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Folic acid improved spatial learning and memory ability in adolescent and adult offspring. Dams and pups were fed as described in Figure 1. (A) Escape latency during the spatial acquisition
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Early life stage folate status may influence neurodevelopment in offspring. The developmental origin of health and disease highlights the importance of the period of the first 1000 days (from conception to 2 years) of life. This study aimed to evaluate the effect of early life stage folic acid deficiency on de novo telomere synthesis, neurobehavior...
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Context 1
... the phase of the acquisition test, both offspring PND50 and PND90 showed that the escape latency gradually shortened with the increase of experimental days. Through repeated-measures ANOVA, we found that in the offspring PND50 and PND90, the FA-D group had the most prolonged escape latency compared with the FA-N and FA-S groups (p < 0.05, Figure 3A,D); compared with the FA-S group, the FA-N group took longer time to the platform (p < 0.05, Figure 3A,D). In the spatial probe trial phase, we found that in the offspring PND50 and PND90, the FA-S group stayed longer in the target quadrant compared with the FA-D group (p < 0.05, Figure 3B,E) and more times to cross the platform (p < 0.05, Figure 3C,F); compared with the FA-D group, the FA-N group crossed the platform more times (p < 0.05, Figure 3C,F) and there was no significant difference between FA-N group and FA-S group. ...
Context 2
... the phase of the acquisition test, both offspring PND50 and PND90 showed that the escape latency gradually shortened with the increase of experimental days. Through repeated-measures ANOVA, we found that in the offspring PND50 and PND90, the FA-D group had the most prolonged escape latency compared with the FA-N and FA-S groups (p < 0.05, Figure 3A,D); compared with the FA-S group, the FA-N group took longer time to the platform (p < 0.05, Figure 3A,D). In the spatial probe trial phase, we found that in the offspring PND50 and PND90, the FA-S group stayed longer in the target quadrant compared with the FA-D group (p < 0.05, Figure 3B,E) and more times to cross the platform (p < 0.05, Figure 3C,F); compared with the FA-D group, the FA-N group crossed the platform more times (p < 0.05, Figure 3C,F) and there was no significant difference between FA-N group and FA-S group. ...
Context 3
... repeated-measures ANOVA, we found that in the offspring PND50 and PND90, the FA-D group had the most prolonged escape latency compared with the FA-N and FA-S groups (p < 0.05, Figure 3A,D); compared with the FA-S group, the FA-N group took longer time to the platform (p < 0.05, Figure 3A,D). In the spatial probe trial phase, we found that in the offspring PND50 and PND90, the FA-S group stayed longer in the target quadrant compared with the FA-D group (p < 0.05, Figure 3B,E) and more times to cross the platform (p < 0.05, Figure 3C,F); compared with the FA-D group, the FA-N group crossed the platform more times (p < 0.05, Figure 3C,F) and there was no significant difference between FA-N group and FA-S group. ...
Context 4
... repeated-measures ANOVA, we found that in the offspring PND50 and PND90, the FA-D group had the most prolonged escape latency compared with the FA-N and FA-S groups (p < 0.05, Figure 3A,D); compared with the FA-S group, the FA-N group took longer time to the platform (p < 0.05, Figure 3A,D). In the spatial probe trial phase, we found that in the offspring PND50 and PND90, the FA-S group stayed longer in the target quadrant compared with the FA-D group (p < 0.05, Figure 3B,E) and more times to cross the platform (p < 0.05, Figure 3C,F); compared with the FA-D group, the FA-N group crossed the platform more times (p < 0.05, Figure 3C,F) and there was no significant difference between FA-N group and FA-S group. ...
Context 5
... repeated-measures ANOVA, we found that in the offspring PND50 and PND90, the FA-D group had the most prolonged escape latency compared with the FA-N and FA-S groups (p < 0.05, Figure 3A,D); compared with the FA-S group, the FA-N group took longer time to the platform (p < 0.05, Figure 3A,D). In the spatial probe trial phase, we found that in the offspring PND50 and PND90, the FA-S group stayed longer in the target quadrant compared with the FA-D group (p < 0.05, Figure 3B,E) and more times to cross the platform (p < 0.05, Figure 3C,F); compared with the FA-D group, the FA-N group crossed the platform more times (p < 0.05, Figure 3C,F) and there was no significant difference between FA-N group and FA-S group. ...
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The effect of maternal folate status on the fetal central nervous system (CNS) is well recognized, while evidence is emerging that such an association also exists between fathers and offspring. The biological functions of telomeres and telomerase are also related to neural cell proliferation and apoptosis. The study aimed to investigate the effect...
Citations
... It can result in the altered structure and function of cells, tissues, organs, and organ systems, as well as impairments in fetal homeostasis in embryogenesis followed by persistent negative consequences at the postnatal stage of ontogenesis [1][2][3][4]. Numerous experimental studies have shown that maternal HHcy can impair brain development in a fetus or a newborn [5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23], thus affecting neurobehavioral development and cognitive functions of the offspring [5,6,10,12,15,18,20,[24][25][26][27][28][29][30][31][32][33][34][35][36][37][38]. Multiple clinical data indicate that an increased Hcy level in the mother's blood can be associated with the risk of congenital malformations of the fetal CNS [39,40]. ...
According to modern view, susceptibility to diseases, specifically to cognitive and neuropsychiatric disorders, can form during embryonic development. Adverse factors affecting mother during the pregnancy increase the risk of developing pathologies. Despite the association between elevated maternal blood homocysteine (Hcy) and fetal brain impairments, as well as cognitive deficits in the offspring, the role of brain plasticity in the development of these pathologies remains poorly studied. Here, we review the data on the negative impact of hyperhomocysteinemia (HHcy) on the neural plasticity, in particular, its possible influence on the offspring brain plasticity through epigenetic mechanisms, such as changes in intracellular methylation potential, activity of DNA methyltransferases, DNA methylation, histone modifications, and microRNA expression in brain cells. Since placenta plays a key role in the transport of nutrients and transmission of signals from mother to fetus, its dysfunction due to aberrant epigenetic regulation can affect the development of fetal CNS. The review also presents the data on the impact of maternal HHcy on the epigenetic regulation in the placenta. The data presented in the review are not only interesting from purely scientific point of view, but can help in understanding the role of HHcy and epigenetic mechanisms in the pathogenesis of diseases, such as pregnancy pathologies resulting in the delayed development of fetal brain, cognitive impairments in the offspring during childhood, and neuropsychiatric and neurodegenerative disorders later in life, as well as in the search for approaches for their prevention using neuroprotectors.
... Folate deficiencies are well known to severely impair fetal development, leading to neural tube defects (NTDs) and an increased risk of mental retardation and cretinism, respectively (Medlock & Dailey, 2022). Folic acid supplementation increased the level of folic acid, reduced the level of HCY of brain tissue in offspring and reduced the wrong incorporation of uracil into telomeres (Zhou et al., 2022). ...
The deficiency of micronutrients and anemia are nutritional problems in pregnancy. WHO in 2019 and Riskesdas in 2018 reported that anemic pregnant women in Indonesia were 44.2% and 48.9%. Public Health Service also reported there was an increased prevalence of this problem in West Sumatra and Padang City in 2019 which was 18.1% and 11.2%. Micronutrient deficiency affected the hemoglobin (Hb) level, which is one of the indicators marking anemia in pregnancy. The objective was to determine the correlation between zinc and folic acid with hemoglobin levels in the third trimester of pregnancy. This analytical cross-sectional research was held in the Health Center and laboratory of Lubuk Kilangan on May-July 2022. The population and samples were 64 third-trimester pregnant women with total sampling. Intake data were collected through interviews using the Food Frequency Questionnaire (FFQ). Hemoglobin levels were examined by a hematology analyzer. Pearson correlation was used to identify the correlation of the variables (P< 0.05) . The mean levels of zinc and folic acid intake and hemoglobin levels were 7.35 mg, 215.56 mcg, and 11.08 g/dL. There was a positive correlation between zinc (p=0,015) and folic acid (p=0.004) with hemoglobin levels in the third trimester of pregnancy.
According to modern concepts, the susceptibility to certain diseases, especially to cognitive and neuropsychiatric disorders, can be formed during the period of embryonic development. Adverse factors that affect the mother during pregnancy increase the risk of the pathology development in the postnatal period. Despite the relationship found between elevated maternal blood levels of the amino acid homocysteine (Hcy) and fetal brain formation impairments, as well as cognitive deficits in offspring, the role of brain plasticity in the development of these pathologies is still insufficiently studied. This review allows to be acquainted with the available data on the negative impact of hyperhomocysteinemia (HHcy) on the neural plasticity. An important aspect of the problem considered in the review is the possible influence of maternal HHcy on the offspring brain plasticity through the epigenetic mechanisms. Data on changes in intracellular methylation potential, activity of DNA methyltransferases, and DNA methylation in brain cells under the influence of HHcy are presented, and possible effects of HHcy on histone modifications and microRNA expression are considered. Since placenta plays a key role in the transport of nutrients and modulation of signals from mother to fetus, its dysfunction due to epigenetic mechanisms disturbances may affect the development of the fetal CNS. In this regard, the review presents data on the impact of maternal HHcy on the epigenetic regulation in the placenta. The data presented in the review are not only of theoretical significance, but are also of interest for understanding the role of epigenetic mechanisms in the pathogenesis of diseases for which HHcy is a risk factor (pregnancy pathologies accompanied by delayed fetal brain development, cognitive impairments in childhood and neuropsychiatric and neurodegenerative disorders later in life), as well as the search for approaches to their prevention using neuroprotectors.
Background:
The benefits of folic acid supplementation have been documented in several studies. However, while evidence exists regarding its benefits for growth and haematologic parameters, its possible effects on the brain have been less examined.
Objectives:
The study aimed to examine the benefits of dietary folic acid supplementation (beginning in the prepubertal period) on neurobehaviour, oxidative stress, inflammatory parameters, and neurotransmitter levels in adult mice.
Methods:
Forty-eight prepubertal male mice were assigned into four groups of 12 animals each. Mice were grouped into normal control (fed standard diet) and three groups fed folic acid supplemented diet at 2.5, 5, and 10 mg/kg of feed. Animals were fed a standard diet or folic acid-supplemented diet for eight weeks during which food intake and body weight were assessed. On postnatal day 78, animals were exposed to the open-field, Y-maze, radial arm maze, elevated plus maze, bar test, and models of behavioural despair. 24 hours after the last behavioural test, animals were made to fast overnight and then sacrificed by cervical dislocation. Blood was then taken for the assessment of blood glucose, leptin, and insulin levels. Homogenates of brain tissue were prepared and used for the assessment of biochemical parameters.
Results:
Results showed a concentration-dependent increase in body weight, and improved antioxidant status, memory scores, and acetylcholine levels. Also, a decrease in food intake, blood glucose, insulin, and leptin levels was observed. A reduction in open-field behaviour, anxiety-related behaviour, and proinflammatory markers, was also observed.
Conclusion:
The beneficial effect of prepubertal continuous dietary folate fortification on the brain (as the animal ages) has been shown in this study.
The vitamins of riboflavin and folate are necessary nutrients for maintaining the proper functioning of human body. Riboflavin and folate deficiency will cause nerve damage, leading to peripheral neuritis, depression, tongue inflammation and other diseases. The sensitive detection of riboflavin and folate keeps its significance for patients and food quality control. In this study, a quinoline-pyridine-combined chemosensor (HQ-TPE) modified by tetraphenylethene was developed. After chelating Cd2+, the chemosensor exhibited high selectivity and sensitivity for riboflavin and folate. Moreover, it can discriminate the two different vitamins through different fluorescent responses, which should arise from the different intermolecular π-π interactions between the sensor HQ-TPE and the analyte upon coordination of riboflavin or folate with Cd2+. More importantly, the chemosensor could be used in visual semi-quantitative determination of riboflavin and folate in real samples (milk and lettuce). Therefore, the sensor presented here will not only be a powerful tool for the detection of riboflavin and folate, but also provides a new template for the design of metal complex as fluorescent sensor.
