Fig 1 - uploaded by Hyo Jung Kim
Content may be subject to copyright.
Fluorescence-activated cell sorting (FACS) surface profile of bone marrow dendritic cells (BM-DCs) from BALB/c mice analysis at day 11. Mature BM-DCs express more co-stimulatory molecules (CD80, CD86), and DC markers (CD11c, DEC-205), but less or no monocyte/macrophage markers (CD14, F4/80) or granulocyte marker (anti Gr-1). Isotype control staining for hamster IgG and rat IgG2a were used to set the marker lines (straight lines).
Source publication
Dendritic cells (DCs) are potent antigen-presenting cells for the induction and activation of cytotoxic T lymphocytes. We tested whether bone marrow-derived DCs are capable of inducing protective immunity against a murine lymphoma (A20). DCs were grown from tumor-bearing BALB/c mice by culturing bone marrow cells. BALB/c mice were injected (sc) wit...
Context in source publication
Context 1
... day 11, the purity of DCs was >90% with a viability of >90%. Fig. 1 shows a representative flow cytometer result of the cellular phenotype of cultured DCs harvested on that day. Repeated flow cytometry showed cultured cells to be strongly positive for DEC-205 (88%) and CD11 (40.7%), as well as the co-stimulatory molecules CD80 (98%) and CD86 (97%). Only 7% of the cultured cells expressed F4/80, the ...
Similar publications
These studies examined the fate of Factor XIII (fibrin-stabilizing factor) in mice with plasmacytoma (MOPC-300, MOPC-384, MOPC-467, and J-558). Plasma Factor XIII levels in these mice decreased progressively with tumor expansion. No plasma inhibitors of Factor XIII activity could be detected. Factor XIII was found on plasmacytoma cell membranes and...
The Apicomplexan parasite Besnoitia caprae infects wild and domestic goats. Knowledge on Besnoitia caprae specially an optimized animal model is sparse. Experimental infections with tachyzoites of BC-Pars obtained from BALB/c mice were conducted in outbred mice to determine the infectivity and LD50 of Besnoitia caprae. Six groups of five mice were...
Immune-complex-mediated glomerulonephritis (IC-GN) was induced with daily intraperitoneal injections of horse apoferritin (HAF) for varying intervals in Swiss albino and BALB/c mice. Anti-HAF antibody avidity in plasma pools from mice with predominantly mesangial immune deposits was compared with avidity in plasma pools from mice with predominantly...
Male BALB/c mice were intraperitoneally infected with BCG vaccine. Cytomorphological changes in BCG granulomas of the liver and lungs were compared during spontaneous tuberculous inflammation and after intraperitoneal injection of dialdehyde dextran for 5 months. Administration of dialdehyde dextran to mice infected with mycobacteria of BCG vaccine...
An acetone extract of the moss Pleurochaete squarrosa was tested against eleven bacterial strains, some of which are pathogenous for man. The extract was active on some Gram-negative strains. Antibacterial activity of the extract, expressed as MICs, was compared with three reference antibiotics. Acute toxicity assay was performed in Balb C mice.
Citations
Secondary lymphoid tissue chemokine (SLC), which is expressed in T cell zones of secondary lymphoid organs, including the spleen and lymph nodes, strongly recruits both T lymphocytes and mature dendritic cells. As appropriate interaction of tumor-specific T cells and mature dendritic cells, equipped with tumor antigens, is a prerequisite for effective T cell immunity against established tumors, we mobilized lymphocytes and dendritic cells to tumor sites by intratumoral injection of secondary lymphoid tissue chemokine-Fc (SLC-Fc) fusion protein using the B16F10 murine melanoma model. Activation of dendritic cells, another prerequisite for the effective activation of naïve tumor-specific T cells, was achieved by the addition of immunostimulatory cytosine-phosphorothioate-guanine oligodeoxynucleotide (CpG-ODN) into the tumor site. Intratumoral administration of SLC-Fc or CpG-ODN revealed antitumor effects against B16F10 murine melanoma grown in the subcutaneous space. Co-treatment of SLC-Fc and CpG-ODN displayed synergistic effects in reducing the tumor size. The synergistic antitumor effect in co-treatment group was correlated with the synergistic/additive increase in the infiltration of CD4(+) T cells and CD11c(+) dendritic cells in the tumor mass compared to the single treatment groups. These results suggest that the combined use of chemokines and adjuvant molecules may be a possible strategy in clinical tumor immunotherapy.
