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A new bidesmosidic triterpene saponin, guaianin P was isolated from the stem bark of Guaiacum officinale. Its structure was established as oleanolic acid 3-O-{α-L-rhamnopyranosyl-(1→3)-α-L-rhamnopyranosyl-(1→2)-[β-D-glucopyranosyl-(1→3)]-α-L-arabinopyranoside}-28-O-β-D-glucopyranosyl ester by spectroscopic and chem. analyses. [on SciFinder(R)]
Citations
... According to the World Health Organization (WHO), nearly 50,000 clinically important plants are being used in the pharmaceutical industry as traditional medicines (Msomi, N. Z., 2018). Guaiacum officinale, one of the traditional medicinal plants is effective against fish poisoning, HIV, as an abortifacient, in angina, tonsillitis, rheumatoid arthritis, mucosal diseases and metabolic abnormalities since ancient times (Ahmad, Bano, & Bano, 1984;Saba, Khatoon, Ali, & Ahmad, 2012). It is a perennial tree, slow-growing and predominantly existing in the West Indies, but are also distributed in different countries such as USA, Honduras, Panama, India and Pakistan (Cooper, 1986;Grandtner, 2005). ...
The present work is an attempt to find out biological potential of various extracts and ethanol fractions of Guaiacum officinale L. Sequential extraction was performed with different solvents. One of the active extracts (ethanol) was fractionated by column chromatography and the other (n-hexane) was profiled by GC-MS. The antimicrobial potential was tested against pathogenic bacteria by a well diffusion process and only one strain (S. aureus) displayed a significant resistance against different samples. For the cytotoxic effect, MTT assay was used on normal (BHK) and cancerous (HepG2) cell lines. n-Hexane, chloroform and ethanol extracts showed IC50 at 3.38, 3.56 and 2.607 mg/ml respectively. Furthermore, two fractions (i.e. F=2 and 3) fractions showed significant IC50 at 74.9 and 130.8 μg/ml respectively compared to the standard (Cisplatin, IC50 =16 μg/ml). No toxic effect was experienced against the normal cell line, showing it is safe in cancer therapy. The DPPH analysis revealed a significant antioxidant potential of the plant like n-hexane, chloroform and ethanol represented 68.67%, 73.00% and 77.67% RSA respectively, whereas F=1 to F=7 along with ascorbic acid showed moderate to less antioxidant effect, i.e. 55.93%, 68.56%, 50.19%, 9.64%, 28.40%, 12.63%, 32.54%, and 81.53%, correspondingly. Based on the above findings, it is highly recommended for further purification, isolation and identification of biomolecules from fruits of G. officinale as a possible extension of the present project.
... According to the World Health Organization (WHO), nearly 50,000 clinically important plants are being used in the pharmaceutical industry as traditional medicines (Msomi, N. Z., 2018). Guaiacum officinale, one of the traditional medicinal plants is effective against fish poisoning, HIV, as an abortifacient, in angina, tonsillitis, rheumatoid arthritis, mucosal diseases and metabolic abnormalities since ancient times (Ahmad, Bano, & Bano, 1984;Saba, Khatoon, Ali, & Ahmad, 2012). It is a perennial tree, slow-growing and predominantly existing in the West Indies, but are also distributed in different countries such as USA, Honduras, Panama, India and Pakistan (Cooper, 1986;Grandtner, 2005). ...
The present work is an attempt to find out biological potential of various extracts and ethanol fractions of Guaiacum officinale L. Sequential extraction was performed with different solvents. One of the active extracts (ethanol) was fractionated by column chromatography and the other (n-hexane) was profiled by GC-MS. The antimicrobial potential was tested against pathogenic bacteria by a well diffusion process and only one strain (S. aureus) displayed a significant resistance against different samples. For the cytotoxic effect, MTT assay was used on normal (BHK) and cancerous (HepG2) cell lines. n-Hexane, chloroform and ethanol extracts showed IC50 at 3.38, 3.56 and 2.607 mg/ml respectively. Furthermore, two fractions (i.e. F=2 and 3) fractions showed significant IC50 at 74.9 and 130.8 µg/ml respectively compared to the standard (Cisplatin, IC50 =16 µg/ml). No toxic effect was experienced against the normal cell line, showing it is safe in cancer therapy. The DPPH analysis revealed a significant antioxidant potential of the plant like n-hexane, chloroform and ethanol represented 68.67%, 73.00% and 77.67% RSA respectively, whereas F=1 to F=7 along with ascorbic acid showed moderate to less antioxidant effect, i.e. 55.93%, 68.56%, 50.19%, 9.64%, 28.40%, 12.63%, 32.54%, and 81.53%, correspondingly. Based on the above findings, it is highly recommended for further purification, isolation and identification of biomolecules from fruits of G. officinale as a possible extension of the present project.
... Elsewhere, there is abundant anecdotal evidence that the resins and extracts of G. officinale have antiinflammatory properties with activity against arthritis, gout and sciatica condition [8]. Furthermore, the resins of the plant are used in traditional medicines in Pakistan to cure angina, tonsillitis, rheumatoid arthritis, mucous membrane diseases and abnormalities of metabolic processes [9]. ...
... G. officinale, known to be a source of pure lignum vitae, is commonly found in tropical areas, mainly in Central, North and South America and including the Caribbean islands [24]; additionally, it has been introduced and cultivated in Karachi, Pakistan [30]. G. officinale is a slow-growing, small to medium sized tree that reaches 3e10 m in height [24]. ...
... Its extract has historically been used as a herbal medicine to cure some diseases [24]. The bark of G. officinale plants cultivated on the premises of the University of Karachi was collected, airdried, chopped and extracted with methanol, which was then concentrated under reduced pressure, to extract its medicinal bioactive compounds [30]. For the present study, the resulting treated and shade dried bark was used as a precursor to produce supercapacitor electrodes. ...
Binderless monoliths of supercapacitor electrodes were prepared by the
carbonization (N2) and activation (CO2) of green
monoliths (GMs). GMs were made from mixtures of self-adhesive carbon
grains (SACG) of fibers from oil palm empty fruit bunches and a
combination of 5 & 6% KOH and 0, 5 & 6% carbon nanotubes (CNTs)
by weight. The electrodes from GMs containing CNTs were found to have
lower specific BET surface area (SBET). The electrochemical
behavior of the supercapacitor fabricated using the prepared electrodes
were investigated by electrochemical impedance spectroscopy (EIS) and
galvanostatic charge-discharge (GCD). In general an addition of CNTs
into the GMs reduces the equivalent series resistance (ESR) value of the
cells. A cell fabricated using electrodes from GM with 5% CNT and 5% KOH
was found to have the largest reduction of ESR value than that from the
others GMs containing CNT. The cell has steeper Warburg's slope than
that from its respective non-CNT GM, which reflect the smaller
resistance for electrolyte ions to move into pores of electrodes despite
these electrodes having largest reduction in specific BET surface area.
The cell also has the smallest reduction of specific capacitance
(Csp) and maintains the specific power range despite a
reduction in the specific energy range due to the CNT addition.
Backgound: Guaiacum officinale L. is an alien species to Thailand. It is used as anti-Arthritis and anti-rheumatoid agents in Indian folklore medicine. Objective: The present study was aimed to investigate total phenolic contents and free radical scavenging activity of the extracts from Guaiacum officinale L. (Zygophyllaceae). Methods: The plant parts including bark, twig and leaf were extracted using different solvents (ethyl acetate, water and ethanol). Total phenolic contents were determined by Folin-Ciocalteu Colorimetry method while free radical scavenging activity of the extracts was investigated by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity assay. Results: The results show that the highest total phenolic content is found in the ethyl acetate twig extracts (20.3±0.0031 μg GAE/1 μg extracts). The ethanolic twig extracts had the highest free radical scavenging activity with IC50 of 0.45±0.0188 mg/ml. Conclusion: The extracts from Guaiacum officinale L. exhibit good anti-oxidant activity and may be suitable for development as drugs and supplementary food.
Binderless composite monolith (BCM) electrodes prepared from carbon nanotubes (CNTs) and self-adhesive carbon grains (SACGs) were used in a symmetrical supercapacitor. The SACGs were prepared from fibers of oil palm empty fruit bunches (EFBs) from oil palm tree (Elaeis guineensis), Heliotropium dasycarpum (H. dasycarpum) and Guaiacum officinale (G. officinale). For each biomass, the BCMs were prepared by the carbonization and activation of green monoliths (GMs) containing SACGs treated with KOH and a mixture of SACGs and CNTs treated with KOH. Thermal decomposition behavior of all SACGs was found to be slightly different because of the difference in their compositions. In addition, BCMs from H. dasycarpum and G. officinale were found to have SiO2. The BET surface areas were 1656, 1031 and 532 m2 g−1 for the BCMs from EFB, H. dasycarpum and G. officinale, respectively, and these values decreased by 40, 50 and 31% upon CNTs addition. Consequently, the specific capacitance decreased from ∼124 to ∼104 and ∼49 F g−1 to ∼111, ∼87 and ∼31 F g−1, respectively. However, addition of CNTs reduced the equivalent series resistance (ESR) by a factor of 83.9 (EFB), 90.6 (H. dasycarpum) and 38.8 (G. officinale) %. It was also found that CNTs addition contributed to improving the decay of Csp with increasing scan rate if the electrode surface area was sufficiently high.
