Figure 2 - uploaded by Alexander Boychenko
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Fluidic (A) and UltiMate 3000 RSLCnano (B) configurations for the high-throughput low-flow tandem pre-concentration LC setup. Note: The number and letter descriptions for each of the fluidic components (in black) are given in Table 1.
Source publication
Tandem high-throughput capillary-flow LC-MS based proteomics methods that permits fast sample data acquisition with maximum MS utilization time. With the modest changes to the hardware
and fluidics, MS utilization can be increased to permit constant MS sample data acquisition with high throughput and more protein group identifications. We demonstra...
Contexts in source publication
Context 1
... a novel tandem LC low-flow setup on the UltiMate 3000 RSLCnano system requires the addition of a high-pressure gradient low-flow pump module, a second 2-position, 10-port valve to the column oven, and a second separation and trap column (Figure 2). The single stack setup ensures that both the system footprint as well as the length of connecting capillaries, and hence delay volume, are minimized. ...
Context 2
... columns, and consumable accessories required to run the application. The letter and number assignments are given in Figure 2A. ...
Context 3
... were carried out using an UltiMate 3000 RSLCnano system, 3 comprising an UltiMate NCS-3500RS pump, Thermo Scientific ™ UltiMate ™ NCP-3200RS pump, and Thermo Scientific ™ UltiMate ™ WPS-3000TPL RS pulled loop autosampler ( Figure 2B). Both NCS-3500RS and NCP-3200RS pumps were configured with a ProFlow ™ flow meter. ...
Context 4
... example, the main device name was changed from "PumpModule" to "PumpModule2" to avoid a conflict in the signal names. The system fluidics were configured as shown in Figure 2A and Table 1. In order to interface the respective linear Acclaim PepMap column outlet with the 10-port valve on the right-hand side of the column oven, the fused silica outlet supplied with the columns was removed and replaced with the "nano column to MS tubing" (#5 in Table 1). ...
