CO poisoning, it seems reasonable to consider this topic more broadly, including experimental biochemical data (oxidative stress parameters) and patients poisoned with CO. This article aimed to critically assess oxidative-stress-related parameters as potential biomarkers to evaluate the severity of CO poisoning and their possible role in the decision to treat. The critically set parameters were antioxidative, including catalase, 2,2-diphenyl-1-picryl-hydrazyl, glutathione, thiol and carbonyl groups. Our preliminary studies involved patients (n = 82) admitted to the Toxicology Clinical Department of the University Hospital of Jagiellonian University Medical College (Kraków, Poland) during 2015–2020. The poisoning was diagnosed based on medical history, clinical symptoms, and carboxyhemoglobin blood level. Blood samples for carboxyhemoglobin and antioxidative parameters were collected immediately after admission to the emergency department. To evaluate the severity of the poisoning, the Pach scale was applied. The final analysis included a significant decrease in catalase activity and a reduction in glutathione level in all poisoned patients based on the severity of the Pach scale: I°–III° compared to the control group. It follows from the experimental data that the poisoned patients had a significant increase in level due to thiol groups and the 2,2-diphenyl-1-picryl-hydrazyl radical, with no significant differences according to the severity of poisoning. The catalase-to-glutathione and thiol-to-glutathione ratios showed the most important differences between the poisoned patients and the control group, with a significant increase in the poisoned group. The ratios did not differentiate the severity of the poisoning. The carbonyl level was highest in the control group compared to the poisoned group but was not statistically significant. Our critical assessment shows that using oxidative-stress-related parameters to evaluate the severity of CO poisoning, the outcome, and treatment options is challenging.
... Quercetin is one of the most abundant flavonoids in the daily diet and has been studied as an anti-cardiovascular, anti-cancer, anti-viral, and antioxidant drug (92)(93)(94)(95)(96). In recent years, studies have found that quercetin has the potential to treat HUA, and as a result, more and more research has been conducted on quercetin for the treatment of HUA (97). ...
Hyperuricemia is another common metabolic disease, which is considered to be closely related to the development of many chronic diseases, in addition to the “three highs.” Currently, although drugs show positive therapeutic effects, they have been shown to produce side effects that can damage the body. There is growing evidence that medicinal and edible plants and their bioactive components have a significant effect on hyperuricemia. In this paper, we review common medicinal and edible plants with uric acid-lowering effects and summarize the uric acid-lowering mechanisms of different bioactive components. Specifically, the bioactive components are divided into five categories: flavonoids, phenolic acids, alkaloids, polysaccharides, and saponins. These active substances exhibit positive uric acid-lowering effects by inhibiting uric acid production, promoting uric acid excretion, and improving inflammation. Overall, this review examines the potential role of medicinal and edible plants and their bioactive components as a means of combating hyperuricemia, with the hope of providing some reference value for the treatment of hyperuricemia.
... 9 Antioxidant intervention has proven to reduce the effect of aging on erythrocytes. 10 Antioxidants as additives such as Trolox, curcumin, carnosine, spermine, phloretin and ascorbic acid have proven to attenuate the oxidative insult during erythrocyte storage. [11][12][13][14] Studies have reported the preventive effects of p-coumaric acid (3-(4-hydroxyphenyl)-2-propenoic acid; CA), a phenolic acid which is found widely distributed in plants and as a part of human diet. ...
Introduction and aim. Stored erythrocytes develop lesions involving changes in their structure and function reducing their efficacy. Oxidative Stress (OS) being one of the main causes of storage lesion, can be attenuated by antioxidants as additives in the storage solution. This study aims to evaluate the effect of p-Coumaric acid (CA) on erythrocytes during whole blood storage. Material and methods. Blood collected from Male Wistar rats was stored at 4°C in CPDA-1 solution for 21 days. Blood samples were stored with and without 1mM CA (CA 1) and 10 mM CA (CA 10). The erythrocytes were isolated every week during storage and the biomarkers for OS and antioxidant status were analysed. Results. Superoxide dismutase and catalase elevated on day 14. Conjugate dienes decreased in CA 10 on day 14. Thiobarbituric acid reactive substances increased on day 7 and decreased on day 14 in CA groups. Protein sulfhydryls decreased in controls and CA 1 on day 14 whereas, it was maintained in CA 10. Conclusion. Coumaric acid upregulated the antioxidant enzymes and protected the cells from oxidative damage. Thus, coumaric acid can be employed as a potent additive during storage and opens new avenues of employing it in similar OS situations in erythrocytes.
