Figure 1 - uploaded by Armando Reis Tavares
Content may be subject to copyright.
Flowers of 1) P. quadrangularis, 2) P. nitida, 3) P. foetida, 4) P. eichleriana, 5) P. alata, 6) P. cincinnata, 7) P. mucronata, 8) P. micropetala, 9) P. suberosa, 10) P. morifolia, 11) P. tenuifila.
Source publication
The Passiflora L. genus covers a diversity of wild species with ornamental potential, especially due to the intrinsic beauty of its exotic flowers, flowering more than once a year and the lush foliage. However, Passiflora seeds present dormancy complicating seed germination and the establishment of commercial plant production with species with high...
Similar publications
The knowledge about aspects related to seed germination are extremely important to the propagation of Passiflora species. Considering the diversity of Passiflora species and the need of studies that can elucidate the germination process of Passiflora seeds this study aimed was to evaluate methods to overcome seed dormancy in two Passiflora species...
Passiflora actinia Hooker is a passion fruit species native to Brazil, typically found in the Atlantic and Araucaria Forests. It has economic relevance (edible fruit) and medicinal value (sedative and anxiolytic properties), in addition to being used for vegetative propagation, as rootstock for other Passiflora species. This study aimed at investig...
The presence of salts in the culture medium and the addition of phytohormones, necessary to break seed dormancy in the in vitro germination of passion fruit, can induce critical values of osmotic potential. This can change the membrane permeability of seeds, affecting the hydration rate, enzyme release, ion transport, pH, and inhibitor values, whic...
Perennial medicinal and aromatic plants (MAPs) may represent interesting, environmentally friendly crops for the Mediterranean environments. Among MAPs, Passiflora incarnata L. (maypop) represents a very promising crop for its wide adaptability to diverse climatic conditions, low input requirements, and high added-value due to its unique medicinal...
Citations
... Por otro lado, algunas semillas de Passiflora experimentan fenómenos físicos y fisiológicos de dormancia que dificultan y prolongan el proceso de germinación (Marostega et al 2017). El uso de injertos como método de propagación, permitió acelerar el crecimiento y desarrollo de flores y frutos en las especies injertadas (Lee et al. 2010), lo que resulta útil para conversar in vivo o en bancos de semillas a las especies silvestres de Passiflora. ...
Las especies de Passiflora supersect. Tacsonia (Juss.) Feuillet & J.M. MacDougal son plantas adaptadas a los ecosistemas andinos que producen frutos comestibles. Estos son cultivados y comercializados a pequeña escala para su consumo como fruta fresca y para la elaboración de postres y bebidas. Ante las fuertes perturbaciones antrópicas y ambientales que sufre el hábitat natural de las especies de Passiflora L., la extinción parcial o total de ellas es una realidad latente. Por ello, con el fin de conservar estos recursos en Bancos de Germoplasma, es necesario el desarrollo de métodos óptimos para la propagación de las distintas especies. Con la intención de conservar ex situ, en la Colección de Passiflora del Banco de Germoplasma del Instituto Nacional de Innovación Agraria (INIA) a las especies de Passiflora supersect. Tacsonia, se verificó la viabilidad del uso de Passiflora edulis f. flavicarpa O. Deg. como portainjerto para la propagación asexual de seis taxa seleccionados. Passiflora tripartita var. mollissima (Kunth) Holm-Niels. & P. Jørg., P. cumbalensis (H. Karst.) Harms, P. pinnatistipula Cav., P. tarminiana Coppens & V.E. Barney, P. peduncularis Cav. y P. trifoliata Cav. var. trifoliata fueron injertados en P. edulis f. flavicarpa obteniendo un éxito de prendimiento y aclimatación óptima para todos los taxa seleccionados. Adicionalmente, todos los taxa con excepción de P. trifoliata var. trifoliata produjeron flores y frutos. Finalmente, este es el primer registro de propagación y cultivo exitoso para P. trifoliata y P. peduncularis, ambas especies endémicas de Perú, en un hábitat cercano al nivel del mar.
... O ácido giberélico (GA3), é um fitorregulador cuja principal função é controlar a divisão e alongamento celular, o que está intrinsecamente ligado à germinação e desenvolvimento das plantas, podendo estimular ou inibir a germinação (PRADO et al., 2019). Alguns autores relatam que o uso de giberelina aumenta a germinação de P. alata (FERREIRA, 1998;FERREIRA et al., 2005;SANTOS, 2015), isso foi observado por Marostega et al. (2017), em que sementes de Passiflora alata apresentaram aumento de germinação de até 24% quando tratadas com GA3 na concentração de 1000 mg L -1 . Para obtenção da massa fresca e seca, separou-se a parte área das raízes, onde as raízes foram lavadas em água corrente para retirada do excesso de substrato. ...
O presente trabalho teve como objetivo avaliar diferentes métodos de superação de dormência em sementes do maracujá doce (P. alata Curtis). O delineamento experimental adotado foi o inteiramente casualizado, com sete tratamentos e três repetições. Os tratamentos utilizados foram: T1 – testemunha; T2 – imersão em ácido sulfúrico; T3 – imersão em ácido giberélico na concentração de 1000 ppm; T4 – imersão em ácido giberélico na concentração de 3000 ppm; T5 – imersão em água utilizando a 35 °C; T6 – imersão em água a 50 °C; T7 – imersão em ácido clorídrico a 37%. Foram avaliadas a emergência, índice de velocidade de emergência, altura da plântula, diâmetro do caule, comprimento de raiz, número de folhas, massa de matéria fresca e seca. Com base nos resultados, os tratamentos utilizados não foram eficientes para superar a dormência nas sementes de maracujá doce. A imersão de sementes de maracujá doce em água a temperatura de 50 °C por 15 minutos é uma alternativa viável para a melhoria da qualidade fisiológica, sendo necessário o aprimoramento do tempo de imersão e da temperatura adequada.
... However, in seed propagation, which is one of the most widely used propagation methods, it becomes difficult to obtain seedlings due to low, slow and irregular germination (Souza et al. 2015). Some researchers have stated that the germination time of P. edulis seeds with low germination percentage varies between 10 days and 3 months, resulting in irregular seedling emergence (Delanoy et al. 2006;Marostega et al. 2017). Gutierrez et al. (2011) reported dormancy in P. edulis Sims edulis, P. ligularis and P. maliformis species. ...
... Gutierrez et al. (2011) reported dormancy in P. edulis Sims edulis, P. ligularis and P. maliformis species. Priming and stratification treatments have been used extensively in the removal of dormancy, and partial success has been achieved in some species (Marostega et al. 2017). Although some allelochem- Fig. 1 Mature Passiflora edulis Sims var. ...
Passiflora is cultivated for its edible fruits and many features such as flavor properties, aroma, high antioxidant activity in nutrient content, pharmaceutical effects, and ornamental plant feature, which has been widely used for decorative purposes in recent years. It has also been reported that seeds of Passiflora species have physical dormancy due to mechanical and chemical factors. Pre-sowing seed treatments are preferred for breaking dormancy as well as homogeneous seedling growth, increasing the emergence rate, improving seedling quality, high applicability and being economical. In this study, Passiflora edulis Sims f. edulis seeds extracted from fruits were used. Hydropriming, Ferula priming (FER), Tagetes priming (TAG), salt priming (KNO3), hormone priming (GA3) and stratification treatments were applied to the seeds before sowing. Results revealed that the highest emergence percentage (60%) and the least mean emergence time (22 days) were obtained from the stratification treatments. The emergence speed index (4.55) and coefficient of velocity of emergence (0.69) gave the highest value of the stratification treatments. In the first seedling measurements made to determine the seedling quality criteria, GA3, TAG and FER treatments, and in the final seedling measurements, stratification and FER treatments came to the fore. It is recommended that further tests on seedling quality be conducted using variable temperatures and to use it in future studies by changing the times of stratification treatments.
... Osmopriming in seeds can favor germination in numerical terms and promote repair and metabolic activation during imbibition (Santos et al., 2008). There are reports that KNO3 can stimulate germination under stress conditions and favor the metabolic routes that improve the germination process, as observed in P. eichleriana and P. setacea seeds (Marostega et al., 2017;Pádua et al., 2011). It should be noted, however, that the role of KNO3 in overcoming dormancy is controversial. ...
setacea seeds without aril, were submitted to pre-germination treatments: T1(control); T2 (pre-soaking in Promalin® solution [gibberellin (GA4+7) + N-(phenylmethyl)-1H-purine 6-amine (6-benzyladenine) both at a concentration of 18.8 g.L-1] in bainMarie, 45 °C/20 min.; T3 (osmopriming with KNO3 at a concentration of 2.6g.L-1 H2O, 25 °C/1 day, with aeration); T4 (osmopriming with KNO3 at a concentration of 2.6g.L- 1 H2O, 25 °C/2 days, with aeration); T5 (T3 followed by T2) and T6 (T4 followed by T2). The initial moisture contents, ranging from 6.7% to 8% did not differ from each other (α 0.05). The highest fresh mass increment at 168h and the highest moisture content at the end of the germination test were achieved by T2, 40.9% and 44.3%, respectively. The lowest fresh mass increment at 168h was T1 (13.1%). Osmopriming with KNO3 and aeration contributed to better water absorption in T3 and T4 than in T1, but did not stimulate the germination performance of the seeds. The highest germination percentages and germination speed indexes were obtained from T2 seeds (92% and 8, 4595), T5 (96% and 11.7438) and T6 (94% and 11.6728) as well as the lowest mean germination times T2 (11.73 days), T5 (9.49 days) and T6 (8 .81 days), which did not differ statistically from each other (α 0.05). However, the germination process took less time for T2 seeds, 21 days. The combination of aryl removal and phytohormone solution in a bain-marie at 45 °C/20min. with or without osmopriming with KNO3 stimulated the germination process of P. setacea seeds.
... Various studies have confirmed that Passiflora spp. seeds have exogenous dormancy due to a combination of mechanical and chemical factors, or even physical ones because of the impermeable tegument (Marostega et al. 2017;Angelini et al. 2021). The low emergence percentage of the species P. coccinea, P. tenuifila and P. setacea might have been caused by the presence of some type of dormancy (Table 1). ...
... The low emergence percentage of the species P. coccinea, P. tenuifila and P. setacea might have been caused by the presence of some type of dormancy (Table 1). Marostega et al. (2017) also reported the dormancy of seeds of these species and of other wild species. One of our expectations was the possibility that freezing would help to break dormancy of tegumentary origin, which did not occur. ...
Cryopreservation is an interesting strategy for the long-term conservation of the seeds of the majority of cultivated plants as well as many of the corresponding wild parents. The objective of this study was to evaluate the response of the seeds of different passion fruit species to cryopreservation, as well as to apply multivariate tools to better understand the germinative behavior after thawing, to support the application and repeatability of these methods with other Passiflora species. Seeds of P. coccinea, P. edulis, P. gibertii, P. maliformis, P. morifolia, P. setacea, P. suberosa and P. tenuifila were submitted to cryopreservation, and after thawing the following variables were analyzed: emergence percentage (%); mean emergence time (days); mean emergence speed (plantlets.day −1); uncertainty (bit); and synchrony (bit). We applied multivariate analysis of variance (MANOVA) and canonical discriminant analysis (CDA) to the data. The seeds of all the species withstood the freezing well, indicating that cryopreservation is a viable alternative for long-term conservation of Passiflora genetic material. The emergence percentage, average emergence speed and average emergence time were the variables that contributed the most to separation of the species in the first canonical discriminant function (Can1). The seeds of the species studied were found to be tolerant to desiccation because they remained viable even with low water content. The results showed that seeds could be cryopreserved successfully both with and without prior desiccation.
... Dry seeds were placed into each chemical priming treatment, thus they were placed in a 100 mL glass container and 50 mL of each chemical was added to cover the seeds, except in the case of PEG 6000, where seeds were placed in a petri dish containing two layers of filter paper and the seeds were half-soaked. The treatments that were selected based on previous studies were: pure water Type II used as control and seed remained in it for 24 h; for 48 h in hydrogen peroxide at 15% [18]; for 24 h in potassium nitrate at 1% [25]; for 36 h in PEG 6000 at −1.2 MPa [8]; and for 10 min in gibberellic acid at 500 ppm [13]. All seeds were placed in an incubator (Sansho, CN-25C, Tokyo, Japan) in dark condition at 25 °C according to the time for each treatment. ...
... It activates seed germination by creating a water potential equilibrium between seeds and the solution by osmosis; this cation can imbibe water quickly and change the water potential of the seeds [19]. Marostega et al. [25] found that ornamental passion fruit seeds treated with potassium nitrate at 1% for 24 h increased their percentage of germination; in this study, this trend was also observed in all the genotypes, except for INIAP 2009, which showed similar percentage of germination in their seeds treated with potassium nitrate and imbibed in water. ...
Seed is a fundamental tool to carry out breeding processes and for the propagation of the crops; however, seed propagation generally has low and irregular germination. Passion fruit (Passiflora) species are economically important for Ecuador, which is the main exporter of passion fruit concentrate in Latin America. Ecuadorian farmers propagate new plants by seeds to establish new passion fruit orchards or to extend their cultivated area. The objective of this research was to determine the differences in germination and seedling development with the application of priming methods in five genotypes of passion fruit belonging to three different taxa that are of commercial use in Ecuador. The genotypes used were: INIAP 2009 and P10 (P. edulis f. flavicarpa), Gulupa (P. edulis f. edulis), and local germplasms POR1 (P. edulis f. flavicarpa) and PICH1 (P. maliformis). The priming methods were: water (control), hydrogen peroxide at 15%, potassium nitrate at 1%, PEG 6000 at-1.2 MPa, and gibberellic acid at 500 ppm. The results showed that there was a genotype-response depending on the priming method. Nevertheless, Polietilenoglicol (PEG 6000) could be considered as a promising method to encourage seed germination and promote seedling growth in the Passiflora species. More research regarding the use of this compound has to be carried out in order to determine in depth the physiological processes related to its functions to improve seed germination as well as production of vigorous seedlings.
... The level of moisture in P. morifolia seeds at the beginning of the experiment was 11.99% and 1.04% in P. cincinnata. The values are similar to the values obtained by Marostega et al. (2017), where they found 9.44 and 11.47% for P. cincinnata and P. morifolia, respectively. ...
... The treatments that applied 400 and 1000 mg L -1 GA3 differed from each other, with germination values of 22 and 57%, respectively. Marostega et al. (2017) observed that the Passiflora seeds immersion in 1000 mg L -1 GA 3 for 6 hours resulted in a percentage of germination of 26% for P. nitida, 15% for P. foetida, 19% for P. eichleriana, 24% for P. alata, 8% for P. cincinnata, 86% for P. suberosa, 68% for P. morifolia and 54% for P. tenuifila. ...
The knowledge about aspects related to seed germination are extremely important to the propagation of Passiflora species. Considering the diversity of Passiflora species and the need of studies that can elucidate the germination process of Passiflora seeds this study aimed was to evaluate methods to overcome seed dormancy in two Passiflora species (P. morifolia and P. cincinnata) by pre-germinative treatments as physical scarification, immersion in gibberellic acid (GA3) and Promalin® (GA4+7 + N- (phenylmethyl) -aminopurine). To dormancy overcoming, the pre-germinative treatments were: scarification in sandpaper; immersion in water at 50 °C for 5 minutes; imbibition in 400, 1000, 2000 and 3000 mg L-1 GA3 for 5 hours; scarification in sandpaper + imbibition in 400, 1000, 2000 and 3000 mg L-1 GA3 for 5 hours; and imbibition in 0.03, 0.45, 0.90 and 2% Promalin® for 6 and 12 hours. The treatments were distributed in a completely randomized design, with four repetitions of 25 seeds. The data were submitted to variance analysis and the averages compared by Tukey test and Scott-Knott, respectively, at 5% of probability using the software SISVAR. Germinability, germination time, germination velocity, uncertainty and germination synchrony were evaluated. The results showed that 0.45, 0.90 and 2% Promalin® for 6 and 12 hours were effective to overcome the seed dormancy in P. cincinnata. The treatment 1000 mg L-1 GA3 achieved the highest result to overcome the dormancy in P. morifolia seeds.
... P. setacea seeds had a lower germination rate after six months of storage and it was attributed to a dormancy induction by low temperature [20]. Marostega et al. (2017) [21] studying P. eichleriana seed dormancy observer that 66% of the seeds germinated indicating that the overcoming of seed dormancy method was not totally effective on this species. The difference between the percentage of filled seeds and germinated seeds is due to invisible infections, physiological damage, or seed dormancy [22]. ...
... P. setacea seeds had a lower germination rate after six months of storage and it was attributed to a dormancy induction by low temperature [20]. Marostega et al. (2017) [21] studying P. eichleriana seed dormancy observer that 66% of the seeds germinated indicating that the overcoming of seed dormancy method was not totally effective on this species. The difference between the percentage of filled seeds and germinated seeds is due to invisible infections, physiological damage, or seed dormancy [22]. ...
The study aimed to evaluate the internal morphology of Passiflora eichleriana Mast., Passiflora nitida Kunth. and Passiflora mucronata Lam. seeds cryopreserved with different cryoprotectants. The treatment were control (without cryoprotectant or cryopreservation), seeds cryopreserved without cryoprotectant, 1.73, 2.28, 2.60 or 2.71 M glycerol, 0.37, 0.46, 0.54 or 0.61 M sucrose and 0.37, 0.72, 1.04 or 1.35 M dimethylsulfoxide. The seeds were cryopreserved in liquid nitrogen (-196 °C) for seven days. The cryopreserved seeds were dipped in water bath (37 °C) for 5 minutes to defrost. The percentage of filled, empty, malformed and damaged seeds was determined. The seeds were analyzed by X-ray to verify damages caused by the cryopreservation or defrost. Our results demonstrate that X-ray is an efficient method to analyses cryopreserved seeds and the cryopreservation technique did not cause mechanical damage of P. eichleriana, P. nitida and P. mucronata seeds.
... Contudo, problemas de germinação e armazenamento de sementes são muito comuns em espécies de maracujazeiro e têm se mostrado um fator limitante para os programas de melhoramento genético da cultura (Junghans, 2015;Santos et al., 2016;Marostega et al., 2017;Moura et al., 2018;Faleiro et al., 2019). Uma das formas de superação da dormência de sementes de ...
Passiflora mucronata, also known as “maracujá-de-restinga”, is among the 150 species native to Brazil and occurs in coastal environments from Bahia to the north of São Paulo. Like other wild passifloras, this species has resistance to bacteriosis, anthracnose and fusariosis, and has ornamental potential. In this work, was to evaluate the water content of the seeds, the storage period and the use of GA4+7 + BA for the conservation and overcoming of the dormancy of P. mucronata seeds were evaluated emergence of
P. mucronata.
... The seed water content was determined in an oven, using the forced air circulation method at 105 ± 3°C for 24 h, according to the Guidelines for Seed Analysis (Brasil, 2009). P. eichleriana seeds were soaked in KNO 3 solution (1%) for 24 h at 30°C, and P. nitida seeds were soaked in 1,000 mg L −1 GA 3 (gibberellic acid, 99.0%, VETEC©, Brazil) solution for 6 h at 25°C in the absence of light (Marostega et al., 2017) to overcome dormancy. P. cristalina was not submitted to any process to overcome dormancy. ...
... The achievement of sucrose treatments can be explained by their ability to maintain the crystalline liquid state of membrane bilayers and stabilize proteins under freezing conditions with no toxicity to plant cells, even when accumulated in large amounts in the cytoplasm (Carvalho and Otoni, 2010). The lower percentages of germination and emergence of P. nitida seeds may be related to seed dormancy (Marostega et al., 2017). Passiflora spp. ...
... Passiflora spp. can be further divided into two major groups according to dormancy type: chemical dormancy (responsive to chemical compounds, such as GA 3 and/or KNO 3 , and mechanical dormancy combined/or not with chemical dormancy (Marostega et al., 2017). However, no studies have reported on physical treatments of seeds such as light (Asghar et al., 2016), laser (Shafique et al., 2017) and magnetism (Kehinde et al., 2017) in relation to seed pretreatment to enhance seed germination and seedling growth. ...
This study aimed to evaluate the effects of cryoprotectant agents on Passiflora eichleriana, Passiflora crystallina and Passiflora nitida seeds undergoing cryopreservation. The experiment was conducted with non-cryoprotected or non-cryopreserved seeds (control), cryopreserved seeds without cryoprotectant and cryopreserved seeds treated with the following cryoprotectants: 1.73, 2.28, 2.60 or 2.71 mol glycerol; 0.37, 0.46, 0.54 or 0.61 mol sucrose; or 0.37, 0.72, 1.04 or 1.35 mol dimethylsulfoxide (DMSO). After treatment, the seeds were cryopreserved in liquid nitrogen (LN2) for 7 days and then defrosted in 37°C water for 5 min. The seeds were submitted to germination and vigor tests consisting of four replicates of 50 seeds. Percentage of germination (PE) and germination velocity index, percentage of emergence velocity index, shoot and root length, and shoot and root dry mass were evaluated. After cryopreservation, all of germination and vigor values were higher without the use of cryoprotectants. The cryoprotectant glycerol had a deleterious effect on seed viability and vigor for all Passiflora species. Glycerol inhibit seedling emergence and concentrations higher than 2.60 mol seed germination on P. cristalina. Higher concentrations of DMSO decrease seed viability and vigor in P. crystalina and P. nitida.
