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Figure no. 1: Plant of Lindenbergia indica (Linn.) Kuntz
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Antioxidants are naturally occurring plant substances that protect the body from damage caused by harmful molecules called free radicals and prevent oxidation, which can damage cells and may contribute to aging. Lindenbergia indica (Linn.) Kuntze is a perennial herb belongs to Scrophulariaceae family from the Himalayas, found to grow on damp, old w...
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... extract was redissolved in 70% ethanol. The 5 ml assay mixture contained 3.98 ml methanol, 20µl extract (50µl, 100µl, 150µl, 200µl), and 1 ml DPPH (0.15 mM in methanol). The mixture was incubated for 30 minutes in the dark at room temperature and the decrease in absorbance was measured at 517 nm using a spectrophotometer. ...Context 2
... Free radical scavenging effect of plant extract was determined using the stable scavenger 2,2-diphenyl-1- picrylhydrazyl (DPPH). The extract was redissolved in 70% ethanol. The 5 ml assay mixture contained 3.98 ml methanol, 20µl extract (50µl, 100µl, 150µl, 200µl), and 1 ml DPPH (0.15 mM in methanol). The mixture was incubated for 30 minutes in the dark at room temperature and the decrease in absorbance was measured at 517 nm using a spectrophotometer. Ascorbic acid was uses as references. The degree of DPPH purple decolorization to DPPH yellow indicated the scavenging efficiency of the extract. Lower absorbance of the reaction mixture indicated higher free radical scavenging activity. The scavenging activity against DPPH was calculated using the equation. DPPH scavenging activity (%) = (A0-A1) / A0× 100 A0 is the absorbance of the control reaction (ethanol with DPPH solution), A1 is the absorbance of the test sample. The result was analyzed in triplicate. The IC50 value is the concentration of the sample required to inhibit 50% of the DPPH free ...Similar publications
The present investigation was carried out on phytochemical characterization and antibacterial activity of Lindenbergia indica Vatke, belongs to family Scrophulariaceae, is a perennial herb, found to grow on bare rocks and old brick walls in Doon valley. Ethnobotanical studies shows that juice of this plant is used traditionally in chronic bronchiti...
Citations
... Lifestyle factors like exposure to toxic chemicals, air pollution, smoking, consumption of alcohol and unhealthy fried or junk foods not only triggers the free radical production but are also linked to diseases such as cancer and cardiovascular diseases. Free radicals are derived either from normal essential metabolic processes in the human body or from external sources such as exposure to X-rays, ozone, cigarette smoking, air pollutants, and industrial chemicals (Bagchi and Puri,1998;Kumpulainen and Salonen,1999;Pourmorad et al., 2006;Singh and Aswal, 2018) and free radicals can cause oxidative stress, a process that can trigger cell damage. Antioxidant agents can act against free radicals either by retarding their formation (preventive antioxidants) or by in-activating in reaction medium (chain breaking antioxidants) (Singh and Aswal, 2018). ...
... Free radicals are derived either from normal essential metabolic processes in the human body or from external sources such as exposure to X-rays, ozone, cigarette smoking, air pollutants, and industrial chemicals (Bagchi and Puri,1998;Kumpulainen and Salonen,1999;Pourmorad et al., 2006;Singh and Aswal, 2018) and free radicals can cause oxidative stress, a process that can trigger cell damage. Antioxidant agents can act against free radicals either by retarding their formation (preventive antioxidants) or by in-activating in reaction medium (chain breaking antioxidants) (Singh and Aswal, 2018). Antioxidants are broadly divided into by two groups on the basis of their occurrence i.e., naturally or synthetic. ...
In present day scenario, free radicals are produced naturally in human body linked to diseases such as cancer and cardiovascular diseases. Antioxidant agents can act against free radicals either by retarding their formation (preventive antioxidants) or by in-activating in reaction medium (chain breaking antioxidants). Thus, the search for effective, nontoxic natural compounds with antioxidative activity has been intensified in recent years. Stinging nettle, (Urtica dioica L), also called common nettle, weedy perennial plant of the nettle family (Urticaceae), and known for its stinging leaves. The present study comprised of collection of plant Urtica dioica L from different locations of Uttarakhand followed by ethanol extraction for phytochemical analysis. Estimation of total phenol and flavonoid contents with the emphasis for determination of antioxidant activities of these plants. The percentage yield of crude extract in Urtica sample ranges from 58-64%. Phytochemical studies showed that alkaloids, flavonoids, carbohydrates, proteins, tannins, saponins, terpenoids, steroids, glycosides were present in all the samples of Urtica dioica while anthocyanin and cardiac glycosides were absent in all the samples. The phenolic concentration of samples ranges from 0.44±0.03 to 0.64±0.02. The flavonoid content of the samples of Urtica dioica L varies from 0.83±0.23 to 0.68±0.009. The study was also conducted with reference to the reducing power activity which was highest in sample 3 which is of Pauri. The antioxidant activities of three samples were calculated by DPPH scavenging activity. The IC50 value of samples ranged from 227.191-389.066 µg/ml. Thus, the plant Urtica dioica L. can be used as a food supplement and herbal antioxidants which can reduce the effect of free radical and maintaining the health.
