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Box plots for anatomical characters of the five Monacha clades investigated. The lower and upper limits of the rectangular boxes indicate the 25th to 75th percentile range, and the horizontal line within the boxes is the median (50th percentile).
Source publication
Molecular analysis of nucleotide sequences of mitochondrial cytochrome oxidase subunit 1 (COI) and 16S ribosomal DNA (16SrDNA) as well as nuclear histone 3 (H3) and internal transcribed spacer 2 of rDNA (ITS2) gene fragments together with morphological analysis of shell and genitalia features showed that English, French and Italian populations usua...
Citations
... The anatomy of the genital tract of M. campanica is not adequately illustrated, lacking adequate comparisons with M. cantiana and M. pantanellii (De Stefani, 1879) and genetic data, that could confirm the taxon as a valid species (cf. Pieńkowska et al., 2018Pieńkowska et al., , 2020. Paulucci, 1881 ( Fig. 37 fig. 3 c); -la sua spira è più depressa che nelle varietà setulosa e Calabrica, forma globosa, (l. ...
The taxa established by Marianna Panciatichi Ximenes d’Aragona Paulucci are preserved at the Natural
History Museum of the University Museum System of the University of Florence. The examination of the
analytic revision of the types follows the first two papers already published (Cianfanelli et al., 2021, 2023).
In this third part, 49 taxa are examined and, following the criterion of dealing them in chronological order
of publication as defined in the first part, the subsequent four publications by Paulucci are taken into
account (Paulucci, 1880a, 1880c, 1881a, 1881b). The taxa analysis follows the same outline of the first
part (Cianfanelli et al., 2021) with an analytic sheet for each taxon, divided into paragraphs (Original
publication, Original description, Type locality, Type material, Current status, Remarks) and with the
reproduction, for each taxon, of the high resolution photos of a sample of the typical series in the different
main views. Other taxa mentioned in literature, erroneously attributed to Paulucci, other ones established
by Paulucci considered as non-valid and others established by Paulucci, considered as nomina nuda, are
also reported
... Our previous research on several M. cantiana populations, using an integrative approach combining analysis of the shell structure and genital anatomy with that of nucleotide sequences of mitochondrial and nuclear gene fragments, revealed six lineages, namely CAN-1, CAN-2, CAN-3, CAN-4, CAN-5 andCAN-6 (Pieńkowska et al. 2018b, 2019a). CAN-1 (representing true M. cantiana) was found to occur in the Latium region of Italy and in Spain and Britain (Pieńkowska et al. 2018b;Čejka et al. 2020), in line with the suggestion that this lineage probably spread with the Roman conquests (Pieńkowska et al. 2018b). Populations of CAN-2 were found in regions of Italy (Emilia Romagna) north of Latium (Pieńkowska et al. 2018b) and somewhat surprisingly in Slovakia (Bratislava) (Čejka et al. 2022), while those of CAN-3 were reportedly widespread even further north in Italy (Friuli-Venezia Giulia) as far as Vienna in Austria (Pieńkowska et al. 2018b(Pieńkowska et al. , 2019b and Bratislava in Slovakia (Čejka et al. 2022). ...
... Our previous research on several M. cantiana populations, using an integrative approach combining analysis of the shell structure and genital anatomy with that of nucleotide sequences of mitochondrial and nuclear gene fragments, revealed six lineages, namely CAN-1, CAN-2, CAN-3, CAN-4, CAN-5 andCAN-6 (Pieńkowska et al. 2018b, 2019a). CAN-1 (representing true M. cantiana) was found to occur in the Latium region of Italy and in Spain and Britain (Pieńkowska et al. 2018b;Čejka et al. 2020), in line with the suggestion that this lineage probably spread with the Roman conquests (Pieńkowska et al. 2018b). Populations of CAN-2 were found in regions of Italy (Emilia Romagna) north of Latium (Pieńkowska et al. 2018b) and somewhat surprisingly in Slovakia (Bratislava) (Čejka et al. 2022), while those of CAN-3 were reportedly widespread even further north in Italy (Friuli-Venezia Giulia) as far as Vienna in Austria (Pieńkowska et al. 2018b(Pieńkowska et al. , 2019b and Bratislava in Slovakia (Čejka et al. 2022). ...
... CAN-1 (representing true M. cantiana) was found to occur in the Latium region of Italy and in Spain and Britain (Pieńkowska et al. 2018b;Čejka et al. 2020), in line with the suggestion that this lineage probably spread with the Roman conquests (Pieńkowska et al. 2018b). Populations of CAN-2 were found in regions of Italy (Emilia Romagna) north of Latium (Pieńkowska et al. 2018b) and somewhat surprisingly in Slovakia (Bratislava) (Čejka et al. 2022), while those of CAN-3 were reportedly widespread even further north in Italy (Friuli-Venezia Giulia) as far as Vienna in Austria (Pieńkowska et al. 2018b(Pieńkowska et al. , 2019b and Bratislava in Slovakia (Čejka et al. 2022). The lineage CAN-4, corresponding to Monacha cemenelea (Risso, 1826), was found in south-eastern France (Pieńkowska et al. 2018b;Čejka et al. 2020). ...
Features of shell and genitalia as well as nucleotide sequences of selected mitochondrial and nuclear genes of specimens of Monacha cantiana from ten northern French and two Dutch populations were compared with the same features of British and Italian populations. They were found to be very similar to populations previously identified as belonging to the CAN-1 lineage of M. cantiana. This confirms previous suggestions that M. cantiana was introduced to western Europe (England, France and the Netherlands) in historical times.
... M. claustralis is now spreading quickly northward (Pinter 1968;Hlaváč & Peltanová 2010;Pieńkowska et al. 2015Pieńkowska et al. , 2016Pieńkowska et al. , 2018aHutchinson et al. 2019;Čejka et al. 2020;Gural-Sverlova & Gural 2022) from its native range in European and Anatolian Turkey (Hausdorf 2000a) and Greece (with Corfu/Kerkyra as type locality, Welter-Schultes 2012). M. cantiana is found in Great Britain, northern France and Germany, in the Benelux countries as well as in Spain, where it was probably introduced in Roman times from its native area in central Italy (Kerney et al. 1964;Kerney 1970;Evans 1972;Pieńkowska et al. 2018b). Monacha (Platytheba) ocellata (Roth 1839), known from the vicinity of Istanbul in Turkey, was recently discovered in a single locality in Britain, probably resulting from passive introduction in unknown circumstances (Anderson et al. 2018). ...
... The material is kept in the collection of the Department of Cell Biology, Adam Mickiewicz University, Poland (DCBC), the Małgorzata Proćków collection (MNHW; Museum of Natural History, University of Wrocław, Poland) and the Folco Giusti collection (FGC; Dipartimento di Scienze Fisiche, della Terra e dell'Ambiente, Università di Siena, Italy). The material used for comparison has already been described (see Pieńkowska et al. 2018b : table 1, 2019a: table 1, 2020: table 1). ...
... For the H3 gene, GenBank only contains sequences of the M. cantiana s.l. complex, deposited in connection with our previous papers (Pieńkowska et al. 2018b(Pieńkowska et al. , 2019a(Pieńkowska et al. , 2020. We therefore present two trees, based on various analyses of single or multiple locus data sets, one consisting of the ITS2 gene sequences cut off from flanking fragments ( Figure 24) and the other built from concatenated ITS2 + H3 sequences ( Figure 25, Supplementary Material Table S2). ...
Populations of Monacha atacis from southern Occitania in France and of M. samsunensis from northern Anatolia in Turkey
(Atakum/Samsun and Kastamonu) were investigated by an integrative approach based on morphological (shell and
genitalia) and molecular (mitochondrial and nuclear gene sequences) features. Morphological examination revealed a
complex pattern of variation within and between geographically separated populations, while molecular analysis showed
strong similarity between the two species, confirming earlier suggestions that the species are conspecific. Pfeiffer’s name
Helix samsunensis introduced in 1868 has priority over the name M. atacis given by Gittenberger & de Winter in 1985.
... & Neubert, 2017;Neubert et al., 2019), the "variety" M. o. umbrica is not mentioned by any other study since its establishement. The genus Monacha needs an updated revision for the numerous taxa described in the past from the Italian Apennines (Pieńkowska et al., 2018(Pieńkowska et al., , 2019. As it is therefore not possible to validate the taxon at a specific level, nor to establish its possible synonymy, it is considered, pending future studies, as taxon in quirendum. ...
After the publication of the first part (Cianfanelli et al., 2021), the catalogue of taxa established by Marianna Paulucci Ximenes d’Aragona Panciatichi continues. In this second part, 35 taxa are examined. Following the chronological order of publication, two manuscripts from 1879 (Paulucci, 1879a, 1879b) and the subsequent ones published in two years (Paulucci, 1879–1880) are taken into account. The taxa analysis follows the same outline of the first part (Cianfanelli et al., 2021) with an analytic sheet for each taxon, divided into paragraphs (Original publication, Original description, Type locality, Type material, Current status, Remarks) and with the reproduction, for each taxon, of the high resolution photos of a sample of the typical series in the different main views. Other taxa mentioned in literature, erroneously attributed to Paulucci, other ones established by Paulucci considered as non-valid and others established by Paulucci, considered as nomina nuda, are also reported.
... Continuing work on the hygromiid Monacha (Pieńkowska et al. 2015(Pieńkowska et al. , 2016(Pieńkowska et al. , 2018a(Pieńkowska et al. , 2018b(Pieńkowska et al. , 2019a(Pieńkowska et al. , 2019b, we studied species living in the mountain grasslands of the central Apennines, whence came reports of three species, the widespread M. cantiana (Montagu, 1803) and the endemic M. orsini (Villa and Villa, 1841) and M. ruffoi Giusti, 1973, and a number of taxa with uncertain taxonomic status (Alzona 1971;Manganelli et al. 1995). We conducted a joint molecular and morphological study of many populations, finding many different species or their molecular lineages. ...
... Ten populations of Monacha pantanellii (Table 1, Fig. 1) were considered in our analysis of their molecular and morphological (shell and genitalia structure) variability, and compared with the M. cantiana s. l. lineages (Pieńkowska et al. 2018a(Pieńkowska et al. , 2019b. The sequences deposited in GenBank were also considered for the molecular analysis (Table 2). ...
... The material is kept in the F. Giusti collection (FGC; Dipartimento di Scienze Fisiche, della Terra e dell'Ambiente, Università di Siena, Italy). The material used for comparison has already been described (see Pieńkowska et al. 2018a : table 1, 2019b: table 1). ...
Specimens obtained from ten populations of a Monacha species from the central Apennines were compared with six molecular lineages of Monacha cantiana s. l. (CAN-1, CAN-2, CAN-3, CAN-4, CAN-5, CAN-6) and two other Monacha species (M. cartusiana and M. parumcincta), treated as outgroup, by molecular (nucleotide sequences of two mitochondrial COI and 16S rDNA as well as two nuclear ITS2 and H3 gene fragments) and morphological (shell and genital anatomy) analysis. The results strongly suggest that these populations represent a separate species for which two names are available: the older Helix pantanellii De Stefani, 1879 and the junior M. ruffoi Giusti, 1973. The nucleotide sequences created well separated clades on each phylogenetic tree. Genital anatomy included several distinctive features concerning vaginal appendix, penis, penial papilla and flagellum; instead, shell characters only enabled them to be distinguished from M. cartusiana and M. parumcincta. Remarkably, populations of M. pantanellii show high morphological variability. Shell variability mainly concerns size, some populations having very small dimensions. Genital variability shows a more intricate pattern of all anatomical parts, being higher as regards the vagina and vaginal appendix. Despite this morphological variability, the K2P distance range of COI sequences between populations is narrow (0.2-4.5%), if we consider all but three of the 53 sequences obtained. This research confirmed that the species of Monacha and their molecularly distinguished line-ages can only occasionally be recognised morphologically and that they have significant inter-and intra-population variability. The possibility of using an overall approach, including shell, genital and molecular evidence, was taken in order to establish a reliable taxonomic setting.
... On the contrary, the distinction between Gomphroa and Hypnophila is also supported by some shell and genital features (ManGanelli et al. 2019). We have always stressed (Pieńkowska et al. 2018) that molecular features alone are insufficient to make taxonomic conclusions but that they must be supported by morphological and anatomical features. Thus any taxonomic conclusion concerning the relationship between the genera Gomphroa and Hypnophila seems to be premature. ...
Analysis of nucleotide sequences of mitochondrial COI and nuclear 5.8S+ITS2+28S gene fragments was performed on newly obtained specimens of Hypnophila pupaeformis. The results partially agree with previous morphological (shell and genitalia) analysis. They support separateness of H. pupaeformis from all species assigned to Gomphroa, Cryptazeca, Hypnocarnica and Azeca. They also show close relationships of H. pupaeformis with the Gomphroa group. Indeed Hypnophila and Gomphroa form a clade consisting of four subclades: Hypnophila and three lineages named provisionally Gomphroa A, Gomphroa B and Gomphroa C. However, more research is needed to determine their relationships and to establish whether Hypnophila and Gomphroa are two genera or two (or even four) subgenera of one genus.
... (Montagu, 1803) is widely distributed in Europe (Welter-Schultes 2012). Examina on of several English, Italian, Austrian and French popula ons showed that they consisted of at least six cryp c lineages (CAN-1 -CAN-6), some of which might deserve dis nct taxonomic status (Pieńkowska et al. 2018(Pieńkowska et al. , 2019a. ...
... The rate varia on model allowed some sites to be evolu onarily invariable [+I]. Bootstrap analysis was run with 1000 replicates (Felsenstein 1985 DNA extrac on, amplifi ca on, sequencing methods and phylogene c inference are described in detail in our previous papers (Pieńkowska et al. 2018(Pieńkowska et al. , 2019a. ...
... For anatomical analysis of genital structure, snail bodies from fi ve specimens from each new Viennese popula on were dissected under a light microscope (Wild M5A or Zeiss SteREO Lumar V12). Anatomical structures were drawn using a Wild camera lucida (for details see Pieńkowska et al. 2018Pieńkowska et al. , 2019a. ...
Monacha cantiana s.l., a species not native to Austria, was first recorded in northern lower Austria in 1995 and later in Vienna and surroundings. Its distribution continues to spread. To clarify the origins of this introduced species, some Viennese populations of M. cantiana s.l. were compared with other M. cantiana lineages using the mitochondrial
cytochrom c oxidase subunit 1 (COI) and 16SrRNA (16S) gene sequences. Genital structures were dissected to find anatomical traits that could distinguish different species or genetic lineages. A Maximum Likelihood tree placed the Austrian specimens in a clade of north Italian populations. Specimens from both areas showed idencal morphology of anatomical structures. Phylogenetic analysis showed that the Austrian-north Italian clade was closely related to the French Monacha cemenelea, but not to M. cantiana s.str.. More comprehensive studies using molecular genetic markers and anatomical
traits may be able to determine whether or not the Austrian-north Italian clade is conspecific with M. cemenelea.
... (Montagu, 1803) is widely distributed in Europe (Welter-Schultes 2012). Examination of several English, Italian, Austrian and French populations showed that they consisted of at least six cryptic lineages (CAN-1 -CAN-6), some of which might deserve distinct taxonomic status (Pieńkowska et al. 2018(Pieńkowska et al. , 2019a. ...
... 3). DNA extraction, amplification, sequencing methods and phylogenetic inference are described in detail in our previous papers (Pieńkowska et al. 2018(Pieńkowska et al. , 2019a. ...
... For anatomical analysis of genital structure, snail bodies from five specimens from each new Viennese population were dissected under a light microscope (Wild M5A or Zeiss SteREO Lumar V12). Anatomical structures were drawn using a Wild camera lucida (for details see Pieńkowska et al. 2018Pieńkowska et al. , 2019a. ...
Arianta 7 is the first volume of Arianta published in form of the Society Journal of MoFA (Mollusc Research Austria). Arianta was first published in 1995 as a booklet containing
the documentation of a Workshop on Alpine land snails which had taken place in July 1994 in Johnsbach, Styria. It was followed by five more issues which were irregularly
published. All volumes are available via the webpage of MoFA https://www.molluskenforschung.at.
We are happy to present Arianta 7 in a new layout and with a diverse content including original articles, excursion reports as well as the abstracts of the first MoFA Meeting held on 26th and 27th June 2019 in Salzburg, Austria. In this volume you find also the actual instructions for authors. The aim of MoFA is to foster malacological research, to strengthen the scientific malacological network in Austria and to support co-operations with related societies, working groups and researchers nationally and internationally. In this context we regard Arianta as an important medium to publish reports, interesting novel observations, species lists, pilot studies and field studies, besides larger original articles. But it can also be considered as suitable platform for book reviews and scientific
announcements. Since the journal is freely available online, the Arianta publications should well contribute to the aims of the Society MoFA and to the communication in malacological research in general. We are looking forward to many future issues of Arianta and we invite all readers to submit their work to our journal.
Elisabeth Haring President of MoFA, Editor of Arianta
Helmut Sattmann Editor of Arianta
Robert A. Patzner Editor of Arianta
... Monacha cantiana, as well as H. cerioi, were also considered originally uncommon in Capri Island by Bellini (1900a); Petraccioli et al. (2005Petraccioli et al. ( , 2006 did not find it during recent samplings held in Capri Island, and only listed Monacha cartusiana (Müller, 1774) as present. Noteworthy, species-level taxonomy of the genus Monacha Fitzinger, 1833, and particularly of the M. cantiana species complex, is still unresolved (Welter-Schultes 2012; Neiber & Hausdorf 2017;Pieńkowska et al. 2018Pieńkowska et al. , 2019. Apart of that, the absence of type material and even figures in any paper published by Bellini, as well as the fact that the description originally offered by Bellini (1900a) is then at least partially in disagreement with that provided subsequently (Bellini 1915), led to the conclusion that it is here considered a nomen dubium (ICZN 2012: glossary). ...
... Monacha cantiana, as well as H. cerioi (see above for a taxonomic status similar to that of H. bellinii), were also considered originally uncommon in Capri Island by Bellini (1900a); Petraccioli et al. (2005Petraccioli et al. ( , 2006 did not find it during recent samplings held in Capri Island, and only listed Monacha cartusiana (Müller, 1774) as present. Noteworthy, species-level taxonomy of the genus Monacha Fitzinger, 1833, and particularly of the M. cantiana species complex, is still unresolved (Welter-Schultes 2012;Neiber & Hausdorf 2016;Pieńkowska et al. 2018Pieńkowska et al. , 2019. Apart of that, the absence of type material and even figures in any paper published by Bellini, as well as the taxonomic status of H. cerioi to which it is compared (see above), led to the conclusion that it is considered a nomen dubium (ICZN 2012: glossary). ...
Raffaello Bellini (1874-1930) was an almost unknown Italian naturalist who produced a wide scientific literature. He followed the "Golden Age" of the Neapolitan Malacological School and his malacological papers were particularly related to Italy, focusing on marine, freshwater, fossil, and terrestrial species. We hereby highlight and summarize Raffaello Bellini's biography and malacological work, and revise the status of recent molluscan taxa described by him. Our bibliographic research revealed that from 1897 to 1929 Bellini published 41 papers dealing with malacology and altogether instituted 46 molluscan nominal taxa, 17 of which are based on fossil samples and 29 on recent samples. Within the latter group, 24 (13 marine, nine terrestrial, and two freshwater) taxa are potentially available, whilst five (two marine and three terrestrial) are nomina nuda. Among these 24 taxa, 13 are available, ten have infrasubspecific rank and thus are not available, and one is permanently invalid. Among available binomial names, seven are junior synonyms of valid taxa and six are nomina dubia. Gibbula umbilicaris var. acherusiae Bellini, 1903 is here considered a junior synonym of Gibbula ardens (Salis Marschlins, 1793) for the first time. Finally, three recent marine molluscan taxa were named after Raffaello Bellini. However, none of these species proved to be valid. The present paper sheds light on the work done by the latest of the "early" authors of the Neapolitan Malacological School and contributes to nomenclature and taxonomy of Recent European molluscan taxa.
... En effet, Pieńkowska et al. (2018aPieńkowska et al. ( , 2019 (Risso, 1826) du sud-est de la France. Cela confirme l'hypothèse formulée par Au final, ces résultats semblent confirmer l'origine sud européenne de M. cantiana sensu stricto et l'hypothèse de sa dispersion par les activités humaines vers le nord-ouest européen, en Angleterre particulièrement, et ce, dès la fin de la période romaine. ...
... Nous fournissons ici la description de l'espèce en suivant les caractères fournis par Kerney et al. (1999), Welter-Schultes (2012) et Pieńkowska et al. (2018aPieńkowska et al. ( , 2019. Les coquilles des individus adultes présentent une forme sub-globuleuse à globuleuse ( Figure 1A). ...
Résumé ̶ Originaire du sud de l'Europe, Monacha cantiana (Montagu, 1803) présente actuellement une aire de répartition sud et ouest-européenne jusqu'en Angleterre où elle semble coloniser des zones géographiques de plus en plus septentrionales. Cependant, cette espèce nécessite pour sa détermination une approche conjointe morpho-anatomique qui permet entre autres de la distinguer de Monacha cemenelea (Risso, 1826) dont le statut d'espèce valide a été confirmé récemment. Nous fournissons ici une synthèse sur le développement des connaissances concernant cette espèce, le détail de ses descripteurs spécifiques et les informations afférentes à la découverte de deux nouvelles populations dans la région Grand Est.
Mots-clés ̶ espèce cryptique, introduction, genre Monacha, délimitation d'espèce, Monacha cantiana, Monacha cemenelea.
Abstract ̶ Note on the taxonomy of Monacha cantiana (Montagu, 1803) (Mollusca, Gastropoda, Hygromiidae) and new occurrence data near Longwy (eastern France)
Originating from southern Europe, Monacha cantiana (Montagu, 1803) has currently a southern and western European distribution range as far as England, where it seems to colonize increasingly northern geographical areas. However, this species requires a morpho-anatomical approach to distinguish it from Monacha cemenelea (Risso, 1826), which has recently been confirmed to be a valid species. We provide here a synthesis on the species delimitation of this taxon with the specific descriptors and information related to the discovery of two new populations from north-eastern France.
Keywords ̶ cryptic species, introduction, Monacha genus, species delimitation, Monacha cantiana, Monacha cemenelea.
