Fig 7 - uploaded by Eugen Carasevici
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Gel migration of DNA extracted 72 hours after incubation of irradiated Jurkat cells in fresh RPMI1640 culture medium or incubation in the same culture medium. Lines 2, 3: DNA extracted from Jurkat, irradiated with 0.5 Gy and 2.5 Gy incubated in fresh RPMI 1640. Line 4: DNA extracted from Jurkat, irradiated with 2.5 Gy and lines 1 and 5: 50 bp ladder marker.
Source publication
The present study was designed to evaluate the radio-induced effects in culture medium with or without cells. Two leukemia cell lines (Jurkat and K562) were used in this study. The culture medium and cell cultures were irradiated with 0 Gy, 0.5 Gy, and 2.5 Gy. We investigated the radio-induced effects for unirradiated cells incubated in separately...
Citations
... After irradiation of the PB-MNCS the cells were not washed, because it has been shown previously with other cell types that there is no difference in the rate of apoptosis if the cells are kept in the original irradiated medium or switched to fresh medium [44]. Also, an additional wash (centrifugation) step has the potential to produce additional damage to cells [45], which would complicate the interpretation of the effects of radiation alone on the invasion assay results. ...
Regenerative medicine studies using autologous bone marrow mononuclear cells (BM-MNCs) have shown improved clinical outcomes that correlate to in vitro BM-MNC invasive capacity. The current Boyden-chamber assay for testing invasive capacity is labor-intensive, provides only a single time point, and takes 36 hours to collect data and results, which is not practical from a clinical cell delivery perspective. To develop a rapid, sensitive and reproducible invasion assay, we employed Electric Cell-substrate Impedance Sensing (ECIS) technology. Chemokine-directed BM-MNC cell invasion across a Matrigel-coated Transwell filter was measurable within minutes using the ECIS system we developed. This ECIS-Transwell chamber system provides a rapid and sensitive test of stem and progenitor cell invasive capacity for evaluation of stem cell functionality to provide timely clinical data for selection of patients likely to realize clinical benefit in regenerative medicine treatments. This device could also supply robust unambiguous, reproducible and cost effective data as a potency assay for cell product release and regulatory strategies.
This study evaluated the cytotoxic effect produced by Magainin II, Cecropin A and ecropin B on four tumor cell lines. The cytotoxic effect of the Magainin II, Cecropin A and Cecropin B by the MTT [3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide] viability test was evaluated. Peptides used did not show the same cytotoxic effect on all cell lines tested; which means that the cytotoxic effect of tested peptides depends on the tumor cell used in this experiment. Cytotoxic effect was more pronounced with increasing concentration of peptide. The antimicrobial peptides used in our experiment had variable potential on A549 human alveolar carcinoma cells.
