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Schematic diagram of the regulation of sugar beet taproot growth and development. Taproot growth and expansion might be regulated at RNA metabolism, protein metabolism, and cell wall metabolism. RNA metabolism relative genes promote RNA catabolism, RNA helicase, and RNA glycosidic bond hydrolysis to restrict the formation of proteins in many processes. Protein metabolism relative genes involved in peptide bonds hydrolysis, BAG6 protein catabolism, and protein spatial structure refolding and maintaining protein integrity to assist in the correct posttranslational assembly of proteins. Cell wall metabolism relative genes involved in pectin, chitin, cellulose, lichenin, D-glucans, xyloglucan hydrolysis, and reconnection, promote the loosening and elongation of the cell wall, and improve cell elongation or expansion. Brassinolide and gibberellin receptor genes are enriched in pathway of plant hormone signal transduction. The red font indicates upregulated, and the green font represents downregulated in both transcriptome and proteome; and the blue font represents downregulated in transcriptome and upregulated in proteome.

Schematic diagram of the regulation of sugar beet taproot growth and development. Taproot growth and expansion might be regulated at RNA metabolism, protein metabolism, and cell wall metabolism. RNA metabolism relative genes promote RNA catabolism, RNA helicase, and RNA glycosidic bond hydrolysis to restrict the formation of proteins in many processes. Protein metabolism relative genes involved in peptide bonds hydrolysis, BAG6 protein catabolism, and protein spatial structure refolding and maintaining protein integrity to assist in the correct posttranslational assembly of proteins. Cell wall metabolism relative genes involved in pectin, chitin, cellulose, lichenin, D-glucans, xyloglucan hydrolysis, and reconnection, promote the loosening and elongation of the cell wall, and improve cell elongation or expansion. Brassinolide and gibberellin receptor genes are enriched in pathway of plant hormone signal transduction. The red font indicates upregulated, and the green font represents downregulated in both transcriptome and proteome; and the blue font represents downregulated in transcriptome and upregulated in proteome.

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Sugar beet taproot growth and development is a complex biological process involving morphogenesis and dry matter accumulation. However, the molecular regulatory mechanisms underlying taproot growth and development remain elusive. We performed a correlation analysis of the proteome and transcriptome in two cultivars (SD13829 and BS02) at the start a...

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... Starch and sucrose metabolic pathways are altered during turnip taproot development [16]. In addition, reduced lignification and altered cell wall metabolism have been suggested to contribute to the loosening and elongation of the cell wall, thus promoting the enlargement of taproots [14,17]. ...
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Background Stephania kwangsiensis Lo (Menispermaceae) is a well-known Chinese herbal medicine, and its bulbous stems are used medicinally. The storage stem of S. kwangsiensis originated from the hypocotyls. To date, there are no reports on the growth and development of S. kwangsiensis storage stems. Results The bulbous stem of S. kwangsiensis, the starch diameter was larger at the stable expanding stage (S3T) than at the unexpanded stage (S1T) or the rapidly expanding stage (S2T) at the three different time points. We used ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) and Illumina sequencing to identify key genes involved in bulbous stem development. A large number of differentially accumulated metabolites (DAMs) and differentially expressed genes (DEGs) were identified. Based on the differential expression profiles of the metabolites, alkaloids, lipids, and phenolic acids were the top three differentially expressed classes. Compared with S2T, significant changes in plant signal transduction and isoquinoline alkaloid biosynthesis pathways occurred at both the transcriptional and metabolic levels in S1T. In S2T compared with S3T, several metabolites involved in tyrosine metabolism were decreased. Temporal analysis of S1T to S3T indicated the downregulation of phenylpropanoid biosynthesis, including lignin biosynthesis. The annotation of key pathways showed an up-down trend for genes and metabolites involved in isoquinoline alkaloid biosynthesis, whereas phenylpropanoid biosynthesis was not completely consistent. Conclusions Downregulation of the phenylpropanoid biosynthesis pathway may be the result of carbon flow into alkaloid synthesis and storage of lipids and starch during the development of S. kwangsiensis bulbous stems. A decrease in the number of metabolites involved in tyrosine metabolism may also lead to a decrease in the upstream substrates of phenylpropane biosynthesis. Downregulation of lignin synthesis during phenylpropanoid biosynthesis may loosen restrictions on bulbous stem expansion. This study provides the first comprehensive analysis of the metabolome and transcriptome profiles of S. kwangsiensis bulbous stems. These data provide guidance for the cultivation, breeding, and harvesting of S. kwangsiensis.