Genome Assembly Statistics for Ramalina Farinacea (new to This Study) Compared With Published Ramalinaceae Genome Assemblies

Genome Assembly Statistics for Ramalina Farinacea (new to This Study) Compared With Published Ramalinaceae Genome Assemblies

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Lichen-forming fungi are a diverse and ecologically important group of obligate mutualistic symbionts. Due to difficulties with maintaining them in culture and their extremely slow growth, lichenologists are increasingly opting for metagenomic sequencing followed by symbiont genome separation using bioinformatic pipelines. However, without knowing...

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Context 1
... is comparable with the available long-read assemblies for the Lecanoromycetes genera Bacidia, Physcia, Letharia, and Umbilicaria (McKenzie et al. 2020;Tzovaras et al. 2020;Wilken et al. 2020;Allen et al. 2021;Singh et al. 2021;Gerasimova et al. 2022). The assembly had a BUSCO completeness score of 95.8% (table 1). ...
Context 2
... annotation predicted 8,765 proteins, and secondary metabolite biosynthetic gene cluster (BGC) analyses identified 52 BGCs; 25 polyketide synthases (PKSs), 16 nonribosomal peptide synthetases (NRPSs), 4 terpenes, 5 NRPS-PKS hybrids, and 1 indole cluster. Ramalina farinacea has a similar number of BGCs to Ramalina intermedia and Ramalina peruviana which have 54 and 43 clusters, respectively (table 1). The number of BGCs is significantly higher than in the two published Bacidia genomes, supporting a previous observation by Gerasimova et al. (2022). ...

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