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GUS histochemical staining of different tissues in transgenic tobacco with NtR12::GUS. A Semi-quantitative GUS reaction activities of root, leaf, and stem in NtR12::GUS transformed tobacco. Tubes NtR-R, NtR-S, NtR-L represent roots, stem, and leaves of NtR12::GUS transgenic plants, respectively; Tube NtR-L, no staining visible in leaves; Tubes 35S-R, 35S-S, 35S-L show GUS staining of root, leaf, and stem in 35S::GUS transgenic tobacco, respectively. GUS staining was found to be visible in all tubes. Root GUS activity was driven to a higher extent by NtR12 promoter than by the CaMV35S promoter. B This figure show GUS staining patterns in transgenic plant tissues with NtR12 or CaMV35S promoters, respectively. GUS expressed more evenly in NtR12::GUS transgenic root; GUS activity lower in stem cells in NtR12::GUS transgenic lines, but no GUS activity was visible in the leaf of transgenic plants. C and D GUS staining present in parenchyma cells and also root hairs in vascular bundles of root. Scale bars indicate 100.00 μm in C, 20.00 μm in D
Source publication
Background
Tobacco is an important economic crop, but the quality and yield have been severely impaired by bacterial wilt disease (BWD) caused by Ralstonia solanacearum.
Methods and results
Here, we describe a transgenic approach to prevent BWD in tobacco plants. A new root-specific promoter of an NtR12 gene was successfully cloned. The NtR12 prom...
