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4) MTHFR gene locus 2.5.2.2. C677T polymorphism C677T is a missense mutation in exon 4 of the MTHFR gene,
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This study was focused on the genetic and molecular abnormalities that might contribute to recurrent spontaneous abortion (RSA) and delineates strategies for genetic evaluation and clinical management in subsequent pregnancies. It was also aimed to investigate chromosomal aberrations, micronucleus formation, sister chromatid exchanges and methylent...
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Introduction
Miscarriage increases women's risk for psychiatric disorders such as depression, anxiety and stress and can also lead to a threat to life. Hospital environment and provision of appropriate clinical information to the client is an important determinant in prevention of psychological distress. The aim of our study was to describe the ps...
Citations
... When photographing the electrophoresis, these pieces appear guilty. (Comet) With heads distinct contained on the DNA and proper tail containing pieces of DNA damaged (17). ...
Background: The comet analysis (electromagnetic relay analysis of a single cell) is ahighly sensitive method for determining DNA damage because to exposure tocarcinogens and other substances that consequently affect fertility.Objective: the aim of this study is to Evaluation the broken DNA in male diabeticmice. Created by alloxan and knowledge of the effect of some nutritional systems onthe treatment and repair of genetic material due to diabetes.Materials and methods: In this study were used, 42 male Albino mice labs at the ageof 2-3 months and weight (25-30) g then 6 animals were isolated to represent thecontrol group, The remaining animals were injected between the thighs by alloxan 150mg / kg and left the next day to make sure hit by diabetes and randomly distributed tosix groups in addition to a set of Not affected control transactions.Results and conclusion: It was found from the results of the statistical analysis shownin figure(1) showed the presence of the highest percentage of injuries in the geneticmaterial that was in the group of animals with diabetes and untreated for the durationof the experiment, as it was clear from the photos of this group that the tail lengthresulting from the migration of the genetic material DNA to outside the nucleusbecause to its damage, as well as the results showed that the group of animals affectedby diabetes and the treatment by adding the Salvia officinalis plant to both plant andanimal nutrition had a significant decrease (<0.05> P) in the proportion of geneticmaterial injuries where the proportion of high (long guilt) infections was low in thesetwo The two groups compared to the group of animals with diabetes and untreated forthe duration of the experiment.
... These parameters have been widely used by many workers for genotoxic studies (27). Comet assay is useful technique to determine DNA damage which may be cause RSA (21,22), DNA Fragmentation in Male Couples of females with RSA (23,10). ...
... The results of comet assay indicate that tail length, DNA and moment were significantly (p≤0.05) high in RSA women in comparison with its counterpart control group (22). ...
The cytokinesis-block micronucleus assay is one of the most widely used methods to determine DNA damage caused by exposure to mutagenic, carcinogenic and environmental agents that affect women infertility. This study was aimed to assess possible genomic instability in women with recurrent spontaneous abortion (RSA). Fifty blood samples were collected from women complaining with RSA and 25 normal fertile females, who had at least one or more child. Cytogenetic study shows micronuclei frequency of peripheral blood lymphocytes is significantly decreased in comparison with that of normal pregnant women, while the nuclear division index shows no significant signs in all blood samples of aborted women.
