Fig 1 - uploaded by O. Fuchiwaki
Content may be subject to copyright.
Flexible micromanipulation using versatile robots under microscopy

Flexible micromanipulation using versatile robots under microscopy

Source publication
Article
Full-text available
In this paper, we describe the development of an orthogonal microrobot for accurate microscopic operations. To conduct the microscopic operation, a simple locomotion mechanism composed of one piezoelectric actuator and two U-shaped electromagnets is proposed. The orthogonal microrobot can move precisely in one-axis with the manner of an inchworm. W...

Contexts in source publication

Context 1
... microrobots to study industrial applications where such precise microrobots provide effective benefits [12][13][14][15]. Over the last several years, we have developed versatile microrobots for micro- scopic manipulations [16]. We have also developed flexible micromanipulation organized by three versatile microrobots under microscopes as shown in Fig. 1 [17]. Here, a spherical micromanipulator is used as a microscopic manipulator. In experiments, we have demonstrated precise and flexible handling of a miniscule pipe under the good collaboration of these small robots. This manipulation device has 11 DOF with less than 100 nm resolution. We confirmed the efficiency of the microrobots. ...
Context 2
... Figure 9 is the experimental results. B 0 is the magnetic flux density when no voltage is applied. We notice that the amount of the change of magnetic flux density is proportional to the applied voltage. We also notice that the sum of right and left magnetic flux densities is constant. The approximate model of the magnetic force is explained in Fig. 10. Here, B represents the magnetic flux density and S U-shaped electromagnet Stacked type piezoelectric actuator represents the cross-section area of the magnetic core. F is the magnetic force between the magnetic core and the metal surface. In this model, we can describe approximately the magnetic force ...
Context 3
... 8 represents that the sum of magnetic force increases when electromagnets is applied a voltage. That is why the electromagnet which is applied no voltage moves although the other electromagnet which is applied a voltage is fixed. The motion sequence of this one-axis robot is depicted in Fig. 11. This simple one-axis microrobot moves like an inchworm with less than 100 nm resolution. We can make two-axial orthogonal microrobots if we connect the micro- robot to another microrobot orthogonally as Fig. 12. In this figure, the bottom robot moves itself to the X direction and the upper robot moves its steel base to the Y ...
Context 4
... moves although the other electromagnet which is applied a voltage is fixed. The motion sequence of this one-axis robot is depicted in Fig. 11. This simple one-axis microrobot moves like an inchworm with less than 100 nm resolution. We can make two-axial orthogonal microrobots if we connect the micro- robot to another microrobot orthogonally as Fig. 12. In this figure, the bottom robot moves itself to the X direction and the upper robot moves its steel base to the Y direction. We can easily set up these orthogonal microrobots in a narrow working space such as a table of an inverted microscope because the size of this robot is very small, i.e. 58 mm in length, 58 mm in width and 37 mm ...
Context 5
... can easily set up these orthogonal microrobots in a narrow working space such as a table of an inverted microscope because the size of this robot is very small, i.e. 58 mm in length, 58 mm in width and 37 mm in height. Figure 13 shows the experimental setup for measuring the displacements. We measure the robot's motion by capaci- tance sensors whose resolution is 30 nm. ...
Context 6
... the output noise is about 60 nm so the measuring resolution is 60 nm in experiments. Figure 14 is the experimental results of the relationship between the displacement and the number of steps at various frequencies. The robot moves up to 300 Hz and the step width is about 1.6 to 3.3 μm when 100 V is applied to the piezoelectric actuator. ...
Context 7
... width less than 0.1 μm can also be controlled if we control the applied voltage of the piezoelectric actuator. The robot can move with a 200 g payload. Generally, a piezoelectric actuator has a hysteretic non-linearity as depicted in Fig. 15. A distance d bp is a difference between a displacement at minimum voltage and a displacement at maximum voltage. A distance d h is a width of a hysteretic loop. The distance d h itself does not influence a repeatability of the step width directly because the orthogonal robot moves with an inchworm manner. We assume that a wire ...
Context 8
... have developed a micropump driven by the piezoelectric actuator as shown in Fig. 16. The sequence of this micro- pump is very simple. The piezoelectric actuator pushes and pulls the liquid inside the silicon tube as depicted in Fig. 17. Table 3 shows the typical performance of the micropump. This tiny pump is so small and light that the orthogonal microrobot can carry. In several experiments, we have checked this tiny ...
Context 9
... have developed a micropump driven by the piezoelectric actuator as shown in Fig. 16. The sequence of this micro- pump is very simple. The piezoelectric actuator pushes and pulls the liquid inside the silicon tube as depicted in Fig. 17. Table 3 shows the typical performance of the micropump. This tiny pump is so small and light that the orthogonal microrobot can carry. In several experiments, we have checked this tiny pump pushes 50±0.5 nl when the inner diameter of the glass pipette is 15 μm and input voltage to the piezoelectric actuator is 30 ...
Context 10
... micropump can hold an egg cell with a diameter of 100 μm by decreasing the input voltage of piezoelectric actuator. However, we could not control the discharge rate a Depending on the length of v-groove Fig. 16 Structure of the micropump of the pump when the inner diameter of injection pipette is smaller than 10 μm. Generally, inner diameter of the injection pipette should be less than a few micrometers at the artificial insemination and nuclear cell transfer. We plan to improve the sensitivity of this small ...
Context 11
... attach small manual stages to two-axial microrobots for positioning pipettes and a dish in Z direction as shown in Fig. 18. We positioned pipettes and the dish in focal height by manual Z stage in experiments. Fig. 19 is the photograph of the experimental set up and the working area for a cell processing organized by the three two-axial orthogonal microrobots on an inverted microscope. We put the holding pipette on the left robot for sample capturing. We ...
Context 12
... attach small manual stages to two-axial microrobots for positioning pipettes and a dish in Z direction as shown in Fig. 18. We positioned pipettes and the dish in focal height by manual Z stage in experiments. Fig. 19 is the photograph of the experimental set up and the working area for a cell processing organized by the three two-axial orthogonal microrobots on an inverted microscope. We put the holding pipette on the left robot for sample capturing. We also put the injection pipette on the right robot. Between these robots, we arrange another ...
Context 13
... succeeded in holding an egg cell with a diameter of 100 μm and sticking the pipette with a diameter of 5 μm into the egg cell under the good collaboration of the joystick and foot pedals' manual control and the visual feedback's automatic control. Figure 21 is the microscopic image of typical experimental operations. We believe that this manipulation device has much potential for actual use in various kinds of micromanipulation, such as an artificial insemination and a positioning of microparts of portable devices. ...

Similar publications

Article
Full-text available
The increasing demand for robotic applications in dynamic unstructured environments is a source of motivation for dexterous end-effectors which can cope with the wide variety of tasks encountered in these environments. This paper describes current research on a wireless controlled light-weight Robotic Manipulator. This mechanical arm is a self-cont...

Citations

... Robotics technologies and methodologies have been applied to microscopy in various ways. For example, a micro-nano control system has been designed for microscopic operation and has been attached to inverted microscopes for cell processing [10]. The mechanism of AFM intrinsically requires feedback control at the nanoscale and usually uses piezo-electric actuators to maintain constant contact forces. ...
... where ν ij is the correlation between (n i − m) and (n j − m). Using this, we have (10) For convenience, henceforth we will use the description 〈·〉 instead of 〈·〉 T , because we only need the expectation in the "time" domain. ...
Article
Full-text available
Single-particle electron microscopy is an experimental technique that is used to determine the 3D structure of biological macromolecules and the complexes that they form. In general, image processing techniques and reconstruction algorithms are applied to micrographs, which are two-dimensional (2D) images taken by electron microscopes. Each of these planar images can be thought of as a projection of the macromolecular structure of interest from an a priori unknown direction. A class is defined as a collection of projection images with a high degree of similarity, presumably resulting from taking projections along similar directions. In practice, micrographs are very noisy and those in each class are aligned and averaged in order to reduce the background noise. Errors in the alignment process are inevitable due to noise in the electron micrographs. This error results in blurry averaged images. In this paper, we investigate how blurring parameters are related to the properties of the background noise in the case when the alignment is achieved by matching the mass centers and the principal axes of the experimental images. We observe that the background noise in micrographs can be treated as Gaussian. Using the mean and variance of the background Gaussian noise, we derive equations for the mean and variance of translational and rotational misalignments in the class averaging process. This defines a Gaussian probability density on the Euclidean motion group of the plane. Our formulation is validated by convolving the derived blurring function representing the stochasticity of the image alignments with the underlying noiseless projection and comparing with the original blurry image.
... In the last ten years, we have developed a unique inchworm mechanism composed of four piezoelectric actuators and two electromagnets. We have developed unique applications where these small mechanisms play important roles [1] [2]. We show that small mobile mechanisms are effective in reducing the size and weight for precise instruments [3]- [6]. ...
Conference Paper
Full-text available
In this paper, we describe the dynamical analysis and improvement of velocity for a precise inchworm mechanism with 3 DOF. This mechanism is composed of 4 piezoelectric actuators and pair of electromagnets and moves like an inchworm with less than a 10 nm resolution. We calculate the dynamical relationship between 3 DOF motion and 4 piezoelectric displacements. We also calculate the maximum velocity with no slip of electromagnets because the no slip condition is very important for motion repeatability. In several experiments, we have checked the theoretical validity and we confirm that the analysis procedure works well as an initial design of the inchworm mechanism. The design procedure, basic performance, and chip-mounting applications are also discussed as an advance in the new field of micro-robotics used in precision regions.
... As a primary experiment, we have demonstrated automatic egg-cell gathering with visual feedback. Future work and the final goal are also discussed for this flexible and compact cell operation carried out by the miniature robots [7][8][9]. ...
Article
Full-text available
In this paper, we have described automatic gathering with visual feedback of egg-cells by 2 miniature robots. Generating a transgenic mouse is one of the most important operations in the bio-medical field. Sometimes scientists need several hundreds of egg-cells for only 1 experiment. However, conventional manipulators are designed for only DNA-injecting, and they are not useful devices for gathering egg-cells. To support the DNA-injecting operation, we have proposed the automatic gathering of egg-cells by 2 miniature robots and a miniature pump. In several experiments, we have checked the efficiency and confirmed that our device has good feasibility to support egg-cell processing. We have also proposed a 5 pipettes’ egg-cell processing method using miniature robots to realize multi-functional and flexible egg-cell processing.
Article
Full-text available
Miniature robots have been widely studied and applied in the fields of search and rescue, reconnaissance, micromanipulation, and even the interior of the human body benefiting from their highlight features of small size, light weight, and agile movement. With the development of new smart materials, many functional actuating elements have been proposed to construct miniature robots. Compared with other actuating elements, piezoelectric actuating elements have the advantages of compact structure, high power density, fast response, high resolution, and no electromagnetic interference, which make them greatly suitable for actuating miniature robots, and capture the attentions and favor of numerous scholars. In this paper, a comprehensive review of recent developments in miniature piezoelectric robots (MPRs) is provided. The MPRs are classified and summarized in detail from three aspects of operating environment, structure of piezoelectric actuating element, and working principle. In addition, new manufacturing methods and piezoelectric materials in MPRs, as well as the application situations, are sorted out and outlined. Finally, the challenges and future trends of MPRs are evaluated and discussed. It is hoped that this review will be of great assistance for determining appropriate designs and guiding future developments of MPRs, and provide a destination board to the researchers interested in MPRs.
Article
Miniature piezoelectric robots exhibit superior performances and have been favored by many researchers, however, there is an intractable contradiction between nano-resolution and fast speed. Inspired by motion of quadrupeds, we proposed a miniature quadruped piezoelectric robot (MQPR), which combined quasi-static motion and resonant motion to realize nano-resolution and high speed, respectively. Two theoretical models were established to determine the parameters of the proposed piezo-leg, and they were verified by experiments. A prototype with size of 35 × 44 × 12 mm³ and weight of 21.0 g was manufactured and tested. The measured results showed that the MQPR realized multi-DOF motions with resolution of less than 8.8 nm and range of about 2 μm in quasi-static motion, and achieved a linear speed of 393.5 mm/s (11.2 BL/s) and a rotational speed of 246.5 °/s in resonant motion. Moreover, a resolution of 0.31 μm was obtained by resonant motion in pulse mode, which could achieve effective cooperation of the above two motion mechanisms. In addition, the load capacity of the MQPR was 100 g, which was about 5 times its own weight. These characteristics help the MQPR have great potential to efficiently perform precision manipulations at multiple target locations over a large area, such as detection of circuit board in large integrated circuits or wafers.
Article
This study researches the use of Augmented Reality / Virtual Reality (AR / VR) in an affordable and highly flexible micro manipulation system for the recognition of microscopic images, images that support microscopic manipulation in the interface between the manipulation space and the operator. The application of AR / VR usually requires sensing of the manipulation space with more than one microscope or sensor, not only making the system more complex and costly but also making the manipulation space under the microscope smaller. In this research, therefore, we use the background finite-difference method with background models for monocular microscopic images of the manipulation space; we can build up information needed for manipulation support with AR / VR while removing the effects of any disturbance to the recognition of the manipulation space. We also use liquid bridging forces for the manipulations. The experimental results confirm that the information processing of the image processing method used in this research is adequate in both terms of accuracy and time efficiency to support microscopic manipulation using liquid bridging forces.
Article
In this paper, we describe the design and development of an insect-sized holonomic robot with nanometer resolution. To provide flexible and compact-sized microscopic operations, a unique locomotion mechanism composed of four piezoelectric actuators and two U-shaped electromagnetic legs is proposed. Here, two legs are arranged across from each other and are connected by four piezoelectric actuators so that the mechanism can move in any direction, i.e. in the X- and Y-directions as well as rotation, at a specified point in the manner of an inchworm. In the primary experiments, several performances such as positioning repeatability, resolution, and precise dexterity are checked using a CCD camera-based microscopic image tracker and a capacitive distance sensor. The identification, characteristics, design procedures, and basic performance are addressed and the biomedical, nanoscience, and chip mounting applications of this tiny holonomic robot are discussed to open up a new field of microrobotics for use in precise regions.
Article
Full-text available
In this paper, we describe the dynamical analysis and improvement of the maximum velocity within no slip of electromagnets for a 3-DOF inchworm mobile mechanism. The mechanism consists of four “Moonie” piezoelectric actuators and a pair of electromagnets and moves like an inchworm with less than 10-nm resolution. We calculate the dynamical relationship among 3-DOF motion, four piezoelectric displacements, drive frequency, magnetic force, mass of electromagnets, and spring constants of mechanical amplifiers. We also calculate the maximum velocity with no slip of electromagnets because the no-slip condition is significant for positioning repeatability. In several experiments, we have checked the theoretical validity and positioning repeatability. The design procedure, basic performance, and chip-mounting applications are also discussed for cultivating flexible, compact, low-inertia, and omnidirectional precise positioning technology.
Article
In this paper, an automatic control system with a force sensor and a small force input device is presented for an orthogonal-type robot. The automatic control system is composed of a force feedback loop, a position feedback loop and a position feedforward loop. The force feedback loop controls the resultant force consisting of tool contact force and kinetic friction forces. The stability criterion of the force control system is briefly discussed. In addition, the position feedback loop controls the position in only pick feed direction. The position feedforward loop guides the tool tip along a desired trajectory called cutter location data (CL data), in which the feed rate is suitably generated by a fuzzy reasoning according to each model's curvature. Furthermore, a fine stick-slip motion control strategy is added to improve the lapping efficiency. The fine stick-slip motion is orthogonally generated to the direction of tool movement. Finally, a force input device is designed to enable an operator to manually regulate the desired feed rate or the desired polishing force. The force input device allows the robot to realize cooperative motion between the automatic control and the manual control reflecting operator's skill. The effectiveness of robot's performance is examined through experiments.
Conference Paper
Full-text available
In this paper, we describe the design and development of a positioning and compensation device for a versatile micro robot. To provide microscopic operations, the versatile micro robot has developed. The robot can move in any direction in the manner of an inchworm. However many positioning errors exist because of an assembling error in the four piezoelectric actuators and two electromagnets. We need an automatic positioning device for more accurate work. Motion compensation is also highly required for more effective work. This robot is very small, so it can easily be set to work with the various devices. The positing device should also be small so that we can carry the device easily and set it up in a narrow working area. In this report, we propose a USB camera based positioning device with special compensation function. In experiments, we succeeded in controlling the robot on an octagonal path with controlling the posture angle freely. When we compensate for the motion, the positioning time becomes 60 % compared to that of no compensation. The design procedure, basic performance and biomedical application are also discussed as an advance in the new field of micro-robotics used in precision regions.