Figures 2 - uploaded by Ali Çınar
Content may be subject to copyright.
A-2D. A: control group showing the femoral growth plate and the bone trabeculae with a normal histological appearance (-)), B: resveratrol group showing the femoral growth plate and the bone trabeculae with a normal histological appearance (-), C: NaF group showing thinning of the bone trabeculae (++) and a reduced cellular density in the femoral growth plate of femur (+++), and D: NaF +resveratrol group showing a slight thinning of the bone trabeculae (+) and a slightly reduced cellular density in the femoral growth plate of femur (+). H & E staining, bar=20 µm. Scoring of histopathological findings:-(negative), + (slight), ++ (moderate), and +++ (severe).
Source publication
This study was carried out to determine the protective effects of resveratrol
on some biochemical parameters and histopathological findings in experimentallyinduced
chronic fluorosis in rats. Forty male Wistar albino rats, weighing 250–300 g,
were randomly divided into four groups of 10 rats and treated for 12 weeks as follows:
(i) control group (d...
Citations
... In recent years, although studies are examining the effect of BG on nephrotoxicity (14), wheatgrass on renal failure (15) and diabetes (10); the antioxidant effect of borax (16), boric acid (17), resveratrol (18,19) there is no study about the effect of barley grass on TOS, TAS, and DNA damage glucose and hemoglobin A1c (HbA1c) in diabetic rats. Therefore, we investigated the effects of barley grass on these parameters. ...
Objective: Worldwide, phytotherapy methods acquire great importance, and studies in this field are increasing their importance each day. This study, it was aimed to examine total antioxidant (TAS), oxidant status (TOS), oxidative DNA damage (8OHdG), glucose and hemoglobin A1c levels and the effects of barley grass (BG) on these parameters in rats with diabetes mellitus (DM). Materials and Methods: Four groups were formed for the study and 6 male rats weighing 250-350 g were used in each group. Control (C) group; physiological saline was injected via i.p., DM group; created using streptozotocin (STZ), BG Group; Barley grass juice (3 ml/rat/day) was given orally for 4 weeks, DM+BG Group; was injected STZ and BG was given by oral for 4 weeks. Results: It was determined that barley grass juice decreased blood sugar, glucose, HbA1c, TOS and OSI values, increased TAS value and body weights in STZ-induced diabetes. In addition, it was determined that the addition of BG provided significant protective effect and improvement on these parameters. Conclusion: Based on these findings, we can say that barley grass has an antidiabetic-antioxidant effect and weight gain in diabetes mellitus.
... Steatosis in liver generally depends on excess nutrition, accumulation of extracellular proteins and glycogen as well as infectious, oxidative stress, toxic and hypoxic reasons (Yildirim, 2017;Yildirim et al., 2018;Yildirim, Oto, Comba, Ekin, & Cinar, 2017). ...
This research was designed to determine the effects of rosemary (Rosmarinus officinalis ) extract on growth, blood biochemistry, immunity, antioxidant, digestive enzymes and liver histopathology of rainbow trout. In a 40‐day diet experiment, rainbow trout was fed with the experimental diets containing 0.4, 0.7, 1 and 3 g/kg of rosemary. 0.4 and 0.7 g/kg rosemary extracts had no significant effect on all parameters studied (p > .05). The growth performance (SGR and WG) and feed utilization (FCR and FER) of the fish increased with the increase in the rate of rosemary in the feed (p < .001). Additionally, only the final weight levels were significant at the linear level (p < .05). Rosemary's 1 and 3 g/kg doses increased total protein, trypsin, amylase, lipase, superoxide dismutase (SOD), catalase (CAT), lysozyme, total immunoglobulin (IG) and white blood cell (WBC) levels when compared to control group. However, glucose, aspartate aminotransferase (AST), alanine aminotransferase (ALT), total cholesterol and triglyceride levels decreased significantly (p < .001). Moreover, these doses of rosemary caused a significant reduction in steatosis of liver hepatocytes compared to the control group. In conclusion, the present study showed that rosemary may be useful in reducing the stress effects by increasing the growth, antioxidant, digestive enzymes and immunity levels, as well as reducing the rate of steatosis in the liver of rainbow trout.
... 10 Nowadays a specific concern has been offered to the utilization of dietary antioxidants of natural products as an alternative medical therapy that may have several pharmaceutical benefits. 11 Resveratrol (RSV) (trans-3,5,4′-trihydroxy-transstilbene) is a natural polyphenol component present abundantly in grapes and red wine as well as in some of the herbal remedies. 12 It possesses many beneficial biological and pharmacological properties, such as neuroprotective, anti-inflammatory, anticancer (such as thyroid, prostate, breast, stomach, colon, lung, and pancreas cancers), and antioxidant actions. ...
Lithium (Li) is used for the treatment and prophylaxis of mental disorders, associated with many serious hazards. Resveratrol (RSV) has various beneficial therapeutic effects. Platelet-rich plasma (PRP) is a new promising curative tool. This study aimed to assess the impacts of RSV versus PRP on lithium-induced thyroid follicular cell toxicity in adult male rats. Forty-nine adult male rats were divided into four groups: group I: control rats; group II: lithium-treated rats; group III: lithium- and resveratrol-treated rats; group IV: lithium and PRP-treated rats. Thyroid specimens were taken and processed for histological and immunohistochemical methods. Morphometrical studies and statistical analysis were done. Group II showed distorted thyroid follicles, significantly increased collagen fibers, and highly positive P53 immunostaining (P < 0.01). Ultrastructural examination showed dilated rough endoplasmic reticulum and damaged mitochondria. Groups III and IV exhibited significant amelioration of the histological and electron microscopic changes mentioned previously. PRP remedy was more effective than RSV for treatment of Li-induced thyroid follicular cell toxicity.
... A lot of theories have been forwarded to explain the mechanism of aluminum or sodium fluoride intoxication including the free radicals and oxidative stress with disturbances in the antioxidant defense system and as consequences disturb cellular nucleotide and induce metabolic disorder (Mailloux, Lemire, & Apana, 2011;Pawłowska-Goral, Kurzeja, & Stec, 2013;Viezeliene, Jansen, Rodovicius, Kasauskas, & Ivanov, 2011). Yildirim et al. (2017Yildirim et al. ( , 2018 showed that the chronic exposure to NaF in the drinking water caused a significant increase in the activities of AST, ALT, and the levels of creatinine and MDA, whereas the levels of GSH in the liver was significantly decreased, and these finding are consistent with our data. They attributed the hepatic and renal functions disturbance to the oxidative stress of these tissues to the response the liberated of the reactive oxygen species (ROS) that caused the degeneration and necrosis of the hepatocytes caused as a result of exposure to NaF. ...
Background: This work was designed to evaluate the potential hazards of sodium fluoride (NaF) and aluminum chloride (AlCl 3) given separately or in conjugation throughout the prenatal and up to weaning time or till the postnatal 70th day. The levels of the following parameters were then assessed; vitamin C (ascorbic acid), glutathione (GSH) and oxidized glutathione (GSSH), malondialdehyde (MDA), total protein, albumin, total calcium, ionized calcium, creatinine, urea, uric acid, and bilirubin. In addition, the activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were determined. In this study, female pregnant rats were allocated into four groups. The first received the drinking deionized water and served as a normal group, the second was given a daily dose of NaF (0.15 g/L) dissolved in deionized water from day 6 of gestation till the end of the weaning period, and the third was given a daily dose of AlCl 3 (500 mg/L) for the same period of time. The fourth group was given drinking water containing combined doses of NaF + AlCl 3 for a similar length of time. Each group was further divided into two subgroups; the first continued to be treated with the same pollutants in drinking deionized water at the same dose level until the age of 70 days, whereas the second group was supplied with pure deionized water free from the intoxicating substances for the same period of time.
... üksek florür konsantrasyonları çevreye zararsız olmasına rağmen canlılar tarafından akut ve kronik bir biçimde alınması insan ve hayvan sağlığını olumsuz etkileyebilmektedir (1)(2)(3)(4). Kronik florozis hayvansal üretimde büyük ekonomik kayıplara neden olabilmektedir. Yüksek miktarda flor alımı, iskelet, dişler ve yumuşak dokular üzerinde mineralizasyon sürecine etki eder ve genellikle geri döndürülemez olan kusurlar oluşturmaktadır (5,6). ...
... This could be due to its efficiency in removing F − from the system as reported from our previous studies (Ameeramja et al. 2016;Ameeramja and Perumal 2018). Our results revealed a significant decrease in RBC on exposure to F − with a decrease in HCT which is not significant as corraborated with the earlier findings (Atmaca et al. 2014;Yildirim et al. 2017). The decreased HCT may be due to decreasd RBC count with increased cell size. ...
Fluoride (F−) is becoming an ineluctable environmental pollutant causing deleterious effects in humans. In the present study, we examined whether tamarind seed coat extract (TSCE) is beneficial against the F−-induced systemic toxicity and hematological changes. Wistar rats were randomly grouped as follows: group I served as control; group II intoxicated with sodium fluoride (NaF, 300 ppm) in drinking water; group III was administered through oral intubation with TSCE (100 mg/kg bw); group IV was
treated with NaF (300 ppm) in association with TSCE (100 mg/kg bw) for 30 days. The results indicated that F− exposure induced oxidative stress as evidenced by elevated levels of reactive oxygen species and lipid peroxidation in the brain, liver, and kidney.
F− administration modulates hematological indices—WBC, RBC, and mean corpuscular volume. Moreover, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, acetylcholinesterase, and monoamine oxidase significantly increased
on F− exposure. Conversely, δ-aminolevulinic acid dehydratase and glutathione/reduced glutathione ratio were decreased. Activity of antioxidants—superoxide dismutase, catalase, glutathione peroxidase, and vitamin C—was also significantly decreased
due to F− administration. Treatment with TSCE effectively mitigated the alterations through its antioxidant potential. The data suggested that the TSCE had beneficial effects in alleviating the F−-induced toxicity and hence can serve as a promising neutraceutical agent.
Objective: In this study, it was aimed to investigate the effects of nettle seed, grape seed and flax seed added to the broiler diet on body weight, electrocardiogram, hematological and histopathological parameters. Materials and Methods: In this study 40 daily Ross 308 female broilers were used. Chicks were divided into 4 groups of 10 chicks each. During 42 days, in addition to standard broiler feed, nettle seed, grape seed and flax seed with 30 gr/kg/day were added to group II, III, IV, respectively. The values of erythrocyte (RBC), leukocyte (WBC), platelet (PLT), hematocrit (HCT), hemoglobin (Hb) and percentages of white blood cell were determined by conventional methods. The liver, kidney, spleen and lung tissues held in formaldehyde (10%), were passed through the alcohol and xylose serial in routine tissue tracking and were buried in paraffin blocks. They were prepeared on the lam and stained with Hematoxylin-eosin (HE) and examined by light microscopy. Results: It was determined that body weight was higher in control group than those in the other groups in the second weighing and there was a decrease in the number of heart breath in the group IV (p≤0.05). There was no significant difference with regard to hematological and histopathological findings. Conclusion: Even if the additionally nettle seed, grape seed and flax seed (30 mg/kg) to broiler rations did not have any positive effect on body weight gain, it is also important that it has no negative effect on blood, heart, liver, kidney, lung and spleen.
In this study, the effects of resveratrol (RES) on leukocyte percentages, total oxidant (TOS) -antioxidan status (TAS) and DNA damage (8-OHDG) were investigated in rats induced by methotrexate (MTX). The rats used in the study were randomly divided into 4 groups each consisting of 10 rats.Control group , MTX group (20 mg / kg ip, once a week, for two weeks) ,RES group (40 mg / kg / day orally, for two weeks), MTX + RES group (20 mg / kg ip, once a week and 40 mg / kg / day orally for 2 weeks)At the end of the experiment, blood samples were taken from the hearts of the rats and the percentage of leukocyte (neutrophil, eosinophil, basophil, lymphocyte, monocyte) was examined by classical methods. The levels of TOS and TAS were measured by colorimetric kits and 8-OHDG level by ELISA kit. Neutrophil (p≤0.05) and monocyte (p≤0.01) percentages of MTX and MTX + RES groups were found to be statistically lower than control and RES groups. There was also a significant increase in TOS and OSI levels (p
Context:
Diabetes is a metabolic disorder related to blood insulin deficiency and high glucose level. Cinnamaldehyde is an important component of cinnamon and has an effect on blood glucose.
Objective:
It was aimed to investigate the the effect of cinnamaldehyde on the liver glutathione (GSH), glucose-6-phosphate dehidrogenase (G6PD) activity, blood glucose, protein, lipid and erythrocyte parameters, live weight in diabetic rats.
Material and methods:
Rat used for this research were divided 4 group as control, diabetic, cinnamaldehyde and diabetic + cinnamaldehyde group. The live weight and fasting blood glucose level, taken from tail vein were recorded every ten days. End of the trail the blood samples were taken from rats. Biochemical parameters with autoanalyzer and hematological parameters with blood cell counter were determined in blood. The activity of G6PD and GSH amounts were measured with ELISA in the liver tissues.
Results:
Blood sugar, triglyceride, total cholesterol, VLDL, LDL, and urea levels increased in diabetic rats, and cinnemaldehyde significantly decreased these parameters. Cinnemaldehyde also showed a positive effect on body weight, blood total protein, and mean corpuscular volume in diabetes. A decrease in HbA1c and an increase in liver G6PD, GSH activity were found in treatment group, but these changes were not statistically significant.
Conclusion:
In conclusion, the antidiabetic, hypolipidemic and antioxidant effects of cinnamaldehyde were determined. It has also been shown to improve anemia, ürea levels and weight loss.
