Figure2: Sporulation on lettuce leaves and sporangia-sporangiophore of Bremia lactucae 

Contexts in source publication

Context 1

... suspensions prepared in MS media were placed into sterile glass petri dishes after which the leaves were placed so that the lower surfaces were in contact with the spore suspension. The petri dishes were covered with a stretch film (Fig. 3a). A series of petri dishes were incubated in a growth room at 13±1 o C, with a 12 h photoperiod and an another series were incubated at18-21 o C with a 12 h photoperiod. ...

Context 2

... this experiment, spore suspensions prepared in MS medium of Bremia lactucae, which obtained better results in previous studies, were used. On the sterilized slides, rings of 1 cm in diameter were made with wax and a spore suspension of about 50 μm was placed in these rings. Then leaf discs of 7 mm in diameter, which were cut with cork borer from the leaves of 15-day (2-3 leaf) plants, were placed so that their lower surfaces were in contact with this suspension. The slides thus prepared were placed in petri dishes containing sterile moist filter paper and incubated (Fig. 3a). A series of petri dishes were placed in an incubation chamber containing 12 hours of light 12 hours of dark period and 13 ± 1 ° C of temperature, a series of 12 hours of light 12 hours of dark period and 18-21 °C of ...

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... this method, moist filter papers were placed in 20 cm diameter sterile glass petri dishes, and the youngest leaves of the 2-3-leaf susceptible lettuce varieties were placed in petri dishes so that their upper surfaces contact the filter papers (Fig. 3b). In the blotter method, as an alternatively, instead of the whole plant leaf, 1 cm diameter the youngest lettuce leaves disks were placed on the filter papers. For the inoculation of the leaves, spore suspensions prepared in distilled water, MS medium, and tap water were used. All of these have been conducted as separate trials. According to the size of the leaf area to be treated using sterile Pasteur pipette, the prepared spores suspensions were placed in contact with the lower surface of 1 and / or 2 drops of leaves and the petri dishes were covered with stretch film. Care was taken to keep the drops (not to fall off from the surface of the leaf) on the leaves. The petri dishes were incubated for 2 weeks in the growth room at 13±1 o C, with a 12 h photoperiod. Inoculation using a spore suspension prepared in MS medium was repeated using a parallel and placed into an incubation chamber that contains 12 light /12 h dark periods and 18-21 o C ...

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... this method, leaf discs (2 cm in diameter) were placed on 2% water agar so that their upper surfaces are in contact with the agar. Spore suspensions prepared in MS medium were placed on the lower surface of leaf discs with the help of a Pasteur pipette. The petri dish containing two leaf discs was wrapped with a stretch film (Fig. 3d). The petri dishes were incubated at the two different temperature conditions as mentioned ...