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Fungal cultures and microscopic appearances of species used as rearing mediums of Aphelenchoides besseyi; Alternaria padwickii (A, D), Fusarium semitectum (B, E), Botrytis cinerea (C, F)
Source publication
White tip disease on rice caused by Aphelenchoides besseyi is categorized as emerging infectious disease in Indonesia because the disease incidence and its geographical distributionare increasing rapidly. Until presently, there is no information related with mass culture technique of Ap. besseyi in Indonesia. Thus, studies to obtain pure population...
Context in source publication
Context 1
... of B. cinerea on PDA medium were greyish brown with white cotton-like mycelium on the edges. Conidiophores were long, slim, contained hyaline, irregularly branched on top, and apical cells were larger and round (Figure 1). Rearing of Ap. besseyi resulted in various growth stages of nematodes, including juvenile to mature nematodes ( Figure 2). ...
Citations
Once a nematode has been identified, to conduct studies for screening programs or pathogenicity tests, it is necessary a supply of large numbers of nematodes from field crops or reproduced and stored to be used in periods of the year when they are not available from fields. Therefore, nematodes must be reared in greenhouse or under in vitro conditions and stored for future needs. In this chapter, suggestions are given on how to obtain nematodes from fields and reproduce most of them on host plants in greenhouse (mainly Meloidogyne spp. and Globodera spp.) or in vitro. Reproductions in vitro include:
On suitable callus of host plants (Pratylenchus spp., Ditylenchus spp.)
On fungal cultures mainly of Botrytis cinerea or Alternaria spp. for Aphelenchoides spp. and other aphelenchids, including Bursaphelenchus xylophilus.
On carrot disks for Pratylenchus spp. and Ditylenchus spp.
Other specific media, such as garlic, potato, and sweet potato for D. destructor, and cocoyam disks for Radopholus similis.
Guidelines are also given to store different nematodes for rather long times, including in vitro methods and in infected seeds, hay, and other plant parts. No information is given on how to prepare and store fixed materials.
