GFP expression of construct 3 in yolk sac and vitelline vessels (E11.5). (AA’- DD’) Yolk sac; (EE’) Vitelline vessels. Note that qualitatively similar results are obtained with construct 2 (for example see C), but no expression is seen with construct 1, and no non-specific fluorescence is detected with non-transgenic embryos. 

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... constructs 2 and 3, fluorescent cells are visible extraembryonically in the yolk sac starting at E8-8.5 through E11.5-12, and in vitelline vessels (Fig. 5). In the yolk sac (Fig. 5AA’-DD’), many positive cells are spread in the mesenchyme, but often tend to cluster within or along small vessels. In vitelline vessels (Fig. 5EE’), cells are within the wall or in the blood stream. In the fetal liver, fluorescence appears between E10-10.5 persisting up to E12.5-13 (see below). Postnatally, the bone marrow is strongly fluorescent (not shown). Importantly, no expression is detected with construct ...