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Galiellalactone has no effect on blood cell counts in western type diet-treated ApoE-deficient mice. ApoE-deficient mice were either fed for 18 weeks with normal chow diet (ND) or western-type diet (WD) (treated with Galiellalactone (Gal) or vehicle (PBS/EtOH) for 6 weeks). Citrated whole mouse blood was collected by intra-cardial puncture. Platelet counts (A), white blood cell counts (WBCs; B) as well as red blood cell counts (RBCs; C) were determined using an automatic cell counter. Data shown are mean + SEM of 4–6 mice (ns = not significant vs. WD + PBS/EtOH; one-way ANOVA). doi:10.1371/journal.pone.0130401.g004  

Galiellalactone has no effect on blood cell counts in western type diet-treated ApoE-deficient mice. ApoE-deficient mice were either fed for 18 weeks with normal chow diet (ND) or western-type diet (WD) (treated with Galiellalactone (Gal) or vehicle (PBS/EtOH) for 6 weeks). Citrated whole mouse blood was collected by intra-cardial puncture. Platelet counts (A), white blood cell counts (WBCs; B) as well as red blood cell counts (RBCs; C) were determined using an automatic cell counter. Data shown are mean + SEM of 4–6 mice (ns = not significant vs. WD + PBS/EtOH; one-way ANOVA). doi:10.1371/journal.pone.0130401.g004  

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... analyze whether Gal changes the numbers of the different blood cells, we performed blood cell counts. As seen in Fig 4, no effects of Gal on the blood cells counts in WD-fed ani- mals were detected. Galiellalactone reduces the expression of pro-inflammatory marker genes in pro-atherosclerotic mice. ...