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Characterization of microbial communities is important for understanding the soil biodiversity distribution affected by environmental factors. Here, we combined high-throughput sequencing of 16S rDNA and ITS to investigate the composition of bacterial and fungal communities in interval seawater covering Linchang Island, China. We compared the micro...
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The present study aims to carefully delineate the bacterial community composition in marine sediments from different geographical coastal regions of Palk Bay and Gulf of Mannar that are known for human recreational activities. Bacterial richness in different marine sediments was assessed using 16S rRNA gene-based Denaturing Gradient Gel Electrophor...
Citations
... NanoDrop ND-1000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, United States) and agarose gel electrophoresis were used to measure the quantity and quality of DNA. Bacterial and fungal abundance was determined by real-time quantitative polymerase chain reaction (Chen et al., 2021). The primers 338F (5'-ACTCCTACGGGAGGCAGCA-3') and 519R (5'-ATTACCGCGGCTGCTGG-3') (Zhang et al., 2017) and the primers ITS5 (5'-GGAAGTAAAAGTCGTAACAAGG-3') and ITS2 (5'-GCTGCGTTCTTCATCGATGC-3') (White et al., 1990) were used to evaluate the 16S rRNA and ITS gene copy number, respectively. ...
... Primers ITS5 (5'-GGAAGTAAAAGTCGTAACAAGG-3') and ITS2 (5'-GCTGCGTTCTTCATCGATGC-3') were used to amplify the ITS1 region (White et al., 1990). PCR amplification and product purification were carried out as described by Chen et al. (2021). Sequencing was performed using the Illumina MiSeq platform at Shanghai Personal Biotechnology Co., Ltd. ...
Coastal lagoon is an important productive ecosystem on the Earth. In this study, we compared microbial community in the sediments between lagoon and surrounding areas, and explored mechanism for the variation of microbial community. As a result, the sediment of surrounding area showed significantly higher organic matter and total nitrogen than that of the lagoon. The linear regression analysis revealed that organic matter and total nitrogen are positively correlated with Xenococcus. Bacterial and fungal PCoA1 showed significantly positive relationships with the relative abundance of Xenococcus, indicating that Xenococcus affects the bacterial and fungal community in the sediments of both the lagoon and surrounding area. ANOSIM analysis demonstrated that there were significant differences in bacterial and fungal community structure in the sediments between the lagoon and surrounding areas. Therefore, organic matter and total nitrogen affect the microbial community structure in the sediments of lagoon and surrounding areas by regulating the abundance of Xenococcus.
